Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1875 and Pf6N2E2_1876 are separated by 44 nucleotides Pf6N2E2_1876 and Pf6N2E2_1877 are separated by 23 nucleotides
Pf6N2E2_1875 - Protoporphyrinogen oxidase (EC 1.3.3.4), at 2,223,041 to 2,224,369
_1875
Pf6N2E2_1876 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 2,224,414 to 2,225,805
_1876
Pf6N2E2_1877 - putative transmembrane efflux protein., at 2,225,829 to 2,227,007
_1877
Position (kb)
2224
2225
2226 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2223.573 kb on - strand, within Pf6N2E2_1875 at 2223.759 kb on + strand, within Pf6N2E2_1875 at 2224.249 kb on + strand at 2224.315 kb on - strand at 2224.396 kb on - strand at 2224.454 kb on - strand at 2224.542 kb on + strand at 2224.543 kb on - strand at 2224.543 kb on - strand at 2224.672 kb on + strand, within Pf6N2E2_1876 at 2224.673 kb on - strand, within Pf6N2E2_1876 at 2225.113 kb on - strand, within Pf6N2E2_1876 at 2225.230 kb on - strand, within Pf6N2E2_1876 at 2225.372 kb on - strand, within Pf6N2E2_1876 at 2225.542 kb on - strand, within Pf6N2E2_1876 at 2225.868 kb on - strand at 2225.870 kb on - strand at 2225.953 kb on - strand, within Pf6N2E2_1877 at 2226.071 kb on - strand, within Pf6N2E2_1877 at 2226.534 kb on + strand, within Pf6N2E2_1877 at 2226.654 kb on + strand, within Pf6N2E2_1877 at 2226.654 kb on + strand, within Pf6N2E2_1877 at 2226.654 kb on + strand, within Pf6N2E2_1877 at 2226.654 kb on + strand, within Pf6N2E2_1877 at 2226.655 kb on - strand, within Pf6N2E2_1877
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 2,223,573 - Pf6N2E2_1875 0.40 -0.5 2,223,759 + Pf6N2E2_1875 0.54 -0.2 2,224,249 + -1.2 2,224,315 - +4.0 2,224,396 - -0.6 2,224,454 - +1.1 2,224,542 + -0.6 2,224,543 - +0.4 2,224,543 - -0.9 2,224,672 + Pf6N2E2_1876 0.19 +1.7 2,224,673 - Pf6N2E2_1876 0.19 -0.2 2,225,113 - Pf6N2E2_1876 0.50 +1.8 2,225,230 - Pf6N2E2_1876 0.59 +0.0 2,225,372 - Pf6N2E2_1876 0.69 +0.8 2,225,542 - Pf6N2E2_1876 0.81 -1.4 2,225,868 - -0.2 2,225,870 - +1.0 2,225,953 - Pf6N2E2_1877 0.11 +2.0 2,226,071 - Pf6N2E2_1877 0.21 +1.4 2,226,534 + Pf6N2E2_1877 0.60 +0.5 2,226,654 + Pf6N2E2_1877 0.70 +0.9 2,226,654 + Pf6N2E2_1877 0.70 -0.8 2,226,654 + Pf6N2E2_1877 0.70 +0.5 2,226,654 + Pf6N2E2_1877 0.70 -1.1 2,226,655 - Pf6N2E2_1877 0.70 -1.2
Or see this region's nucleotide sequence