Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_673

Experiment: molybdate 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_671 and Pf6N2E2_672 are separated by 51 nucleotidesPf6N2E2_672 and Pf6N2E2_673 overlap by 8 nucleotidesPf6N2E2_673 and Pf6N2E2_674 are separated by 12 nucleotidesPf6N2E2_674 and Pf6N2E2_675 overlap by 8 nucleotides Pf6N2E2_671 - 2-methylaconitate racemase, at 776,215 to 777,243 _671 Pf6N2E2_672 - ABC-type polar amino acid transport system, ATPase component, at 777,295 to 778,017 _672 Pf6N2E2_673 - Probable permease of ABC transporter, at 778,010 to 778,660 _673 Pf6N2E2_674 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 778,673 to 779,338 _674 Pf6N2E2_675 - 2-Methylcitrate dehydratase AcnD, at 779,331 to 781,106 _675 Position (kb) 778 779Strain fitness (log2 ratio) -2 -1 0 1at 777.217 kb on + strandat 777.217 kb on + strandat 777.218 kb on - strandat 777.218 kb on - strandat 777.494 kb on - strand, within Pf6N2E2_672at 777.548 kb on + strand, within Pf6N2E2_672at 777.548 kb on + strand, within Pf6N2E2_672at 777.549 kb on - strand, within Pf6N2E2_672at 778.078 kb on - strand, within Pf6N2E2_673at 778.078 kb on - strand, within Pf6N2E2_673at 778.081 kb on - strand, within Pf6N2E2_673at 778.282 kb on - strand, within Pf6N2E2_673at 778.282 kb on - strand, within Pf6N2E2_673at 778.375 kb on - strand, within Pf6N2E2_673at 778.623 kb on + strandat 778.624 kb on - strandat 778.651 kb on + strandat 778.651 kb on + strandat 778.652 kb on - strandat 778.740 kb on + strand, within Pf6N2E2_674at 778.741 kb on - strand, within Pf6N2E2_674at 778.741 kb on - strand, within Pf6N2E2_674at 778.741 kb on - strand, within Pf6N2E2_674at 778.741 kb on - strand, within Pf6N2E2_674at 778.765 kb on - strand, within Pf6N2E2_674at 778.765 kb on - strand, within Pf6N2E2_674at 778.794 kb on + strand, within Pf6N2E2_674at 778.795 kb on - strand, within Pf6N2E2_674at 778.891 kb on - strand, within Pf6N2E2_674at 778.891 kb on - strand, within Pf6N2E2_674at 779.106 kb on + strand, within Pf6N2E2_674at 779.107 kb on - strand, within Pf6N2E2_674at 779.115 kb on + strand, within Pf6N2E2_674at 779.115 kb on + strand, within Pf6N2E2_674at 779.116 kb on - strand, within Pf6N2E2_674at 779.116 kb on - strand, within Pf6N2E2_674at 779.204 kb on + strand, within Pf6N2E2_674at 779.228 kb on + strand, within Pf6N2E2_674at 779.229 kb on - strand, within Pf6N2E2_674at 779.328 kb on + strandat 779.329 kb on - strandat 779.329 kb on - strandat 779.471 kb on - strandat 779.471 kb on - strandat 779.480 kb on - strandat 779.480 kb on - strandat 779.480 kb on - strandat 779.606 kb on + strand, within Pf6N2E2_675at 779.656 kb on + strand, within Pf6N2E2_675at 779.656 kb on + strand, within Pf6N2E2_675at 779.656 kb on + strand, within Pf6N2E2_675at 779.656 kb on + strand, within Pf6N2E2_675at 779.657 kb on - strand, within Pf6N2E2_675

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Per-strain Table

Position Strand Gene LocusTag Fraction molybdate 0.25 M
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777,217 + -1.2
777,217 + +0.9
777,218 - +1.2
777,218 - -0.1
777,494 - Pf6N2E2_672 0.28 +0.6
777,548 + Pf6N2E2_672 0.35 +0.4
777,548 + Pf6N2E2_672 0.35 -0.0
777,549 - Pf6N2E2_672 0.35 +1.4
778,078 - Pf6N2E2_673 0.10 -2.2
778,078 - Pf6N2E2_673 0.10 -0.2
778,081 - Pf6N2E2_673 0.11 -0.4
778,282 - Pf6N2E2_673 0.42 -1.7
778,282 - Pf6N2E2_673 0.42 +0.3
778,375 - Pf6N2E2_673 0.56 -1.3
778,623 + -0.2
778,624 - -1.2
778,651 + +0.1
778,651 + -1.6
778,652 - +1.6
778,740 + Pf6N2E2_674 0.10 +1.4
778,741 - Pf6N2E2_674 0.10 -0.6
778,741 - Pf6N2E2_674 0.10 -1.2
778,741 - Pf6N2E2_674 0.10 -0.5
778,741 - Pf6N2E2_674 0.10 +0.2
778,765 - Pf6N2E2_674 0.14 -1.8
778,765 - Pf6N2E2_674 0.14 +0.1
778,794 + Pf6N2E2_674 0.18 -0.6
778,795 - Pf6N2E2_674 0.18 -2.1
778,891 - Pf6N2E2_674 0.33 -0.1
778,891 - Pf6N2E2_674 0.33 +0.5
779,106 + Pf6N2E2_674 0.65 -2.2
779,107 - Pf6N2E2_674 0.65 -0.8
779,115 + Pf6N2E2_674 0.66 +1.3
779,115 + Pf6N2E2_674 0.66 +0.3
779,116 - Pf6N2E2_674 0.67 +1.0
779,116 - Pf6N2E2_674 0.67 +0.8
779,204 + Pf6N2E2_674 0.80 -0.7
779,228 + Pf6N2E2_674 0.83 +0.1
779,229 - Pf6N2E2_674 0.83 -0.8
779,328 + +0.8
779,329 - +1.0
779,329 - -0.8
779,471 - -0.6
779,471 - +0.5
779,480 - +1.3
779,480 - -2.2
779,480 - +0.6
779,606 + Pf6N2E2_675 0.15 +0.8
779,656 + Pf6N2E2_675 0.18 -0.2
779,656 + Pf6N2E2_675 0.18 -1.2
779,656 + Pf6N2E2_675 0.18 -0.0
779,656 + Pf6N2E2_675 0.18 -0.0
779,657 - Pf6N2E2_675 0.18 -0.8

Or see this region's nucleotide sequence