Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00115 and MMP_RS00120 are separated by 72 nucleotides MMP_RS00120 and MMP_RS00125 are separated by 29 nucleotides MMP_RS00125 and MMP_RS00130 are separated by 91 nucleotides MMP_RS00130 and MMP_RS00135 are separated by 116 nucleotides
MMP_RS00115: MMP_RS00115 - radical SAM protein, at 26,673 to 27,725
_RS00115
MMP_RS00120: MMP_RS00120 - LysR family transcriptional regulator, at 27,798 to 28,640
_RS00120
MMP_RS00125: MMP_RS00125 - DUF2798 domain-containing protein, at 28,670 to 28,897
_RS00125
MMP_RS00130: MMP_RS00130 - nickel-responsive transcriptional regulator NikR, at 28,989 to 29,414
_RS00130
MMP_RS00135: MMP_RS00135 - DUF2121 domain-containing protein, at 29,531 to 30,406
_RS00135
Position (kb) 27
28
29 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 26.829 kb on - strand, within MMP_RS00115 at 26.852 kb on - strand, within MMP_RS00115 at 26.855 kb on + strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.856 kb on - strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.857 kb on + strand, within MMP_RS00115 at 26.915 kb on + strand, within MMP_RS00115 at 26.939 kb on - strand, within MMP_RS00115 at 26.940 kb on + strand, within MMP_RS00115 at 26.944 kb on + strand, within MMP_RS00115 at 26.981 kb on - strand, within MMP_RS00115 at 26.987 kb on - strand, within MMP_RS00115 at 27.057 kb on + strand, within MMP_RS00115 at 27.209 kb on - strand, within MMP_RS00115 at 27.387 kb on - strand, within MMP_RS00115 at 27.413 kb on + strand, within MMP_RS00115 at 27.473 kb on - strand, within MMP_RS00115 at 27.492 kb on - strand, within MMP_RS00115 at 27.493 kb on + strand, within MMP_RS00115 at 27.596 kb on - strand, within MMP_RS00115 at 27.614 kb on - strand, within MMP_RS00115 at 27.650 kb on - strand at 27.725 kb on - strand at 27.728 kb on - strand at 27.739 kb on + strand at 27.764 kb on - strand at 27.765 kb on + strand at 27.823 kb on - strand at 27.824 kb on + strand at 27.840 kb on + strand at 27.842 kb on + strand at 27.846 kb on - strand at 27.846 kb on - strand at 27.847 kb on + strand at 27.847 kb on + strand at 27.848 kb on - strand at 28.059 kb on - strand, within MMP_RS00120 at 28.060 kb on + strand, within MMP_RS00120 at 28.071 kb on - strand, within MMP_RS00120 at 28.115 kb on - strand, within MMP_RS00120 at 28.118 kb on + strand, within MMP_RS00120 at 28.332 kb on - strand, within MMP_RS00120 at 28.609 kb on - strand at 28.657 kb on + strand at 28.659 kb on - strand at 28.660 kb on + strand at 28.683 kb on + strand at 28.829 kb on - strand, within MMP_RS00125 at 28.900 kb on + strand at 28.900 kb on + strand at 28.924 kb on - strand at 29.300 kb on - strand, within MMP_RS00130 at 29.390 kb on - strand at 29.390 kb on - strand at 29.390 kb on - strand at 29.417 kb on - strand at 29.417 kb on - strand at 29.418 kb on + strand at 29.453 kb on + strand at 29.459 kb on - strand at 29.516 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 26,829 - MMP_RS00115 0.15 -1.7 26,852 - MMP_RS00115 0.17 -1.4 26,855 + MMP_RS00115 0.17 -1.4 26,856 - MMP_RS00115 0.17 -2.0 26,856 - MMP_RS00115 0.17 -1.9 26,856 - MMP_RS00115 0.17 -2.1 26,856 - MMP_RS00115 0.17 -1.3 26,856 - MMP_RS00115 0.17 -2.3 26,857 + MMP_RS00115 0.17 -2.1 26,857 + MMP_RS00115 0.17 -2.3 26,857 + MMP_RS00115 0.17 -2.0 26,857 + MMP_RS00115 0.17 -2.0 26,915 + MMP_RS00115 0.23 -1.8 26,939 - MMP_RS00115 0.25 -2.1 26,940 + MMP_RS00115 0.25 -1.7 26,944 + MMP_RS00115 0.26 -1.3 26,981 - MMP_RS00115 0.29 -1.1 26,987 - MMP_RS00115 0.30 -1.8 27,057 + MMP_RS00115 0.36 -1.4 27,209 - MMP_RS00115 0.51 -1.4 27,387 - MMP_RS00115 0.68 -1.9 27,413 + MMP_RS00115 0.70 -0.9 27,473 - MMP_RS00115 0.76 -2.0 27,492 - MMP_RS00115 0.78 -1.6 27,493 + MMP_RS00115 0.78 -0.9 27,596 - MMP_RS00115 0.88 -2.9 27,614 - MMP_RS00115 0.89 -2.0 27,650 - -0.3 27,725 - -1.9 27,728 - -1.0 27,739 + -1.8 27,764 - -0.4 27,765 + -0.8 27,823 - -1.4 27,824 + -1.8 27,840 + -2.5 27,842 + -1.3 27,846 - -0.7 27,846 - -0.8 27,847 + -0.7 27,847 + -0.8 27,848 - -1.4 28,059 - MMP_RS00120 0.31 -0.6 28,060 + MMP_RS00120 0.31 -0.6 28,071 - MMP_RS00120 0.32 -0.2 28,115 - MMP_RS00120 0.38 -0.9 28,118 + MMP_RS00120 0.38 -0.6 28,332 - MMP_RS00120 0.63 -0.6 28,609 - -0.7 28,657 + -0.1 28,659 - -0.3 28,660 + +0.1 28,683 + +0.0 28,829 - MMP_RS00125 0.70 +0.1 28,900 + +0.0 28,900 + +0.1 28,924 - +0.6 29,300 - MMP_RS00130 0.73 +0.3 29,390 - -0.2 29,390 - -0.2 29,390 - +0.0 29,417 - -0.5 29,417 - -0.2 29,418 + -0.2 29,453 + -0.8 29,459 - -0.3 29,516 + -0.1
Or see this region's nucleotide sequence