Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4599 and Pf6N2E2_4600 are separated by 10 nucleotides Pf6N2E2_4600 and Pf6N2E2_4601 are separated by 35 nucleotides Pf6N2E2_4601 and Pf6N2E2_4602 are separated by 53 nucleotides
Pf6N2E2_4599 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 5,325,694 to 5,326,284
_4599
Pf6N2E2_4600 - Pyrroline-5-carboxylate reductase (EC 1.5.1.2), at 5,326,295 to 5,327,113
_4600
Pf6N2E2_4601 - Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC, at 5,327,149 to 5,327,835
_4601
Pf6N2E2_4602 - Twitching motility protein PilT, at 5,327,889 to 5,328,923
_4602
Position (kb) 5327
5328 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.296 kb on + strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.127 kb on - strand at 5327.127 kb on - strand at 5327.127 kb on - strand at 5327.127 kb on - strand at 5327.663 kb on + strand, within Pf6N2E2_4601 at 5327.664 kb on - strand, within Pf6N2E2_4601 at 5327.664 kb on - strand, within Pf6N2E2_4601 at 5327.803 kb on - strand at 5327.835 kb on + strand at 5327.835 kb on + strand at 5327.835 kb on + strand at 5327.835 kb on + strand at 5327.835 kb on + strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.869 kb on + strand at 5327.905 kb on + strand at 5327.996 kb on + strand, within Pf6N2E2_4602 at 5327.996 kb on + strand, within Pf6N2E2_4602 at 5327.996 kb on + strand, within Pf6N2E2_4602 at 5327.997 kb on - strand, within Pf6N2E2_4602 at 5327.997 kb on - strand, within Pf6N2E2_4602 at 5327.997 kb on - strand, within Pf6N2E2_4602 at 5327.997 kb on - strand, within Pf6N2E2_4602 at 5328.042 kb on + strand, within Pf6N2E2_4602 at 5328.042 kb on + strand, within Pf6N2E2_4602 at 5328.042 kb on + strand, within Pf6N2E2_4602 at 5328.042 kb on + strand, within Pf6N2E2_4602 at 5328.043 kb on - strand, within Pf6N2E2_4602 at 5328.043 kb on - strand, within Pf6N2E2_4602 at 5328.043 kb on - strand, within Pf6N2E2_4602 at 5328.043 kb on - strand, within Pf6N2E2_4602 at 5328.070 kb on + strand, within Pf6N2E2_4602 at 5328.071 kb on - strand, within Pf6N2E2_4602 at 5328.075 kb on + strand, within Pf6N2E2_4602 at 5328.229 kb on + strand, within Pf6N2E2_4602 at 5328.229 kb on + strand, within Pf6N2E2_4602 at 5328.230 kb on - strand, within Pf6N2E2_4602 at 5328.230 kb on - strand, within Pf6N2E2_4602 at 5328.230 kb on - strand, within Pf6N2E2_4602 at 5328.325 kb on - strand, within Pf6N2E2_4602 at 5328.792 kb on + strand, within Pf6N2E2_4602 at 5328.792 kb on + strand, within Pf6N2E2_4602 at 5328.792 kb on + strand, within Pf6N2E2_4602 at 5328.792 kb on + strand, within Pf6N2E2_4602 at 5328.792 kb on + strand, within Pf6N2E2_4602 at 5328.793 kb on - strand, within Pf6N2E2_4602 at 5328.793 kb on - strand, within Pf6N2E2_4602
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 5,326,175 + Pf6N2E2_4599 0.81 -0.2 5,326,175 + Pf6N2E2_4599 0.81 +0.5 5,326,175 + Pf6N2E2_4599 0.81 +1.3 5,326,175 + Pf6N2E2_4599 0.81 -0.2 5,326,288 + +2.3 5,326,288 + +0.5 5,326,288 + +0.8 5,326,288 + -0.7 5,326,289 - +0.9 5,326,289 - +0.2 5,326,289 - +0.5 5,326,289 - -1.4 5,326,296 + -0.3 5,327,125 - +0.1 5,327,125 - -1.3 5,327,125 - +0.7 5,327,125 - -1.6 5,327,125 - -0.3 5,327,127 - -1.8 5,327,127 - -1.3 5,327,127 - +1.1 5,327,127 - -0.9 5,327,663 + Pf6N2E2_4601 0.75 -0.0 5,327,664 - Pf6N2E2_4601 0.75 -0.9 5,327,664 - Pf6N2E2_4601 0.75 -1.0 5,327,803 - -0.9 5,327,835 + -1.8 5,327,835 + +1.8 5,327,835 + -0.3 5,327,835 + +0.8 5,327,835 + +1.3 5,327,836 - -0.9 5,327,836 - -0.3 5,327,836 - -1.2 5,327,836 - +1.9 5,327,836 - -0.3 5,327,836 - +0.3 5,327,836 - -0.6 5,327,869 + +0.4 5,327,905 + +0.4 5,327,996 + Pf6N2E2_4602 0.10 +2.3 5,327,996 + Pf6N2E2_4602 0.10 -0.1 5,327,996 + Pf6N2E2_4602 0.10 +1.0 5,327,997 - Pf6N2E2_4602 0.10 +1.7 5,327,997 - Pf6N2E2_4602 0.10 -1.7 5,327,997 - Pf6N2E2_4602 0.10 +1.1 5,327,997 - Pf6N2E2_4602 0.10 -0.2 5,328,042 + Pf6N2E2_4602 0.15 -1.3 5,328,042 + Pf6N2E2_4602 0.15 +0.6 5,328,042 + Pf6N2E2_4602 0.15 -0.1 5,328,042 + Pf6N2E2_4602 0.15 -0.3 5,328,043 - Pf6N2E2_4602 0.15 +0.7 5,328,043 - Pf6N2E2_4602 0.15 -0.9 5,328,043 - Pf6N2E2_4602 0.15 +0.0 5,328,043 - Pf6N2E2_4602 0.15 -0.7 5,328,070 + Pf6N2E2_4602 0.17 -0.6 5,328,071 - Pf6N2E2_4602 0.18 -0.6 5,328,075 + Pf6N2E2_4602 0.18 -1.5 5,328,229 + Pf6N2E2_4602 0.33 +0.8 5,328,229 + Pf6N2E2_4602 0.33 -2.2 5,328,230 - Pf6N2E2_4602 0.33 +0.7 5,328,230 - Pf6N2E2_4602 0.33 -0.5 5,328,230 - Pf6N2E2_4602 0.33 -1.1 5,328,325 - Pf6N2E2_4602 0.42 -0.0 5,328,792 + Pf6N2E2_4602 0.87 +0.2 5,328,792 + Pf6N2E2_4602 0.87 -0.1 5,328,792 + Pf6N2E2_4602 0.87 -0.7 5,328,792 + Pf6N2E2_4602 0.87 +0.9 5,328,792 + Pf6N2E2_4602 0.87 +0.8 5,328,793 - Pf6N2E2_4602 0.87 -0.4 5,328,793 - Pf6N2E2_4602 0.87 -1.2
Or see this region's nucleotide sequence