Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15335 and BWI76_RS15340 are separated by 84 nucleotides BWI76_RS15340 and BWI76_RS15345 are separated by 176 nucleotides
BWI76_RS15335: BWI76_RS15335 - branched-chain amino acid ABC transporter permease, at 3,210,917 to 3,212,491
_RS15335
BWI76_RS15340: BWI76_RS15340 - urea ABC transporter substrate-binding protein, at 3,212,576 to 3,213,847
_RS15340
BWI76_RS15345: BWI76_RS15345 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 3,214,024 to 3,215,127
_RS15345
Position (kb)
3212
3213
3214 Strain fitness (log2 ratio)
-2
-1
0
1 at 3211.850 kb on + strand, within BWI76_RS15335 at 3211.885 kb on - strand, within BWI76_RS15335 at 3212.101 kb on + strand, within BWI76_RS15335 at 3212.534 kb on + strand at 3212.758 kb on + strand, within BWI76_RS15340 at 3213.004 kb on - strand, within BWI76_RS15340 at 3213.110 kb on - strand, within BWI76_RS15340 at 3213.476 kb on - strand, within BWI76_RS15340 at 3213.488 kb on - strand, within BWI76_RS15340 at 3213.794 kb on + strand at 3213.795 kb on - strand at 3213.797 kb on + strand at 3213.856 kb on + strand at 3213.856 kb on + strand at 3213.856 kb on + strand at 3213.864 kb on - strand at 3213.864 kb on - strand at 3213.957 kb on - strand at 3214.012 kb on + strand at 3214.033 kb on - strand at 3214.040 kb on - strand at 3214.159 kb on - strand, within BWI76_RS15345 at 3214.451 kb on + strand, within BWI76_RS15345 at 3214.489 kb on + strand, within BWI76_RS15345 at 3214.554 kb on - strand, within BWI76_RS15345 at 3214.844 kb on - strand, within BWI76_RS15345
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 3,211,850 + BWI76_RS15335 0.59 +0.2 3,211,885 - BWI76_RS15335 0.61 +0.7 3,212,101 + BWI76_RS15335 0.75 -0.4 3,212,534 + -0.2 3,212,758 + BWI76_RS15340 0.14 -1.4 3,213,004 - BWI76_RS15340 0.34 -1.4 3,213,110 - BWI76_RS15340 0.42 -0.3 3,213,476 - BWI76_RS15340 0.71 -1.8 3,213,488 - BWI76_RS15340 0.72 -0.0 3,213,794 + +0.8 3,213,795 - +0.3 3,213,797 + +0.5 3,213,856 + +0.9 3,213,856 + -0.1 3,213,856 + -1.0 3,213,864 - +0.1 3,213,864 - -0.9 3,213,957 - -0.2 3,214,012 + +0.4 3,214,033 - -2.6 3,214,040 - -0.1 3,214,159 - BWI76_RS15345 0.12 -0.7 3,214,451 + BWI76_RS15345 0.39 -0.7 3,214,489 + BWI76_RS15345 0.42 -0.4 3,214,554 - BWI76_RS15345 0.48 +0.9 3,214,844 - BWI76_RS15345 0.74 +0.2
Or see this region's nucleotide sequence