Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2382 and Pf6N2E2_2383 are separated by 108 nucleotides Pf6N2E2_2383 and Pf6N2E2_2384 are separated by 295 nucleotides Pf6N2E2_2384 and Pf6N2E2_2385 are separated by 2 nucleotides Pf6N2E2_2385 and Pf6N2E2_2386 are separated by 63 nucleotides
Pf6N2E2_2382 - Major porin and structural outer membrane porin OprF, at 2,821,381 to 2,822,415
_2382
Pf6N2E2_2383 - DNA-directed RNA polymerase specialized sigma subunit, sigma24-like, at 2,822,524 to 2,822,910
_2383
Pf6N2E2_2384 - CmpX, at 2,823,206 to 2,824,030
_2384
Pf6N2E2_2385 - CrfX protein, at 2,824,033 to 2,824,278
_2385
Pf6N2E2_2386 - Magnesium and cobalt transport protein CorA, at 2,824,342 to 2,825,337
_2386
Position (kb)
2823
2824
2825 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2822.498 kb on - strand at 2822.504 kb on - strand at 2822.504 kb on - strand at 2823.095 kb on - strand at 2823.136 kb on - strand at 2823.185 kb on - strand at 2823.185 kb on - strand at 2823.185 kb on - strand at 2823.185 kb on - strand at 2823.185 kb on - strand at 2823.355 kb on - strand, within Pf6N2E2_2384 at 2823.370 kb on - strand, within Pf6N2E2_2384 at 2823.370 kb on - strand, within Pf6N2E2_2384 at 2823.370 kb on - strand, within Pf6N2E2_2384 at 2823.518 kb on - strand, within Pf6N2E2_2384 at 2823.518 kb on - strand, within Pf6N2E2_2384 at 2823.518 kb on - strand, within Pf6N2E2_2384 at 2823.689 kb on - strand, within Pf6N2E2_2384 at 2823.756 kb on - strand, within Pf6N2E2_2384 at 2823.756 kb on - strand, within Pf6N2E2_2384 at 2823.756 kb on - strand, within Pf6N2E2_2384 at 2823.965 kb on - strand at 2823.965 kb on - strand at 2823.969 kb on - strand at 2824.030 kb on + strand at 2824.031 kb on - strand at 2824.045 kb on - strand at 2824.060 kb on - strand, within Pf6N2E2_2385 at 2824.523 kb on + strand, within Pf6N2E2_2386 at 2824.523 kb on + strand, within Pf6N2E2_2386 at 2824.524 kb on - strand, within Pf6N2E2_2386 at 2824.650 kb on - strand, within Pf6N2E2_2386 at 2824.787 kb on + strand, within Pf6N2E2_2386 at 2824.787 kb on + strand, within Pf6N2E2_2386 at 2824.788 kb on - strand, within Pf6N2E2_2386 at 2824.788 kb on - strand, within Pf6N2E2_2386 at 2824.788 kb on - strand, within Pf6N2E2_2386 at 2824.886 kb on + strand, within Pf6N2E2_2386 at 2824.887 kb on - strand, within Pf6N2E2_2386
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 2,822,498 - +1.6 2,822,504 - +1.9 2,822,504 - +1.2 2,823,095 - +1.4 2,823,136 - +1.0 2,823,185 - +1.3 2,823,185 - +0.4 2,823,185 - +1.8 2,823,185 - +0.8 2,823,185 - +2.4 2,823,355 - Pf6N2E2_2384 0.18 -0.4 2,823,370 - Pf6N2E2_2384 0.20 -2.0 2,823,370 - Pf6N2E2_2384 0.20 +1.6 2,823,370 - Pf6N2E2_2384 0.20 -0.4 2,823,518 - Pf6N2E2_2384 0.38 +1.6 2,823,518 - Pf6N2E2_2384 0.38 -1.8 2,823,518 - Pf6N2E2_2384 0.38 -2.2 2,823,689 - Pf6N2E2_2384 0.59 -2.2 2,823,756 - Pf6N2E2_2384 0.67 -2.2 2,823,756 - Pf6N2E2_2384 0.67 +0.2 2,823,756 - Pf6N2E2_2384 0.67 +0.5 2,823,965 - -0.5 2,823,965 - -0.7 2,823,969 - +0.7 2,824,030 + +0.6 2,824,031 - -0.2 2,824,045 - +0.5 2,824,060 - Pf6N2E2_2385 0.11 -2.4 2,824,523 + Pf6N2E2_2386 0.18 -0.0 2,824,523 + Pf6N2E2_2386 0.18 +1.1 2,824,524 - Pf6N2E2_2386 0.18 +0.9 2,824,650 - Pf6N2E2_2386 0.31 +2.6 2,824,787 + Pf6N2E2_2386 0.45 -0.2 2,824,787 + Pf6N2E2_2386 0.45 +0.9 2,824,788 - Pf6N2E2_2386 0.45 +0.4 2,824,788 - Pf6N2E2_2386 0.45 -1.0 2,824,788 - Pf6N2E2_2386 0.45 -0.2 2,824,886 + Pf6N2E2_2386 0.55 +1.5 2,824,887 - Pf6N2E2_2386 0.55 +2.8
Or see this region's nucleotide sequence