Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5082

Experiment: molybdate 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5080 and Pf6N2E2_5081 are separated by 140 nucleotidesPf6N2E2_5081 and Pf6N2E2_5082 are separated by 316 nucleotidesPf6N2E2_5082 and Pf6N2E2_5083 are separated by 224 nucleotides Pf6N2E2_5080 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 5,857,079 to 5,858,047 _5080 Pf6N2E2_5081 - hypothetical protein, at 5,858,188 to 5,858,631 _5081 Pf6N2E2_5082 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,858,948 to 5,860,201 _5082 Pf6N2E2_5083 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,860,426 to 5,864,202 _5083 Position (kb) 5858 5859 5860 5861Strain fitness (log2 ratio) -2 -1 0 1 2at 5857.983 kb on + strandat 5857.983 kb on + strandat 5857.983 kb on + strandat 5857.984 kb on - strandat 5857.984 kb on - strandat 5857.984 kb on - strandat 5857.984 kb on - strandat 5858.153 kb on - strandat 5858.200 kb on + strandat 5858.201 kb on - strandat 5858.243 kb on + strand, within Pf6N2E2_5081at 5858.243 kb on + strand, within Pf6N2E2_5081at 5858.269 kb on + strand, within Pf6N2E2_5081at 5858.404 kb on + strand, within Pf6N2E2_5081at 5858.450 kb on + strand, within Pf6N2E2_5081at 5858.450 kb on + strand, within Pf6N2E2_5081at 5858.640 kb on + strandat 5858.723 kb on + strandat 5858.752 kb on - strandat 5858.775 kb on + strandat 5858.803 kb on + strandat 5858.809 kb on + strandat 5858.893 kb on + strandat 5858.930 kb on + strandat 5860.266 kb on + strandat 5860.266 kb on + strandat 5860.267 kb on - strandat 5860.267 kb on - strandat 5860.503 kb on - strandat 5860.706 kb on + strandat 5860.707 kb on - strandat 5860.816 kb on - strand, within Pf6N2E2_5083at 5860.872 kb on + strand, within Pf6N2E2_5083at 5860.873 kb on - strand, within Pf6N2E2_5083at 5861.147 kb on + strand, within Pf6N2E2_5083at 5861.148 kb on - strand, within Pf6N2E2_5083at 5861.150 kb on + strand, within Pf6N2E2_5083at 5861.151 kb on - strand, within Pf6N2E2_5083

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Per-strain Table

Position Strand Gene LocusTag Fraction molybdate 0.25 M
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5,857,983 + +0.2
5,857,983 + +0.1
5,857,983 + -1.2
5,857,984 - +0.1
5,857,984 - +0.4
5,857,984 - +1.0
5,857,984 - +1.5
5,858,153 - +0.0
5,858,200 + -0.6
5,858,201 - +0.0
5,858,243 + Pf6N2E2_5081 0.12 +0.5
5,858,243 + Pf6N2E2_5081 0.12 +0.5
5,858,269 + Pf6N2E2_5081 0.18 +0.3
5,858,404 + Pf6N2E2_5081 0.49 -0.4
5,858,450 + Pf6N2E2_5081 0.59 +1.5
5,858,450 + Pf6N2E2_5081 0.59 +0.1
5,858,640 + +1.9
5,858,723 + +0.7
5,858,752 - -1.4
5,858,775 + -0.3
5,858,803 + -0.3
5,858,809 + -1.0
5,858,893 + -0.7
5,858,930 + +1.0
5,860,266 + +0.3
5,860,266 + +0.6
5,860,267 - +1.3
5,860,267 - +0.7
5,860,503 - +0.1
5,860,706 + -1.9
5,860,707 - -1.2
5,860,816 - Pf6N2E2_5083 0.10 +0.8
5,860,872 + Pf6N2E2_5083 0.12 -0.1
5,860,873 - Pf6N2E2_5083 0.12 -0.3
5,861,147 + Pf6N2E2_5083 0.19 +0.9
5,861,148 - Pf6N2E2_5083 0.19 -2.4
5,861,150 + Pf6N2E2_5083 0.19 -1.6
5,861,151 - Pf6N2E2_5083 0.19 +0.7

Or see this region's nucleotide sequence