Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06360 and MMP_RS06365 are separated by 30 nucleotides MMP_RS06365 and MMP_RS06370 are separated by 78 nucleotides MMP_RS06370 and MMP_RS06375 overlap by 4 nucleotides MMP_RS06375 and MMP_RS06380 are separated by 27 nucleotides
MMP_RS06360: MMP_RS06360 - UPF0280 family protein, at 1,222,830 to 1,223,573
_RS06360
MMP_RS06365: MMP_RS06365 - hypothetical protein, at 1,223,604 to 1,223,804
_RS06365
MMP_RS06370: MMP_RS06370 - biotin synthase BioB, at 1,223,883 to 1,224,866
_RS06370
MMP_RS06375: MMP_RS06375 - ATP-dependent sacrificial sulfur transferase LarE, at 1,224,863 to 1,225,639
_RS06375
MMP_RS06380: MMP_RS06380 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,225,667 to 1,226,809
_RS06380
Position (kb)
1223
1224
1225 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1222.926 kb on + strand at 1222.982 kb on - strand at 1223.426 kb on - strand at 1223.427 kb on + strand at 1223.577 kb on + strand at 1223.577 kb on + strand at 1223.577 kb on + strand at 1223.582 kb on - strand at 1223.583 kb on + strand at 1223.583 kb on + strand at 1223.584 kb on - strand at 1223.584 kb on - strand at 1223.585 kb on + strand at 1223.585 kb on + strand at 1223.587 kb on - strand at 1223.588 kb on + strand at 1223.588 kb on + strand at 1223.588 kb on + strand at 1223.588 kb on + strand at 1223.589 kb on - strand at 1223.589 kb on - strand at 1223.589 kb on - strand at 1223.589 kb on - strand at 1223.589 kb on - strand at 1223.590 kb on + strand at 1223.590 kb on + strand at 1223.590 kb on + strand at 1223.598 kb on - strand at 1223.599 kb on + strand at 1223.622 kb on + strand at 1223.627 kb on - strand, within MMP_RS06365 at 1223.667 kb on + strand, within MMP_RS06365 at 1223.834 kb on + strand at 1224.010 kb on + strand, within MMP_RS06370 at 1224.221 kb on + strand, within MMP_RS06370 at 1224.364 kb on - strand, within MMP_RS06370 at 1224.417 kb on + strand, within MMP_RS06370 at 1224.448 kb on - strand, within MMP_RS06370 at 1224.577 kb on - strand, within MMP_RS06370 at 1224.897 kb on + strand at 1224.933 kb on - strand at 1224.971 kb on + strand, within MMP_RS06375 at 1224.971 kb on + strand, within MMP_RS06375 at 1225.246 kb on + strand, within MMP_RS06375 at 1225.378 kb on - strand, within MMP_RS06375 at 1225.485 kb on + strand, within MMP_RS06375 at 1225.570 kb on + strand at 1225.582 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 1,222,926 + -1.1 1,222,982 - -1.5 1,223,426 - -2.3 1,223,427 + -1.5 1,223,577 + -0.3 1,223,577 + +0.0 1,223,577 + -0.1 1,223,582 - +0.0 1,223,583 + +0.0 1,223,583 + +1.1 1,223,584 - +0.4 1,223,584 - +0.0 1,223,585 + +0.0 1,223,585 + -0.3 1,223,587 - +0.6 1,223,588 + +0.2 1,223,588 + -0.0 1,223,588 + +1.4 1,223,588 + -0.4 1,223,589 - +0.4 1,223,589 - +0.4 1,223,589 - +0.3 1,223,589 - +0.2 1,223,589 - +0.6 1,223,590 + -0.2 1,223,590 + -0.4 1,223,590 + -0.2 1,223,598 - +0.3 1,223,599 + +0.0 1,223,622 + +0.0 1,223,627 - MMP_RS06365 0.11 +0.1 1,223,667 + MMP_RS06365 0.31 -0.3 1,223,834 + +0.2 1,224,010 + MMP_RS06370 0.13 -0.4 1,224,221 + MMP_RS06370 0.34 +2.1 1,224,364 - MMP_RS06370 0.49 -0.7 1,224,417 + MMP_RS06370 0.54 -0.3 1,224,448 - MMP_RS06370 0.57 -0.8 1,224,577 - MMP_RS06370 0.71 -0.7 1,224,897 + +0.7 1,224,933 - +0.8 1,224,971 + MMP_RS06375 0.14 +0.1 1,224,971 + MMP_RS06375 0.14 +0.1 1,225,246 + MMP_RS06375 0.49 +0.2 1,225,378 - MMP_RS06375 0.66 +0.5 1,225,485 + MMP_RS06375 0.80 +0.1 1,225,570 + +1.4 1,225,582 - -1.0
Or see this region's nucleotide sequence