Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04525 and MMP_RS04530 are separated by 41 nucleotides MMP_RS04530 and MMP_RS04535 are separated by 120 nucleotides
MMP_RS04525: MMP_RS04525 - D-2-hydroxyacid dehydrogenase, at 861,628 to 862,584
_RS04525
MMP_RS04530: MMP_RS04530 - ABC transporter permease, at 862,626 to 863,819
_RS04530
MMP_RS04535: MMP_RS04535 - hydroxymethylbilane synthase, at 863,940 to 864,854
_RS04535
Position (kb)
862
863
864 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 861.642 kb on + strand at 861.715 kb on - strand at 861.715 kb on - strand at 861.716 kb on + strand at 861.740 kb on - strand, within MMP_RS04525 at 861.774 kb on - strand, within MMP_RS04525 at 861.775 kb on + strand, within MMP_RS04525 at 861.780 kb on - strand, within MMP_RS04525 at 861.781 kb on + strand, within MMP_RS04525 at 861.786 kb on + strand, within MMP_RS04525 at 861.786 kb on + strand, within MMP_RS04525 at 861.825 kb on + strand, within MMP_RS04525 at 861.829 kb on - strand, within MMP_RS04525 at 861.829 kb on - strand, within MMP_RS04525 at 861.829 kb on - strand, within MMP_RS04525 at 861.830 kb on + strand, within MMP_RS04525 at 861.840 kb on - strand, within MMP_RS04525 at 861.853 kb on - strand, within MMP_RS04525 at 861.894 kb on + strand, within MMP_RS04525 at 861.900 kb on + strand, within MMP_RS04525 at 861.945 kb on + strand, within MMP_RS04525 at 861.956 kb on - strand, within MMP_RS04525 at 861.956 kb on - strand, within MMP_RS04525 at 861.957 kb on + strand, within MMP_RS04525 at 861.985 kb on - strand, within MMP_RS04525 at 861.985 kb on - strand, within MMP_RS04525 at 861.986 kb on + strand, within MMP_RS04525 at 861.986 kb on + strand, within MMP_RS04525 at 861.987 kb on - strand, within MMP_RS04525 at 861.999 kb on + strand, within MMP_RS04525 at 862.145 kb on - strand, within MMP_RS04525 at 862.145 kb on - strand, within MMP_RS04525 at 862.145 kb on - strand, within MMP_RS04525 at 862.146 kb on + strand, within MMP_RS04525 at 862.146 kb on + strand, within MMP_RS04525 at 862.146 kb on + strand, within MMP_RS04525 at 862.152 kb on + strand, within MMP_RS04525 at 862.152 kb on + strand, within MMP_RS04525 at 862.446 kb on + strand, within MMP_RS04525 at 862.446 kb on + strand, within MMP_RS04525 at 862.456 kb on + strand, within MMP_RS04525 at 862.461 kb on - strand, within MMP_RS04525 at 862.461 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.466 kb on + strand, within MMP_RS04525 at 862.466 kb on + strand, within MMP_RS04525 at 862.466 kb on + strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.468 kb on + strand, within MMP_RS04525 at 862.468 kb on + strand, within MMP_RS04525 at 862.468 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.564 kb on + strand at 862.566 kb on + strand at 862.578 kb on - strand at 862.585 kb on - strand at 862.589 kb on - strand at 862.594 kb on + strand at 862.594 kb on + strand at 862.667 kb on + strand at 862.693 kb on - strand at 862.714 kb on - strand at 862.714 kb on - strand at 862.715 kb on + strand at 862.715 kb on + strand at 862.718 kb on - strand at 862.767 kb on - strand, within MMP_RS04530 at 862.771 kb on - strand, within MMP_RS04530 at 862.771 kb on - strand, within MMP_RS04530 at 862.771 kb on - strand, within MMP_RS04530 at 862.883 kb on + strand, within MMP_RS04530 at 862.976 kb on + strand, within MMP_RS04530 at 863.006 kb on + strand, within MMP_RS04530 at 863.263 kb on + strand, within MMP_RS04530 at 863.263 kb on + strand, within MMP_RS04530 at 863.267 kb on - strand, within MMP_RS04530 at 863.309 kb on + strand, within MMP_RS04530 at 863.510 kb on - strand, within MMP_RS04530 at 863.728 kb on - strand at 863.740 kb on - strand at 863.740 kb on - strand at 863.740 kb on - strand at 863.752 kb on - strand at 863.753 kb on + strand at 863.753 kb on + strand at 863.760 kb on - strand at 863.801 kb on - strand at 863.802 kb on + strand at 863.836 kb on + strand at 863.847 kb on + strand at 863.849 kb on - strand at 863.874 kb on + strand at 863.915 kb on + strand at 863.920 kb on + strand at 863.924 kb on + strand at 863.969 kb on - strand at 863.970 kb on + strand at 863.994 kb on - strand at 863.995 kb on + strand at 864.010 kb on - strand at 864.011 kb on + strand at 864.047 kb on - strand at 864.048 kb on + strand at 864.075 kb on + strand at 864.245 kb on + strand at 864.290 kb on + strand at 864.424 kb on - strand at 864.425 kb on + strand at 864.700 kb on - strand at 864.729 kb on + strand at 864.763 kb on - strand at 864.766 kb on - strand at 864.767 kb on + strand at 864.771 kb on + strand at 864.771 kb on + strand at 864.771 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 861,642 + -0.2 861,715 - +0.3 861,715 - +0.0 861,716 + -0.5 861,740 - MMP_RS04525 0.12 -0.2 861,774 - MMP_RS04525 0.15 +0.1 861,775 + MMP_RS04525 0.15 +0.1 861,780 - MMP_RS04525 0.16 +0.7 861,781 + MMP_RS04525 0.16 +0.4 861,786 + MMP_RS04525 0.17 +0.0 861,786 + MMP_RS04525 0.17 +0.3 861,825 + MMP_RS04525 0.21 -0.1 861,829 - MMP_RS04525 0.21 +0.2 861,829 - MMP_RS04525 0.21 -0.2 861,829 - MMP_RS04525 0.21 -0.4 861,830 + MMP_RS04525 0.21 -0.0 861,840 - MMP_RS04525 0.22 +0.4 861,853 - MMP_RS04525 0.24 +0.5 861,894 + MMP_RS04525 0.28 -0.1 861,900 + MMP_RS04525 0.28 +0.1 861,945 + MMP_RS04525 0.33 +0.1 861,956 - MMP_RS04525 0.34 +0.2 861,956 - MMP_RS04525 0.34 -0.7 861,957 + MMP_RS04525 0.34 +0.6 861,985 - MMP_RS04525 0.37 -0.0 861,985 - MMP_RS04525 0.37 +0.2 861,986 + MMP_RS04525 0.37 +0.4 861,986 + MMP_RS04525 0.37 +0.0 861,987 - MMP_RS04525 0.38 +0.5 861,999 + MMP_RS04525 0.39 -0.4 862,145 - MMP_RS04525 0.54 -0.2 862,145 - MMP_RS04525 0.54 +0.1 862,145 - MMP_RS04525 0.54 -0.0 862,146 + MMP_RS04525 0.54 -0.3 862,146 + MMP_RS04525 0.54 +0.2 862,146 + MMP_RS04525 0.54 +0.1 862,152 + MMP_RS04525 0.55 -0.1 862,152 + MMP_RS04525 0.55 -0.0 862,446 + MMP_RS04525 0.85 -0.1 862,446 + MMP_RS04525 0.85 +0.2 862,456 + MMP_RS04525 0.87 +0.1 862,461 - MMP_RS04525 0.87 +0.4 862,461 - MMP_RS04525 0.87 -0.4 862,465 - MMP_RS04525 0.87 -0.2 862,465 - MMP_RS04525 0.87 -0.0 862,465 - MMP_RS04525 0.87 +0.2 862,465 - MMP_RS04525 0.87 +0.5 862,466 + MMP_RS04525 0.88 +0.1 862,466 + MMP_RS04525 0.88 -0.2 862,466 + MMP_RS04525 0.88 +0.2 862,467 - MMP_RS04525 0.88 +0.9 862,467 - MMP_RS04525 0.88 -0.1 862,467 - MMP_RS04525 0.88 +0.2 862,467 - MMP_RS04525 0.88 +0.4 862,468 + MMP_RS04525 0.88 -0.2 862,468 + MMP_RS04525 0.88 -0.1 862,468 + MMP_RS04525 0.88 -0.2 862,470 + MMP_RS04525 0.88 -0.3 862,470 + MMP_RS04525 0.88 +0.7 862,470 + MMP_RS04525 0.88 -0.2 862,470 + MMP_RS04525 0.88 -0.2 862,564 + +0.1 862,566 + +0.1 862,578 - -0.1 862,585 - +0.3 862,589 - -0.1 862,594 + +0.2 862,594 + +0.0 862,667 + +0.5 862,693 - +0.1 862,714 - +0.3 862,714 - +0.4 862,715 + +0.0 862,715 + +0.4 862,718 - -0.1 862,767 - MMP_RS04530 0.12 +0.5 862,771 - MMP_RS04530 0.12 -0.2 862,771 - MMP_RS04530 0.12 +0.0 862,771 - MMP_RS04530 0.12 +0.1 862,883 + MMP_RS04530 0.22 -0.1 862,976 + MMP_RS04530 0.29 +0.1 863,006 + MMP_RS04530 0.32 -0.4 863,263 + MMP_RS04530 0.53 -0.1 863,263 + MMP_RS04530 0.53 -0.1 863,267 - MMP_RS04530 0.54 +0.0 863,309 + MMP_RS04530 0.57 +0.4 863,510 - MMP_RS04530 0.74 +0.2 863,728 - +0.0 863,740 - +0.0 863,740 - -0.4 863,740 - -0.1 863,752 - -0.2 863,753 + +0.2 863,753 + +0.1 863,760 - -0.0 863,801 - -0.1 863,802 + -0.0 863,836 + -0.1 863,847 + -0.1 863,849 - +0.3 863,874 + -0.1 863,915 + +0.0 863,920 + -1.6 863,924 + -0.3 863,969 - -1.1 863,970 + -0.5 863,994 - -0.9 863,995 + -3.5 864,010 - -1.4 864,011 + +0.2 864,047 - -2.2 864,048 + -1.4 864,075 + -0.7 864,245 + -2.2 864,290 + -0.8 864,424 - -2.1 864,425 + -1.3 864,700 - -1.3 864,729 + -2.0 864,763 - -1.2 864,766 - -1.3 864,767 + -1.9 864,771 + +0.1 864,771 + -0.2 864,771 + -1.5
Or see this region's nucleotide sequence