Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04985

Experiment: D-Gluconic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04980 and BWI76_RS04985 are separated by 1 nucleotidesBWI76_RS04985 and BWI76_RS04990 are separated by 93 nucleotidesBWI76_RS04990 and BWI76_RS04995 are separated by 11 nucleotides BWI76_RS04980: BWI76_RS04980 - hypothetical protein, at 1,045,254 to 1,046,507 _RS04980 BWI76_RS04985: BWI76_RS04985 - aspartate 1-decarboxylase, at 1,046,509 to 1,046,889 _RS04985 BWI76_RS04990: BWI76_RS04990 - pantoate--beta-alanine ligase, at 1,046,983 to 1,047,837 _RS04990 BWI76_RS04995: BWI76_RS04995 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,047,849 to 1,048,640 _RS04995 Position (kb) 1046 1047Strain fitness (log2 ratio) -2 -1 0 1at 1045.514 kb on - strand, within BWI76_RS04980at 1045.691 kb on + strand, within BWI76_RS04980at 1045.699 kb on - strand, within BWI76_RS04980at 1045.904 kb on + strand, within BWI76_RS04980at 1045.912 kb on - strand, within BWI76_RS04980at 1045.912 kb on - strand, within BWI76_RS04980at 1046.015 kb on + strand, within BWI76_RS04980at 1046.017 kb on - strand, within BWI76_RS04980at 1046.260 kb on - strand, within BWI76_RS04980at 1046.501 kb on + strandat 1046.554 kb on + strand, within BWI76_RS04985at 1046.657 kb on - strand, within BWI76_RS04985at 1047.247 kb on - strand, within BWI76_RS04990at 1047.249 kb on + strand, within BWI76_RS04990at 1047.395 kb on + strand, within BWI76_RS04990at 1047.479 kb on + strand, within BWI76_RS04990at 1047.576 kb on - strand, within BWI76_RS04990at 1047.700 kb on + strand, within BWI76_RS04990

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Gluconic Acid (C)
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1,045,514 - BWI76_RS04980 0.21 +0.1
1,045,691 + BWI76_RS04980 0.35 +0.2
1,045,699 - BWI76_RS04980 0.35 -2.0
1,045,904 + BWI76_RS04980 0.52 -1.9
1,045,912 - BWI76_RS04980 0.52 -0.2
1,045,912 - BWI76_RS04980 0.52 -1.1
1,046,015 + BWI76_RS04980 0.61 +0.3
1,046,017 - BWI76_RS04980 0.61 +1.4
1,046,260 - BWI76_RS04980 0.80 -0.3
1,046,501 + -0.0
1,046,554 + BWI76_RS04985 0.12 -0.0
1,046,657 - BWI76_RS04985 0.39 +0.8
1,047,247 - BWI76_RS04990 0.31 +0.8
1,047,249 + BWI76_RS04990 0.31 -0.7
1,047,395 + BWI76_RS04990 0.48 -0.1
1,047,479 + BWI76_RS04990 0.58 +0.3
1,047,576 - BWI76_RS04990 0.69 +0.3
1,047,700 + BWI76_RS04990 0.84 +1.2

Or see this region's nucleotide sequence