Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5936

Experiment: molybdate 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5933 and Pf6N2E2_5934 are separated by 23 nucleotidesPf6N2E2_5934 and Pf6N2E2_5935 overlap by 4 nucleotidesPf6N2E2_5935 and Pf6N2E2_5936 overlap by 1 nucleotidesPf6N2E2_5936 and Pf6N2E2_5937 overlap by 4 nucleotides Pf6N2E2_5933 - Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130), at 6,753,413 to 6,754,423 _5933 Pf6N2E2_5934 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 6,754,447 to 6,754,632 _5934 Pf6N2E2_5935 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 6,754,629 to 6,755,393 _5935 Pf6N2E2_5936 - Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48), at 6,755,393 to 6,755,857 _5936 Pf6N2E2_5937 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 6,755,854 to 6,756,873 _5937 Position (kb) 6755 6756Strain fitness (log2 ratio) -1 0 1at 6754.421 kb on + strandat 6754.431 kb on + strandat 6755.422 kb on + strandat 6755.633 kb on + strand, within Pf6N2E2_5936at 6755.765 kb on + strand, within Pf6N2E2_5936

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Per-strain Table

Position Strand Gene LocusTag Fraction molybdate 0.25 M
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6,754,421 + -0.0
6,754,431 + -1.6
6,755,422 + -0.1
6,755,633 + Pf6N2E2_5936 0.52 -0.3
6,755,765 + Pf6N2E2_5936 0.80 +0.1

Or see this region's nucleotide sequence