Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2310 and Pf6N2E2_2311 overlap by 1 nucleotides Pf6N2E2_2311 and Pf6N2E2_2312 are separated by 18 nucleotides Pf6N2E2_2312 and Pf6N2E2_2313 overlap by 4 nucleotides Pf6N2E2_2313 and Pf6N2E2_2314 are separated by 147 nucleotides Pf6N2E2_2314 and Pf6N2E2_2315 overlap by 1 nucleotides
Pf6N2E2_2310 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 2,734,242 to 2,735,684
_2310
Pf6N2E2_2311 - Cytochrome c oxidase subunit CcoO (EC 1.9.3.1), at 2,735,684 to 2,736,292
_2311
Pf6N2E2_2312 - Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1), at 2,736,311 to 2,736,484
_2312
Pf6N2E2_2313 - Cytochrome c oxidase subunit CcoP (EC 1.9.3.1), at 2,736,481 to 2,737,464
_2313
Pf6N2E2_2314 - VapB protein (antitoxin to VapC), at 2,737,612 to 2,737,866
_2314
Pf6N2E2_2315 - VapC toxin protein, at 2,737,866 to 2,738,327
_2315
Position (kb)
2736
2737
2738 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2735.505 kb on + strand, within Pf6N2E2_2310 at 2735.505 kb on + strand, within Pf6N2E2_2310 at 2735.506 kb on - strand, within Pf6N2E2_2310 at 2735.601 kb on + strand at 2735.601 kb on + strand at 2735.602 kb on - strand at 2735.602 kb on - strand at 2735.611 kb on - strand at 2735.669 kb on - strand at 2735.701 kb on - strand at 2735.797 kb on + strand, within Pf6N2E2_2311 at 2735.798 kb on - strand, within Pf6N2E2_2311 at 2735.858 kb on + strand at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.858 kb on + strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.859 kb on - strand, within Pf6N2E2_2311 at 2735.927 kb on + strand, within Pf6N2E2_2311 at 2735.927 kb on + strand, within Pf6N2E2_2311 at 2735.937 kb on - strand, within Pf6N2E2_2311 at 2735.992 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.056 kb on + strand, within Pf6N2E2_2311 at 2736.057 kb on - strand, within Pf6N2E2_2311 at 2736.179 kb on + strand, within Pf6N2E2_2311 at 2736.466 kb on + strand, within Pf6N2E2_2312 at 2736.466 kb on + strand, within Pf6N2E2_2312 at 2736.466 kb on + strand, within Pf6N2E2_2312 at 2736.628 kb on + strand, within Pf6N2E2_2313 at 2736.745 kb on + strand, within Pf6N2E2_2313 at 2736.745 kb on + strand, within Pf6N2E2_2313 at 2736.751 kb on + strand, within Pf6N2E2_2313 at 2736.752 kb on - strand, within Pf6N2E2_2313 at 2737.301 kb on - strand, within Pf6N2E2_2313 at 2737.301 kb on - strand, within Pf6N2E2_2313 at 2737.411 kb on + strand at 2737.411 kb on + strand at 2737.418 kb on - strand at 2737.418 kb on - strand at 2737.480 kb on + strand at 2737.549 kb on - strand at 2737.549 kb on - strand at 2737.573 kb on - strand at 2737.576 kb on - strand at 2737.584 kb on + strand at 2737.626 kb on + strand at 2737.627 kb on - strand at 2737.731 kb on - strand, within Pf6N2E2_2314 at 2737.745 kb on - strand, within Pf6N2E2_2314 at 2737.745 kb on - strand, within Pf6N2E2_2314 at 2737.869 kb on + strand at 2737.869 kb on + strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.870 kb on - strand at 2737.883 kb on + strand at 2737.884 kb on - strand at 2737.884 kb on - strand at 2737.884 kb on - strand at 2737.927 kb on + strand, within Pf6N2E2_2315 at 2737.928 kb on - strand, within Pf6N2E2_2315 at 2737.965 kb on + strand, within Pf6N2E2_2315 at 2737.966 kb on - strand, within Pf6N2E2_2315 at 2737.966 kb on - strand, within Pf6N2E2_2315 at 2737.979 kb on + strand, within Pf6N2E2_2315 at 2737.980 kb on - strand, within Pf6N2E2_2315 at 2737.984 kb on + strand, within Pf6N2E2_2315 at 2738.006 kb on - strand, within Pf6N2E2_2315 at 2738.210 kb on - strand, within Pf6N2E2_2315 at 2738.325 kb on + strand at 2738.326 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 2,735,505 + Pf6N2E2_2310 0.88 -0.8 2,735,505 + Pf6N2E2_2310 0.88 -0.0 2,735,506 - Pf6N2E2_2310 0.88 +0.8 2,735,601 + -1.9 2,735,601 + -1.4 2,735,602 - -0.4 2,735,602 - -0.2 2,735,611 - -1.5 2,735,669 - -0.8 2,735,701 - +0.6 2,735,797 + Pf6N2E2_2311 0.19 -3.0 2,735,798 - Pf6N2E2_2311 0.19 -0.8 2,735,858 + +0.6 2,735,858 + Pf6N2E2_2311 0.29 -0.9 2,735,858 + Pf6N2E2_2311 0.29 -0.8 2,735,858 + Pf6N2E2_2311 0.29 -1.4 2,735,858 + Pf6N2E2_2311 0.29 -1.1 2,735,858 + Pf6N2E2_2311 0.29 -1.3 2,735,858 + Pf6N2E2_2311 0.29 -1.6 2,735,858 + Pf6N2E2_2311 0.29 +0.1 2,735,858 + Pf6N2E2_2311 0.29 -1.8 2,735,858 + Pf6N2E2_2311 0.29 -2.2 2,735,858 + Pf6N2E2_2311 0.29 -1.4 2,735,858 + Pf6N2E2_2311 0.29 -1.1 2,735,858 + Pf6N2E2_2311 0.29 -1.4 2,735,859 - Pf6N2E2_2311 0.29 -0.4 2,735,859 - Pf6N2E2_2311 0.29 -1.2 2,735,859 - Pf6N2E2_2311 0.29 -3.1 2,735,859 - -0.0 2,735,859 - Pf6N2E2_2311 0.29 -0.0 2,735,859 - Pf6N2E2_2311 0.29 -1.5 2,735,927 + Pf6N2E2_2311 0.40 -1.0 2,735,927 + Pf6N2E2_2311 0.40 -1.2 2,735,937 - Pf6N2E2_2311 0.42 -2.4 2,735,992 + Pf6N2E2_2311 0.51 -0.4 2,736,056 + Pf6N2E2_2311 0.61 -0.6 2,736,056 + Pf6N2E2_2311 0.61 -1.9 2,736,056 + Pf6N2E2_2311 0.61 -1.8 2,736,056 + Pf6N2E2_2311 0.61 +1.6 2,736,056 + Pf6N2E2_2311 0.61 -1.2 2,736,056 + Pf6N2E2_2311 0.61 -1.1 2,736,057 - Pf6N2E2_2311 0.61 -2.6 2,736,179 + Pf6N2E2_2311 0.81 -1.1 2,736,466 + Pf6N2E2_2312 0.89 -1.2 2,736,466 + Pf6N2E2_2312 0.89 -0.2 2,736,466 + Pf6N2E2_2312 0.89 -0.2 2,736,628 + Pf6N2E2_2313 0.15 -0.7 2,736,745 + Pf6N2E2_2313 0.27 -2.1 2,736,745 + Pf6N2E2_2313 0.27 +0.3 2,736,751 + Pf6N2E2_2313 0.27 -2.1 2,736,752 - Pf6N2E2_2313 0.28 -1.0 2,737,301 - Pf6N2E2_2313 0.83 -1.2 2,737,301 - Pf6N2E2_2313 0.83 +0.6 2,737,411 + +0.8 2,737,411 + -1.6 2,737,418 - -0.4 2,737,418 - +0.6 2,737,480 + -1.0 2,737,549 - -1.6 2,737,549 - +0.3 2,737,573 - +1.6 2,737,576 - -0.4 2,737,584 + +0.9 2,737,626 + +0.7 2,737,627 - +0.5 2,737,731 - Pf6N2E2_2314 0.47 +1.4 2,737,745 - Pf6N2E2_2314 0.52 -0.9 2,737,745 - Pf6N2E2_2314 0.52 +0.5 2,737,869 + +0.8 2,737,869 + -0.4 2,737,870 - +0.9 2,737,870 - +0.5 2,737,870 - +0.1 2,737,870 - +0.2 2,737,870 - +0.2 2,737,870 - -1.5 2,737,870 - +0.4 2,737,883 + +1.2 2,737,884 - -0.5 2,737,884 - -2.3 2,737,884 - -0.7 2,737,927 + Pf6N2E2_2315 0.13 -0.0 2,737,928 - Pf6N2E2_2315 0.13 -1.0 2,737,965 + Pf6N2E2_2315 0.21 -0.6 2,737,966 - Pf6N2E2_2315 0.22 +0.7 2,737,966 - Pf6N2E2_2315 0.22 +0.3 2,737,979 + Pf6N2E2_2315 0.24 -0.4 2,737,980 - Pf6N2E2_2315 0.25 -0.1 2,737,984 + Pf6N2E2_2315 0.26 +0.2 2,738,006 - Pf6N2E2_2315 0.30 -0.4 2,738,210 - Pf6N2E2_2315 0.74 -1.0 2,738,325 + -0.0 2,738,326 - +0.3
Or see this region's nucleotide sequence