Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06835 and MMP_RS06840 are separated by 38 nucleotides MMP_RS06840 and MMP_RS06845 are separated by 37 nucleotides MMP_RS06845 and MMP_RS06850 are separated by 37 nucleotides MMP_RS06850 and MMP_RS06855 are separated by 31 nucleotides MMP_RS06855 and MMP_RS06860 are separated by 47 nucleotides MMP_RS06860 and MMP_RS06865 are separated by 25 nucleotides MMP_RS06865 and MMP_RS06870 are separated by 96 nucleotides
MMP_RS06835: MMP_RS06835 - 30S ribosomal protein S9, at 1,308,765 to 1,309,169
_RS06835
MMP_RS06840: MMP_RS06840 - DNA-directed RNA polymerase subunit N, at 1,309,208 to 1,309,414
_RS06840
MMP_RS06845: MMP_RS06845 - tRNA-Pro, at 1,309,452 to 1,309,525
_RS06845
MMP_RS06850: MMP_RS06850 - DNA-directed RNA polymerase subunit K, at 1,309,563 to 1,309,736
_RS06850
MMP_RS06855: MMP_RS06855 - hypothetical protein, at 1,309,768 to 1,310,703
_RS06855
MMP_RS06860: MMP_RS06860 - 4Fe-4S binding protein, at 1,310,751 to 1,310,939
_RS06860
MMP_RS06865: MMP_RS06865 - HypC/HybG/HupF family hydrogenase formation chaperone, at 1,310,965 to 1,311,231
_RS06865
MMP_RS06870: MMP_RS06870 - DedA family protein, at 1,311,328 to 1,311,801
_RS06870
Position (kb) 1309
1310
1311 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1309.168 kb on + strand at 1309.387 kb on + strand, within MMP_RS06840 at 1309.407 kb on + strand at 1309.425 kb on + strand at 1309.432 kb on - strand at 1309.436 kb on + strand at 1309.540 kb on + strand at 1309.672 kb on - strand at 1309.742 kb on - strand at 1309.836 kb on + strand at 1309.846 kb on + strand at 1309.870 kb on - strand, within MMP_RS06855 at 1309.925 kb on - strand, within MMP_RS06855 at 1309.957 kb on - strand, within MMP_RS06855 at 1309.985 kb on - strand, within MMP_RS06855 at 1310.195 kb on - strand, within MMP_RS06855 at 1310.199 kb on - strand, within MMP_RS06855 at 1310.199 kb on - strand, within MMP_RS06855 at 1310.204 kb on + strand, within MMP_RS06855 at 1310.266 kb on + strand, within MMP_RS06855 at 1310.319 kb on - strand, within MMP_RS06855 at 1310.319 kb on - strand, within MMP_RS06855 at 1310.374 kb on + strand, within MMP_RS06855 at 1310.402 kb on - strand, within MMP_RS06855 at 1310.519 kb on + strand, within MMP_RS06855 at 1310.521 kb on - strand, within MMP_RS06855 at 1310.591 kb on - strand, within MMP_RS06855 at 1310.592 kb on + strand, within MMP_RS06855 at 1310.632 kb on + strand at 1310.691 kb on - strand at 1310.721 kb on - strand at 1310.722 kb on + strand at 1310.723 kb on - strand at 1310.723 kb on - strand at 1310.724 kb on + strand at 1310.730 kb on - strand at 1310.851 kb on - strand, within MMP_RS06860 at 1310.881 kb on - strand, within MMP_RS06860 at 1310.937 kb on - strand at 1310.941 kb on - strand at 1311.007 kb on - strand, within MMP_RS06865 at 1311.098 kb on - strand, within MMP_RS06865 at 1311.099 kb on + strand, within MMP_RS06865 at 1311.209 kb on + strand at 1311.258 kb on - strand at 1311.267 kb on - strand at 1311.273 kb on - strand at 1311.295 kb on + strand at 1311.343 kb on + strand at 1311.435 kb on - strand, within MMP_RS06870 at 1311.480 kb on - strand, within MMP_RS06870 at 1311.517 kb on + strand, within MMP_RS06870 at 1311.543 kb on + strand, within MMP_RS06870 at 1311.556 kb on + strand, within MMP_RS06870 at 1311.556 kb on + strand, within MMP_RS06870 at 1311.602 kb on - strand, within MMP_RS06870 at 1311.608 kb on + strand, within MMP_RS06870
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 1,309,168 + -2.3 1,309,387 + MMP_RS06840 0.86 -0.3 1,309,407 + -0.4 1,309,425 + -0.9 1,309,432 - -0.7 1,309,436 + -0.4 1,309,540 + -2.6 1,309,672 - -1.6 1,309,742 - -0.1 1,309,836 + -0.1 1,309,846 + -0.2 1,309,870 - MMP_RS06855 0.11 -0.2 1,309,925 - MMP_RS06855 0.17 +0.1 1,309,957 - MMP_RS06855 0.20 -0.4 1,309,985 - MMP_RS06855 0.23 -0.1 1,310,195 - MMP_RS06855 0.46 -0.8 1,310,199 - MMP_RS06855 0.46 -1.1 1,310,199 - MMP_RS06855 0.46 -0.4 1,310,204 + MMP_RS06855 0.47 -0.8 1,310,266 + MMP_RS06855 0.53 -1.0 1,310,319 - MMP_RS06855 0.59 -1.0 1,310,319 - MMP_RS06855 0.59 +0.1 1,310,374 + MMP_RS06855 0.65 -0.9 1,310,402 - MMP_RS06855 0.68 -0.6 1,310,519 + MMP_RS06855 0.80 +0.1 1,310,521 - MMP_RS06855 0.80 -0.9 1,310,591 - MMP_RS06855 0.88 +0.1 1,310,592 + MMP_RS06855 0.88 -0.0 1,310,632 + -0.4 1,310,691 - -0.4 1,310,721 - +0.5 1,310,722 + +0.3 1,310,723 - +0.5 1,310,723 - +0.0 1,310,724 + +0.3 1,310,730 - +0.0 1,310,851 - MMP_RS06860 0.53 +0.0 1,310,881 - MMP_RS06860 0.69 -0.4 1,310,937 - +0.5 1,310,941 - +0.3 1,311,007 - MMP_RS06865 0.16 -0.1 1,311,098 - MMP_RS06865 0.50 -1.8 1,311,099 + MMP_RS06865 0.50 -1.8 1,311,209 + -2.0 1,311,258 - -0.7 1,311,267 - -0.2 1,311,273 - -0.7 1,311,295 + -0.0 1,311,343 + -0.3 1,311,435 - MMP_RS06870 0.23 -0.2 1,311,480 - MMP_RS06870 0.32 -0.2 1,311,517 + MMP_RS06870 0.40 -0.1 1,311,543 + MMP_RS06870 0.45 -1.0 1,311,556 + MMP_RS06870 0.48 +2.9 1,311,556 + MMP_RS06870 0.48 -0.3 1,311,602 - MMP_RS06870 0.58 -0.4 1,311,608 + MMP_RS06870 0.59 -0.2
Or see this region's nucleotide sequence