Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2116 and Pf6N2E2_2117 are separated by 196 nucleotides Pf6N2E2_2117 and Pf6N2E2_2118 are separated by 11 nucleotides
Pf6N2E2_2116 - Glucarate dehydratase (EC 4.2.1.40), at 2,513,809 to 2,515,152
_2116
Pf6N2E2_2117 - 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18), at 2,515,349 to 2,517,583
_2117
Pf6N2E2_2118 - Trehalose synthase (EC 5.4.99.16), at 2,517,595 to 2,520,939
_2118
Position (kb)
2515
2516
2517
2518 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2514.578 kb on + strand, within Pf6N2E2_2116 at 2514.578 kb on + strand, within Pf6N2E2_2116 at 2514.579 kb on - strand, within Pf6N2E2_2116 at 2514.587 kb on - strand, within Pf6N2E2_2116 at 2515.051 kb on + strand at 2515.051 kb on + strand at 2515.051 kb on + strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.052 kb on - strand at 2515.068 kb on - strand at 2515.208 kb on - strand at 2515.208 kb on - strand at 2515.343 kb on + strand at 2515.350 kb on + strand at 2515.461 kb on + strand at 2515.462 kb on - strand at 2515.462 kb on - strand at 2515.462 kb on - strand at 2515.476 kb on + strand at 2515.476 kb on + strand at 2515.477 kb on - strand at 2515.608 kb on - strand, within Pf6N2E2_2117 at 2515.617 kb on + strand, within Pf6N2E2_2117 at 2515.618 kb on - strand, within Pf6N2E2_2117 at 2515.768 kb on - strand, within Pf6N2E2_2117 at 2515.869 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.989 kb on + strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2515.990 kb on - strand, within Pf6N2E2_2117 at 2516.052 kb on + strand, within Pf6N2E2_2117 at 2516.052 kb on + strand, within Pf6N2E2_2117 at 2516.053 kb on - strand, within Pf6N2E2_2117 at 2516.053 kb on - strand, within Pf6N2E2_2117 at 2516.053 kb on - strand, within Pf6N2E2_2117 at 2516.091 kb on + strand, within Pf6N2E2_2117 at 2516.092 kb on - strand, within Pf6N2E2_2117 at 2516.092 kb on - strand, within Pf6N2E2_2117 at 2516.092 kb on - strand, within Pf6N2E2_2117 at 2516.254 kb on + strand, within Pf6N2E2_2117 at 2516.255 kb on - strand, within Pf6N2E2_2117 at 2516.292 kb on + strand, within Pf6N2E2_2117 at 2516.403 kb on + strand, within Pf6N2E2_2117 at 2516.451 kb on + strand, within Pf6N2E2_2117 at 2516.458 kb on - strand, within Pf6N2E2_2117 at 2516.704 kb on - strand, within Pf6N2E2_2117 at 2516.704 kb on - strand, within Pf6N2E2_2117 at 2516.739 kb on + strand, within Pf6N2E2_2117 at 2516.739 kb on + strand, within Pf6N2E2_2117 at 2516.739 kb on + strand, within Pf6N2E2_2117 at 2516.740 kb on - strand, within Pf6N2E2_2117 at 2516.740 kb on - strand, within Pf6N2E2_2117 at 2516.802 kb on + strand, within Pf6N2E2_2117 at 2516.802 kb on + strand, within Pf6N2E2_2117 at 2516.982 kb on + strand at 2516.983 kb on - strand, within Pf6N2E2_2117 at 2516.983 kb on - strand, within Pf6N2E2_2117 at 2516.988 kb on + strand, within Pf6N2E2_2117 at 2516.988 kb on + strand, within Pf6N2E2_2117 at 2516.989 kb on - strand, within Pf6N2E2_2117 at 2516.989 kb on - strand, within Pf6N2E2_2117 at 2517.020 kb on + strand, within Pf6N2E2_2117 at 2517.020 kb on + strand, within Pf6N2E2_2117 at 2517.020 kb on + strand, within Pf6N2E2_2117 at 2517.021 kb on - strand, within Pf6N2E2_2117 at 2517.021 kb on - strand, within Pf6N2E2_2117 at 2517.021 kb on - strand, within Pf6N2E2_2117 at 2517.021 kb on - strand, within Pf6N2E2_2117 at 2517.021 kb on - strand, within Pf6N2E2_2117 at 2517.219 kb on + strand, within Pf6N2E2_2117 at 2517.219 kb on + strand, within Pf6N2E2_2117 at 2517.220 kb on - strand, within Pf6N2E2_2117 at 2517.255 kb on + strand, within Pf6N2E2_2117 at 2517.255 kb on + strand, within Pf6N2E2_2117 at 2517.306 kb on + strand, within Pf6N2E2_2117 at 2517.306 kb on + strand, within Pf6N2E2_2117 at 2517.306 kb on + strand, within Pf6N2E2_2117 at 2517.306 kb on + strand, within Pf6N2E2_2117 at 2517.307 kb on - strand, within Pf6N2E2_2117 at 2517.307 kb on - strand, within Pf6N2E2_2117 at 2517.699 kb on - strand at 2517.711 kb on - strand at 2517.786 kb on + strand at 2517.786 kb on + strand at 2517.787 kb on - strand at 2517.804 kb on - strand at 2517.923 kb on + strand at 2517.924 kb on - strand at 2517.924 kb on - strand at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.957 kb on + strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.958 kb on - strand, within Pf6N2E2_2118 at 2517.959 kb on + strand, within Pf6N2E2_2118 at 2517.959 kb on + strand, within Pf6N2E2_2118 at 2517.959 kb on + strand, within Pf6N2E2_2118 at 2517.960 kb on - strand, within Pf6N2E2_2118 at 2517.960 kb on - strand, within Pf6N2E2_2118 at 2518.049 kb on - strand, within Pf6N2E2_2118 at 2518.321 kb on + strand, within Pf6N2E2_2118 at 2518.391 kb on + strand, within Pf6N2E2_2118 at 2518.391 kb on + strand, within Pf6N2E2_2118 at 2518.392 kb on - strand, within Pf6N2E2_2118 at 2518.493 kb on + strand, within Pf6N2E2_2118 at 2518.494 kb on - strand, within Pf6N2E2_2118
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 2,514,578 + Pf6N2E2_2116 0.57 -1.2 2,514,578 + Pf6N2E2_2116 0.57 -0.3 2,514,579 - Pf6N2E2_2116 0.57 -0.9 2,514,587 - Pf6N2E2_2116 0.58 -0.3 2,515,051 + +0.8 2,515,051 + +0.4 2,515,051 + +0.5 2,515,052 - +1.1 2,515,052 - +0.2 2,515,052 - +0.4 2,515,052 - +0.4 2,515,052 - -0.0 2,515,052 - -1.0 2,515,052 - +0.5 2,515,068 - -0.4 2,515,208 - -0.0 2,515,208 - +0.3 2,515,343 + -1.3 2,515,350 + -1.2 2,515,461 + -1.5 2,515,462 - -0.5 2,515,462 - -0.5 2,515,462 - -2.7 2,515,476 + +0.4 2,515,476 + -1.3 2,515,477 - -1.2 2,515,608 - Pf6N2E2_2117 0.12 -2.3 2,515,617 + Pf6N2E2_2117 0.12 +0.2 2,515,618 - Pf6N2E2_2117 0.12 +0.3 2,515,768 - Pf6N2E2_2117 0.19 -0.7 2,515,869 + Pf6N2E2_2117 0.23 -3.0 2,515,989 + Pf6N2E2_2117 0.29 -0.5 2,515,989 + Pf6N2E2_2117 0.29 -1.9 2,515,989 + Pf6N2E2_2117 0.29 -0.2 2,515,989 + Pf6N2E2_2117 0.29 -0.3 2,515,989 + Pf6N2E2_2117 0.29 -2.5 2,515,989 + Pf6N2E2_2117 0.29 -0.6 2,515,989 + Pf6N2E2_2117 0.29 -1.5 2,515,989 + Pf6N2E2_2117 0.29 -1.4 2,515,990 - Pf6N2E2_2117 0.29 -1.0 2,515,990 - Pf6N2E2_2117 0.29 +0.1 2,515,990 - Pf6N2E2_2117 0.29 -0.5 2,515,990 - Pf6N2E2_2117 0.29 +0.1 2,515,990 - Pf6N2E2_2117 0.29 -0.8 2,515,990 - Pf6N2E2_2117 0.29 -0.7 2,515,990 - Pf6N2E2_2117 0.29 -0.4 2,515,990 - Pf6N2E2_2117 0.29 -1.9 2,515,990 - Pf6N2E2_2117 0.29 -1.5 2,516,052 + Pf6N2E2_2117 0.31 -0.5 2,516,052 + Pf6N2E2_2117 0.31 -1.0 2,516,053 - Pf6N2E2_2117 0.31 -0.5 2,516,053 - Pf6N2E2_2117 0.31 -2.0 2,516,053 - Pf6N2E2_2117 0.31 -1.0 2,516,091 + Pf6N2E2_2117 0.33 -0.5 2,516,092 - Pf6N2E2_2117 0.33 -2.2 2,516,092 - Pf6N2E2_2117 0.33 -1.2 2,516,092 - Pf6N2E2_2117 0.33 -1.2 2,516,254 + Pf6N2E2_2117 0.40 -1.2 2,516,255 - Pf6N2E2_2117 0.41 -1.8 2,516,292 + Pf6N2E2_2117 0.42 -0.7 2,516,403 + Pf6N2E2_2117 0.47 -0.7 2,516,451 + Pf6N2E2_2117 0.49 -0.8 2,516,458 - Pf6N2E2_2117 0.50 +0.5 2,516,704 - Pf6N2E2_2117 0.61 -0.8 2,516,704 - Pf6N2E2_2117 0.61 -1.3 2,516,739 + Pf6N2E2_2117 0.62 +0.3 2,516,739 + Pf6N2E2_2117 0.62 -0.7 2,516,739 + Pf6N2E2_2117 0.62 -0.8 2,516,740 - Pf6N2E2_2117 0.62 -3.3 2,516,740 - Pf6N2E2_2117 0.62 -0.3 2,516,802 + Pf6N2E2_2117 0.65 -1.0 2,516,802 + Pf6N2E2_2117 0.65 -2.3 2,516,982 + +0.5 2,516,983 - Pf6N2E2_2117 0.73 -0.0 2,516,983 - Pf6N2E2_2117 0.73 -1.6 2,516,988 + Pf6N2E2_2117 0.73 -0.6 2,516,988 + Pf6N2E2_2117 0.73 -1.9 2,516,989 - Pf6N2E2_2117 0.73 +0.2 2,516,989 - Pf6N2E2_2117 0.73 -1.5 2,517,020 + Pf6N2E2_2117 0.75 -0.3 2,517,020 + Pf6N2E2_2117 0.75 +0.5 2,517,020 + Pf6N2E2_2117 0.75 -1.3 2,517,021 - Pf6N2E2_2117 0.75 +0.5 2,517,021 - Pf6N2E2_2117 0.75 -2.4 2,517,021 - Pf6N2E2_2117 0.75 -0.0 2,517,021 - Pf6N2E2_2117 0.75 -1.5 2,517,021 - Pf6N2E2_2117 0.75 -1.4 2,517,219 + Pf6N2E2_2117 0.84 -1.2 2,517,219 + Pf6N2E2_2117 0.84 -1.8 2,517,220 - Pf6N2E2_2117 0.84 -0.5 2,517,255 + Pf6N2E2_2117 0.85 +0.2 2,517,255 + Pf6N2E2_2117 0.85 -1.5 2,517,306 + Pf6N2E2_2117 0.88 -2.8 2,517,306 + Pf6N2E2_2117 0.88 -1.0 2,517,306 + Pf6N2E2_2117 0.88 -1.6 2,517,306 + Pf6N2E2_2117 0.88 -0.6 2,517,307 - Pf6N2E2_2117 0.88 -0.0 2,517,307 - Pf6N2E2_2117 0.88 -0.2 2,517,699 - +0.4 2,517,711 - +1.3 2,517,786 + +0.8 2,517,786 + -0.6 2,517,787 - +1.8 2,517,804 - +1.8 2,517,923 + +0.0 2,517,924 - +1.1 2,517,924 - +0.5 2,517,957 + Pf6N2E2_2118 0.11 +0.7 2,517,957 + Pf6N2E2_2118 0.11 +0.2 2,517,957 + Pf6N2E2_2118 0.11 -1.3 2,517,957 + Pf6N2E2_2118 0.11 +0.1 2,517,957 + Pf6N2E2_2118 0.11 +0.0 2,517,957 + Pf6N2E2_2118 0.11 -2.2 2,517,957 + Pf6N2E2_2118 0.11 -0.3 2,517,957 + Pf6N2E2_2118 0.11 +0.8 2,517,957 + Pf6N2E2_2118 0.11 +0.3 2,517,958 - Pf6N2E2_2118 0.11 +1.7 2,517,958 - Pf6N2E2_2118 0.11 +0.0 2,517,958 - Pf6N2E2_2118 0.11 +1.0 2,517,958 - Pf6N2E2_2118 0.11 -0.5 2,517,958 - Pf6N2E2_2118 0.11 +0.0 2,517,958 - Pf6N2E2_2118 0.11 +1.8 2,517,958 - Pf6N2E2_2118 0.11 -0.1 2,517,958 - Pf6N2E2_2118 0.11 -0.0 2,517,958 - Pf6N2E2_2118 0.11 +0.4 2,517,958 - Pf6N2E2_2118 0.11 +0.1 2,517,958 - Pf6N2E2_2118 0.11 -1.0 2,517,958 - Pf6N2E2_2118 0.11 +0.3 2,517,959 + Pf6N2E2_2118 0.11 +1.0 2,517,959 + Pf6N2E2_2118 0.11 +0.4 2,517,959 + Pf6N2E2_2118 0.11 -0.8 2,517,960 - Pf6N2E2_2118 0.11 +2.1 2,517,960 - Pf6N2E2_2118 0.11 +0.4 2,518,049 - Pf6N2E2_2118 0.14 +0.5 2,518,321 + Pf6N2E2_2118 0.22 +0.8 2,518,391 + Pf6N2E2_2118 0.24 -0.6 2,518,391 + Pf6N2E2_2118 0.24 -0.3 2,518,392 - Pf6N2E2_2118 0.24 -0.5 2,518,493 + Pf6N2E2_2118 0.27 -1.9 2,518,494 - Pf6N2E2_2118 0.27 +1.7
Or see this region's nucleotide sequence