Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06880 and MMP_RS06885 are separated by 382 nucleotides MMP_RS06885 and MMP_RS06890 are separated by 19 nucleotides MMP_RS06890 and MMP_RS06895 are separated by 10 nucleotides
MMP_RS06880: MMP_RS06880 - chorismate synthase, at 1,313,078 to 1,314,199
_RS06880
MMP_RS06885: MMP_RS06885 - 50S ribosome-binding GTPase, at 1,314,582 to 1,315,736
_RS06885
MMP_RS06890: MMP_RS06890 - mevalonate kinase, at 1,315,756 to 1,316,709
_RS06890
MMP_RS06895: MMP_RS06895 - selenocysteine-specific translation elongation factor, at 1,316,720 to 1,318,126
_RS06895
Position (kb)
1314
1315
1316 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1314.178 kb on - strand at 1314.197 kb on - strand at 1314.198 kb on + strand at 1314.286 kb on - strand at 1314.287 kb on + strand at 1314.313 kb on + strand at 1314.321 kb on - strand at 1314.321 kb on - strand at 1314.321 kb on - strand at 1314.373 kb on + strand at 1314.448 kb on + strand at 1314.504 kb on + strand at 1314.504 kb on + strand at 1314.553 kb on + strand at 1314.559 kb on - strand at 1314.560 kb on + strand at 1314.564 kb on - strand at 1314.566 kb on - strand at 1314.566 kb on - strand at 1314.567 kb on + strand at 1314.567 kb on + strand at 1314.569 kb on + strand at 1314.569 kb on + strand at 1314.569 kb on + strand at 1314.569 kb on + strand at 1314.590 kb on - strand at 1314.741 kb on - strand, within MMP_RS06885 at 1314.771 kb on - strand, within MMP_RS06885 at 1314.926 kb on + strand, within MMP_RS06885 at 1315.212 kb on + strand, within MMP_RS06885 at 1315.280 kb on + strand, within MMP_RS06885 at 1316.083 kb on - strand at 1316.104 kb on + strand at 1316.147 kb on + strand at 1316.274 kb on + strand at 1316.539 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 1,314,178 - -3.5 1,314,197 - +0.1 1,314,198 + +0.1 1,314,286 - +0.5 1,314,287 + +0.4 1,314,313 + +0.5 1,314,321 - +0.7 1,314,321 - +0.2 1,314,321 - -1.1 1,314,373 + +0.5 1,314,448 + +1.1 1,314,504 + +0.7 1,314,504 + +0.2 1,314,553 + -0.1 1,314,559 - -0.0 1,314,560 + -0.1 1,314,564 - +0.3 1,314,566 - +0.4 1,314,566 - +0.3 1,314,567 + +0.4 1,314,567 + +0.1 1,314,569 + +0.6 1,314,569 + -0.7 1,314,569 + +0.6 1,314,569 + -0.1 1,314,590 - -0.4 1,314,741 - MMP_RS06885 0.14 -2.0 1,314,771 - MMP_RS06885 0.16 -1.3 1,314,926 + MMP_RS06885 0.30 -0.3 1,315,212 + MMP_RS06885 0.55 -0.6 1,315,280 + MMP_RS06885 0.60 -1.2 1,316,083 - +0.9 1,316,104 + -2.8 1,316,147 + -3.1 1,316,274 + -0.6 1,316,539 - -0.9
Or see this region's nucleotide sequence