Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14195

Experiment: D-Gluconic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14185 and BWI76_RS14190 overlap by 4 nucleotidesBWI76_RS14190 and BWI76_RS14195 are separated by 128 nucleotidesBWI76_RS14195 and BWI76_RS14200 overlap by 4 nucleotides BWI76_RS14185: BWI76_RS14185 - 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase, at 2,969,036 to 2,969,749 _RS14185 BWI76_RS14190: BWI76_RS14190 - DNA-binding protein, at 2,969,746 to 2,970,486 _RS14190 BWI76_RS14195: BWI76_RS14195 - LysR family transcriptional regulator, at 2,970,615 to 2,971,838 _RS14195 BWI76_RS14200: BWI76_RS14200 - protocatechuate 4,5-dioxygenase, at 2,971,835 to 2,973,097 _RS14200 Position (kb) 2970 2971 2972Strain fitness (log2 ratio) -2 -1 0 1at 2969.670 kb on + strand, within BWI76_RS14185at 2969.712 kb on - strandat 2969.992 kb on - strand, within BWI76_RS14190at 2970.301 kb on - strand, within BWI76_RS14190at 2970.346 kb on + strand, within BWI76_RS14190at 2970.382 kb on + strand, within BWI76_RS14190at 2970.393 kb on + strand, within BWI76_RS14190at 2970.395 kb on - strand, within BWI76_RS14190at 2970.395 kb on - strand, within BWI76_RS14190at 2970.399 kb on + strand, within BWI76_RS14190at 2970.402 kb on - strand, within BWI76_RS14190at 2970.402 kb on - strand, within BWI76_RS14190at 2970.402 kb on - strand, within BWI76_RS14190at 2970.407 kb on - strand, within BWI76_RS14190at 2970.438 kb on + strandat 2970.446 kb on - strandat 2970.448 kb on + strandat 2970.477 kb on + strandat 2970.480 kb on - strandat 2970.484 kb on + strandat 2970.484 kb on + strandat 2970.492 kb on - strandat 2970.492 kb on - strandat 2970.495 kb on + strandat 2970.495 kb on + strandat 2970.503 kb on - strandat 2970.518 kb on + strandat 2970.518 kb on + strandat 2970.518 kb on + strandat 2970.518 kb on + strandat 2970.518 kb on + strandat 2970.526 kb on - strandat 2970.526 kb on - strandat 2970.526 kb on - strandat 2970.526 kb on - strandat 2970.538 kb on + strandat 2970.678 kb on + strandat 2970.678 kb on + strandat 2970.685 kb on - strandat 2970.686 kb on + strandat 2970.686 kb on - strandat 2970.687 kb on - strandat 2970.696 kb on - strandat 2970.756 kb on + strand, within BWI76_RS14195at 2970.807 kb on - strand, within BWI76_RS14195at 2970.808 kb on + strand, within BWI76_RS14195at 2970.816 kb on + strand, within BWI76_RS14195at 2970.829 kb on - strand, within BWI76_RS14195at 2970.835 kb on - strand, within BWI76_RS14195at 2970.877 kb on - strand, within BWI76_RS14195at 2970.882 kb on - strand, within BWI76_RS14195at 2970.892 kb on + strand, within BWI76_RS14195at 2970.906 kb on + strand, within BWI76_RS14195at 2970.923 kb on + strand, within BWI76_RS14195at 2970.931 kb on - strand, within BWI76_RS14195at 2970.988 kb on + strand, within BWI76_RS14195at 2971.011 kb on - strand, within BWI76_RS14195at 2971.016 kb on - strand, within BWI76_RS14195at 2971.018 kb on + strand, within BWI76_RS14195at 2971.026 kb on - strand, within BWI76_RS14195at 2971.047 kb on - strand, within BWI76_RS14195at 2971.216 kb on - strand, within BWI76_RS14195at 2971.391 kb on + strand, within BWI76_RS14195at 2971.480 kb on + strand, within BWI76_RS14195at 2971.571 kb on - strand, within BWI76_RS14195at 2971.627 kb on + strand, within BWI76_RS14195at 2972.042 kb on + strand, within BWI76_RS14200at 2972.572 kb on - strand, within BWI76_RS14200at 2972.596 kb on + strand, within BWI76_RS14200at 2972.635 kb on - strand, within BWI76_RS14200

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Gluconic Acid (C)
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2,969,670 + BWI76_RS14185 0.89 +0.0
2,969,712 - +0.3
2,969,992 - BWI76_RS14190 0.33 +1.3
2,970,301 - BWI76_RS14190 0.75 -0.3
2,970,346 + BWI76_RS14190 0.81 -0.4
2,970,382 + BWI76_RS14190 0.86 -1.4
2,970,393 + BWI76_RS14190 0.87 +0.2
2,970,395 - BWI76_RS14190 0.88 +0.8
2,970,395 - BWI76_RS14190 0.88 +1.5
2,970,399 + BWI76_RS14190 0.88 +1.5
2,970,402 - BWI76_RS14190 0.89 -0.5
2,970,402 - BWI76_RS14190 0.89 +0.6
2,970,402 - BWI76_RS14190 0.89 +0.6
2,970,407 - BWI76_RS14190 0.89 +0.5
2,970,438 + -0.1
2,970,446 - -0.9
2,970,448 + +0.2
2,970,477 + +0.5
2,970,480 - -0.1
2,970,484 + -1.7
2,970,484 + -0.7
2,970,492 - -0.5
2,970,492 - +0.5
2,970,495 + +0.4
2,970,495 + +0.1
2,970,503 - +0.4
2,970,518 + +0.8
2,970,518 + -1.5
2,970,518 + +0.3
2,970,518 + -0.7
2,970,518 + +0.7
2,970,526 - -0.2
2,970,526 - -0.1
2,970,526 - -0.3
2,970,526 - -0.0
2,970,538 + -0.5
2,970,678 + +1.5
2,970,678 + -1.6
2,970,685 - +1.0
2,970,686 + -0.1
2,970,686 - +1.1
2,970,687 - -1.4
2,970,696 - +0.9
2,970,756 + BWI76_RS14195 0.12 -0.1
2,970,807 - BWI76_RS14195 0.16 +0.5
2,970,808 + BWI76_RS14195 0.16 -0.8
2,970,816 + BWI76_RS14195 0.16 -0.7
2,970,829 - BWI76_RS14195 0.17 +1.1
2,970,835 - BWI76_RS14195 0.18 +1.0
2,970,877 - BWI76_RS14195 0.21 -0.6
2,970,882 - BWI76_RS14195 0.22 -0.5
2,970,892 + BWI76_RS14195 0.23 +0.2
2,970,906 + BWI76_RS14195 0.24 -2.3
2,970,923 + BWI76_RS14195 0.25 +0.3
2,970,931 - BWI76_RS14195 0.26 -0.9
2,970,988 + BWI76_RS14195 0.30 +0.0
2,971,011 - BWI76_RS14195 0.32 +0.1
2,971,016 - BWI76_RS14195 0.33 -0.9
2,971,018 + BWI76_RS14195 0.33 +0.7
2,971,026 - BWI76_RS14195 0.34 -0.4
2,971,047 - BWI76_RS14195 0.35 +0.6
2,971,216 - BWI76_RS14195 0.49 -0.1
2,971,391 + BWI76_RS14195 0.63 +0.4
2,971,480 + BWI76_RS14195 0.71 -0.8
2,971,571 - BWI76_RS14195 0.78 -0.1
2,971,627 + BWI76_RS14195 0.83 +0.4
2,972,042 + BWI76_RS14200 0.16 -0.4
2,972,572 - BWI76_RS14200 0.58 -0.3
2,972,596 + BWI76_RS14200 0.60 -0.2
2,972,635 - BWI76_RS14200 0.63 -0.9

Or see this region's nucleotide sequence