Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_52

Experiment: molybdate 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_50 and Pf6N2E2_51 are separated by 15 nucleotidesPf6N2E2_51 and Pf6N2E2_52 are separated by 121 nucleotidesPf6N2E2_52 and Pf6N2E2_53 are separated by 224 nucleotides Pf6N2E2_50 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57), at 88,788 to 89,984 _50 Pf6N2E2_51 - hypothetical protein, at 90,000 to 90,194 _51 Pf6N2E2_52 - Excinuclease ABC subunit B, at 90,316 to 92,331 _52 Pf6N2E2_53 - Glutamyl-tRNA synthetase (EC 6.1.1.17) @ Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24), at 92,556 to 94,037 _53 Position (kb) 90 91 92 93Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 89.530 kb on + strand, within Pf6N2E2_50at 89.531 kb on - strand, within Pf6N2E2_50at 89.758 kb on + strand, within Pf6N2E2_50at 89.759 kb on - strand, within Pf6N2E2_50at 89.786 kb on - strand, within Pf6N2E2_50at 89.786 kb on - strand, within Pf6N2E2_50at 89.786 kb on - strand, within Pf6N2E2_50at 89.786 kb on - strand, within Pf6N2E2_50at 89.852 kb on + strand, within Pf6N2E2_50at 89.852 kb on + strand, within Pf6N2E2_50at 89.853 kb on - strand, within Pf6N2E2_50at 89.853 kb on - strand, within Pf6N2E2_50at 89.872 kb on + strandat 89.872 kb on + strandat 89.873 kb on - strandat 89.903 kb on + strandat 89.985 kb on + strandat 89.986 kb on - strandat 89.998 kb on + strandat 89.999 kb on - strandat 90.065 kb on + strand, within Pf6N2E2_51at 90.065 kb on + strand, within Pf6N2E2_51at 90.065 kb on + strand, within Pf6N2E2_51at 90.065 kb on + strand, within Pf6N2E2_51at 90.065 kb on + strand, within Pf6N2E2_51at 90.065 kb on + strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.066 kb on - strand, within Pf6N2E2_51at 90.068 kb on + strand, within Pf6N2E2_51at 90.068 kb on + strand, within Pf6N2E2_51at 90.068 kb on + strand, within Pf6N2E2_51at 90.068 kb on + strand, within Pf6N2E2_51at 90.069 kb on - strand, within Pf6N2E2_51at 90.069 kb on - strand, within Pf6N2E2_51at 90.070 kb on + strand, within Pf6N2E2_51at 90.070 kb on + strand, within Pf6N2E2_51at 90.070 kb on + strand, within Pf6N2E2_51at 90.070 kb on + strand, within Pf6N2E2_51at 90.071 kb on - strand, within Pf6N2E2_51at 90.178 kb on + strandat 90.178 kb on + strandat 90.310 kb on + strandat 90.310 kb on + strandat 90.310 kb on + strandat 90.311 kb on - strandat 90.332 kb on + strandat 90.332 kb on + strandat 90.333 kb on - strandat 90.532 kb on + strand, within Pf6N2E2_52at 90.532 kb on + strand, within Pf6N2E2_52at 90.574 kb on + strand, within Pf6N2E2_52at 90.574 kb on + strand, within Pf6N2E2_52at 90.575 kb on - strand, within Pf6N2E2_52at 90.586 kb on + strand, within Pf6N2E2_52at 90.587 kb on - strand, within Pf6N2E2_52at 90.595 kb on + strand, within Pf6N2E2_52at 90.595 kb on + strand, within Pf6N2E2_52at 90.598 kb on + strand, within Pf6N2E2_52at 90.599 kb on - strand, within Pf6N2E2_52at 90.599 kb on - strand, within Pf6N2E2_52at 90.599 kb on - strand, within Pf6N2E2_52at 90.613 kb on + strand, within Pf6N2E2_52at 90.614 kb on - strand, within Pf6N2E2_52at 90.614 kb on - strand, within Pf6N2E2_52at 90.748 kb on + strand, within Pf6N2E2_52at 90.748 kb on + strand, within Pf6N2E2_52at 90.749 kb on - strand, within Pf6N2E2_52at 90.772 kb on + strand, within Pf6N2E2_52at 90.773 kb on - strand, within Pf6N2E2_52at 90.773 kb on - strand, within Pf6N2E2_52at 90.773 kb on - strand, within Pf6N2E2_52at 90.853 kb on + strand, within Pf6N2E2_52at 90.853 kb on + strand, within Pf6N2E2_52at 90.853 kb on + strand, within Pf6N2E2_52at 90.854 kb on - strand, within Pf6N2E2_52at 90.854 kb on - strand, within Pf6N2E2_52at 91.043 kb on - strand, within Pf6N2E2_52at 91.057 kb on + strand, within Pf6N2E2_52at 91.058 kb on - strand, within Pf6N2E2_52at 91.058 kb on - strand, within Pf6N2E2_52at 91.129 kb on + strand, within Pf6N2E2_52at 91.130 kb on - strand, within Pf6N2E2_52at 91.217 kb on - strand, within Pf6N2E2_52at 91.237 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.288 kb on + strand, within Pf6N2E2_52at 91.289 kb on - strand, within Pf6N2E2_52at 91.289 kb on - strand, within Pf6N2E2_52at 91.289 kb on - strand, within Pf6N2E2_52at 91.289 kb on - strand, within Pf6N2E2_52at 91.289 kb on - strand, within Pf6N2E2_52at 91.289 kb on - strand, within Pf6N2E2_52at 91.289 kb on - strand, within Pf6N2E2_52at 91.295 kb on - strand, within Pf6N2E2_52at 91.363 kb on + strand, within Pf6N2E2_52at 91.363 kb on + strand, within Pf6N2E2_52at 91.363 kb on + strand, within Pf6N2E2_52at 91.364 kb on - strand, within Pf6N2E2_52at 91.364 kb on - strand, within Pf6N2E2_52at 91.364 kb on - strand, within Pf6N2E2_52at 91.507 kb on + strand, within Pf6N2E2_52at 91.507 kb on + strand, within Pf6N2E2_52at 91.507 kb on + strand, within Pf6N2E2_52at 91.508 kb on - strand, within Pf6N2E2_52at 91.508 kb on - strand, within Pf6N2E2_52at 91.508 kb on - strand, within Pf6N2E2_52at 91.733 kb on - strand, within Pf6N2E2_52at 92.177 kb on - strandat 92.243 kb on - strandat 92.536 kb on + strandat 92.536 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction molybdate 0.25 M
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89,530 + Pf6N2E2_50 0.62 -1.6
89,531 - Pf6N2E2_50 0.62 -1.1
89,758 + Pf6N2E2_50 0.81 +0.0
89,759 - Pf6N2E2_50 0.81 +0.4
89,786 - Pf6N2E2_50 0.83 -2.2
89,786 - Pf6N2E2_50 0.83 +0.1
89,786 - Pf6N2E2_50 0.83 +0.5
89,786 - Pf6N2E2_50 0.83 -2.3
89,852 + Pf6N2E2_50 0.89 +0.7
89,852 + Pf6N2E2_50 0.89 +0.8
89,853 - Pf6N2E2_50 0.89 +0.7
89,853 - Pf6N2E2_50 0.89 -1.1
89,872 + -1.4
89,872 + -0.1
89,873 - +0.7
89,903 + +0.1
89,985 + +0.7
89,986 - -0.1
89,998 + -0.1
89,999 - -0.4
90,065 + Pf6N2E2_51 0.33 +0.3
90,065 + Pf6N2E2_51 0.33 +0.7
90,065 + Pf6N2E2_51 0.33 +0.5
90,065 + Pf6N2E2_51 0.33 +1.0
90,065 + Pf6N2E2_51 0.33 -0.1
90,065 + Pf6N2E2_51 0.33 +0.2
90,066 - Pf6N2E2_51 0.34 -0.2
90,066 - Pf6N2E2_51 0.34 +1.2
90,066 - Pf6N2E2_51 0.34 +0.1
90,066 - Pf6N2E2_51 0.34 -0.1
90,066 - Pf6N2E2_51 0.34 -0.9
90,066 - Pf6N2E2_51 0.34 +0.2
90,066 - Pf6N2E2_51 0.34 -0.1
90,066 - Pf6N2E2_51 0.34 -1.6
90,066 - Pf6N2E2_51 0.34 +0.6
90,066 - Pf6N2E2_51 0.34 -0.3
90,068 + Pf6N2E2_51 0.35 +0.4
90,068 + Pf6N2E2_51 0.35 -0.1
90,068 + Pf6N2E2_51 0.35 +0.1
90,068 + Pf6N2E2_51 0.35 -0.9
90,069 - Pf6N2E2_51 0.35 +0.4
90,069 - Pf6N2E2_51 0.35 +2.5
90,070 + Pf6N2E2_51 0.36 +0.6
90,070 + Pf6N2E2_51 0.36 -1.5
90,070 + Pf6N2E2_51 0.36 +1.4
90,070 + Pf6N2E2_51 0.36 -0.9
90,071 - Pf6N2E2_51 0.36 -0.4
90,178 + +0.2
90,178 + -0.5
90,310 + -0.2
90,310 + -1.9
90,310 + +0.8
90,311 - +0.5
90,332 + -1.5
90,332 + -1.2
90,333 - -0.9
90,532 + Pf6N2E2_52 0.11 -1.3
90,532 + Pf6N2E2_52 0.11 -0.7
90,574 + Pf6N2E2_52 0.13 +0.2
90,574 + Pf6N2E2_52 0.13 -1.2
90,575 - Pf6N2E2_52 0.13 -0.2
90,586 + Pf6N2E2_52 0.13 +0.3
90,587 - Pf6N2E2_52 0.13 -2.2
90,595 + Pf6N2E2_52 0.14 -1.5
90,595 + Pf6N2E2_52 0.14 -1.1
90,598 + Pf6N2E2_52 0.14 -2.6
90,599 - Pf6N2E2_52 0.14 -0.2
90,599 - Pf6N2E2_52 0.14 -0.8
90,599 - Pf6N2E2_52 0.14 -0.1
90,613 + Pf6N2E2_52 0.15 -2.1
90,614 - Pf6N2E2_52 0.15 -1.3
90,614 - Pf6N2E2_52 0.15 +0.5
90,748 + Pf6N2E2_52 0.21 -1.8
90,748 + Pf6N2E2_52 0.21 -0.6
90,749 - Pf6N2E2_52 0.21 -1.3
90,772 + Pf6N2E2_52 0.23 +0.4
90,773 - Pf6N2E2_52 0.23 +0.2
90,773 - Pf6N2E2_52 0.23 -0.1
90,773 - Pf6N2E2_52 0.23 -2.7
90,853 + Pf6N2E2_52 0.27 -0.6
90,853 + Pf6N2E2_52 0.27 -0.7
90,853 + Pf6N2E2_52 0.27 -1.9
90,854 - Pf6N2E2_52 0.27 -1.1
90,854 - Pf6N2E2_52 0.27 -0.9
91,043 - Pf6N2E2_52 0.36 -2.1
91,057 + Pf6N2E2_52 0.37 -0.2
91,058 - Pf6N2E2_52 0.37 -1.1
91,058 - Pf6N2E2_52 0.37 -0.7
91,129 + Pf6N2E2_52 0.40 -2.2
91,130 - Pf6N2E2_52 0.40 -0.8
91,217 - Pf6N2E2_52 0.45 -2.7
91,237 + Pf6N2E2_52 0.46 -0.6
91,288 + Pf6N2E2_52 0.48 -0.4
91,288 + Pf6N2E2_52 0.48 -2.2
91,288 + Pf6N2E2_52 0.48 -3.0
91,288 + Pf6N2E2_52 0.48 -1.6
91,288 + Pf6N2E2_52 0.48 -0.3
91,288 + Pf6N2E2_52 0.48 -0.8
91,288 + Pf6N2E2_52 0.48 -1.8
91,288 + Pf6N2E2_52 0.48 -2.4
91,288 + Pf6N2E2_52 0.48 -0.8
91,288 + Pf6N2E2_52 0.48 -2.9
91,288 + Pf6N2E2_52 0.48 -0.6
91,288 + Pf6N2E2_52 0.48 -0.2
91,288 + Pf6N2E2_52 0.48 -1.6
91,288 + Pf6N2E2_52 0.48 -2.0
91,288 + Pf6N2E2_52 0.48 -0.2
91,288 + Pf6N2E2_52 0.48 -1.3
91,288 + Pf6N2E2_52 0.48 -3.3
91,288 + Pf6N2E2_52 0.48 +0.3
91,289 - Pf6N2E2_52 0.48 -1.0
91,289 - Pf6N2E2_52 0.48 -0.9
91,289 - Pf6N2E2_52 0.48 -0.9
91,289 - Pf6N2E2_52 0.48 +0.1
91,289 - Pf6N2E2_52 0.48 -1.1
91,289 - Pf6N2E2_52 0.48 -2.6
91,289 - Pf6N2E2_52 0.48 -0.5
91,295 - Pf6N2E2_52 0.49 -1.2
91,363 + Pf6N2E2_52 0.52 -0.2
91,363 + Pf6N2E2_52 0.52 -0.5
91,363 + Pf6N2E2_52 0.52 -0.5
91,364 - Pf6N2E2_52 0.52 -1.1
91,364 - Pf6N2E2_52 0.52 -1.6
91,364 - Pf6N2E2_52 0.52 +0.9
91,507 + Pf6N2E2_52 0.59 +0.4
91,507 + Pf6N2E2_52 0.59 -0.8
91,507 + Pf6N2E2_52 0.59 +0.9
91,508 - Pf6N2E2_52 0.59 -2.6
91,508 - Pf6N2E2_52 0.59 -1.0
91,508 - Pf6N2E2_52 0.59 -1.1
91,733 - Pf6N2E2_52 0.70 -2.5
92,177 - +0.3
92,243 - -1.1
92,536 + -1.5
92,536 + +0.2

Or see this region's nucleotide sequence