Experiment: molybdate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_158 and Pf6N2E2_159 are separated by 5 nucleotides Pf6N2E2_159 and Pf6N2E2_160 are separated by 248 nucleotides Pf6N2E2_160 and Pf6N2E2_161 are separated by 133 nucleotides
Pf6N2E2_158 - Endonuclease I precursor (EC 3.1.21.1), at 212,509 to 213,198
_158
Pf6N2E2_159 - DNA damage resistance protein (from data) , at 213,204 to 213,452
_159
Pf6N2E2_160 - hypothetical protein, at 213,701 to 214,147
_160
Pf6N2E2_161 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 214,281 to 215,237
_161
Position (kb)
213
214
215 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 212.882 kb on + strand, within Pf6N2E2_158 at 212.882 kb on + strand, within Pf6N2E2_158 at 212.883 kb on - strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.968 kb on - strand, within Pf6N2E2_158 at 212.968 kb on - strand, within Pf6N2E2_158 at 212.968 kb on - strand, within Pf6N2E2_158 at 212.968 kb on - strand, within Pf6N2E2_158 at 212.968 kb on - strand, within Pf6N2E2_158 at 213.013 kb on + strand, within Pf6N2E2_158 at 213.014 kb on - strand, within Pf6N2E2_158 at 213.014 kb on - strand, within Pf6N2E2_158 at 213.017 kb on + strand, within Pf6N2E2_158 at 213.018 kb on - strand, within Pf6N2E2_158 at 213.018 kb on - strand, within Pf6N2E2_158 at 213.053 kb on + strand, within Pf6N2E2_158 at 213.053 kb on + strand, within Pf6N2E2_158 at 213.053 kb on + strand, within Pf6N2E2_158 at 213.058 kb on - strand, within Pf6N2E2_158 at 213.058 kb on - strand, within Pf6N2E2_158 at 213.068 kb on + strand, within Pf6N2E2_158 at 213.069 kb on - strand, within Pf6N2E2_158 at 213.069 kb on - strand, within Pf6N2E2_158 at 213.069 kb on - strand, within Pf6N2E2_158 at 213.069 kb on - strand, within Pf6N2E2_158 at 213.092 kb on + strand, within Pf6N2E2_158 at 213.092 kb on + strand, within Pf6N2E2_158 at 213.411 kb on - strand, within Pf6N2E2_159 at 213.411 kb on - strand, within Pf6N2E2_159 at 213.412 kb on + strand, within Pf6N2E2_159 at 213.412 kb on + strand, within Pf6N2E2_159 at 213.412 kb on + strand, within Pf6N2E2_159 at 213.413 kb on - strand, within Pf6N2E2_159 at 213.413 kb on - strand, within Pf6N2E2_159 at 213.420 kb on + strand, within Pf6N2E2_159 at 213.421 kb on - strand, within Pf6N2E2_159 at 213.428 kb on + strand at 213.580 kb on + strand at 213.592 kb on + strand at 213.592 kb on + strand at 213.593 kb on - strand at 213.995 kb on + strand, within Pf6N2E2_160 at 213.996 kb on - strand, within Pf6N2E2_160 at 214.069 kb on - strand, within Pf6N2E2_160 at 214.135 kb on + strand at 214.135 kb on + strand at 214.135 kb on + strand at 214.135 kb on + strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.197 kb on + strand at 214.198 kb on - strand at 214.202 kb on + strand at 214.202 kb on + strand at 214.202 kb on + strand at 214.203 kb on - strand at 214.203 kb on - strand at 214.257 kb on + strand at 214.257 kb on + strand at 214.257 kb on + strand at 214.258 kb on - strand at 214.258 kb on - strand at 214.264 kb on - strand at 214.264 kb on - strand at 214.455 kb on + strand, within Pf6N2E2_161 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.456 kb on - strand, within Pf6N2E2_161 at 214.753 kb on - strand, within Pf6N2E2_161 at 214.753 kb on - strand, within Pf6N2E2_161
Per-strain Table
Position Strand Gene LocusTag Fraction molybdate 0.25 M remove 212,882 + Pf6N2E2_158 0.54 -1.8 212,882 + Pf6N2E2_158 0.54 -0.8 212,883 - Pf6N2E2_158 0.54 -0.5 212,967 + Pf6N2E2_158 0.66 +0.8 212,967 + Pf6N2E2_158 0.66 -1.9 212,967 + Pf6N2E2_158 0.66 -0.9 212,967 + Pf6N2E2_158 0.66 -0.7 212,967 + Pf6N2E2_158 0.66 -0.5 212,967 + Pf6N2E2_158 0.66 -0.0 212,968 - Pf6N2E2_158 0.67 -1.0 212,968 - Pf6N2E2_158 0.67 +0.1 212,968 - Pf6N2E2_158 0.67 -1.7 212,968 - Pf6N2E2_158 0.67 -0.0 212,968 - Pf6N2E2_158 0.67 -0.2 213,013 + Pf6N2E2_158 0.73 -0.0 213,014 - Pf6N2E2_158 0.73 +1.3 213,014 - Pf6N2E2_158 0.73 -1.9 213,017 + Pf6N2E2_158 0.74 +0.8 213,018 - Pf6N2E2_158 0.74 -1.2 213,018 - Pf6N2E2_158 0.74 +0.6 213,053 + Pf6N2E2_158 0.79 +0.5 213,053 + Pf6N2E2_158 0.79 -0.0 213,053 + Pf6N2E2_158 0.79 -1.5 213,058 - Pf6N2E2_158 0.80 +0.3 213,058 - Pf6N2E2_158 0.80 +0.5 213,068 + Pf6N2E2_158 0.81 -0.0 213,069 - Pf6N2E2_158 0.81 +0.5 213,069 - Pf6N2E2_158 0.81 -0.7 213,069 - Pf6N2E2_158 0.81 -0.8 213,069 - Pf6N2E2_158 0.81 +0.1 213,092 + Pf6N2E2_158 0.84 +0.5 213,092 + Pf6N2E2_158 0.84 +0.3 213,411 - Pf6N2E2_159 0.83 -0.0 213,411 - Pf6N2E2_159 0.83 -0.5 213,412 + Pf6N2E2_159 0.84 +0.5 213,412 + Pf6N2E2_159 0.84 +0.4 213,412 + Pf6N2E2_159 0.84 -1.3 213,413 - Pf6N2E2_159 0.84 -0.5 213,413 - Pf6N2E2_159 0.84 -1.1 213,420 + Pf6N2E2_159 0.87 -1.0 213,421 - Pf6N2E2_159 0.87 +1.5 213,428 + -0.2 213,580 + +0.3 213,592 + +2.0 213,592 + +0.3 213,593 - +0.5 213,995 + Pf6N2E2_160 0.66 -1.6 213,996 - Pf6N2E2_160 0.66 -0.2 214,069 - Pf6N2E2_160 0.82 -1.6 214,135 + +1.0 214,135 + +0.3 214,135 + -1.2 214,135 + -0.2 214,136 - +0.1 214,136 - +0.8 214,136 - +1.9 214,136 - +0.4 214,136 - -0.8 214,136 - -0.1 214,136 - -0.5 214,197 + -0.9 214,198 - -0.2 214,202 + -0.7 214,202 + +0.1 214,202 + +0.5 214,203 - +0.4 214,203 - +0.1 214,257 + -2.0 214,257 + -0.6 214,257 + +0.1 214,258 - +0.6 214,258 - -0.7 214,264 - -1.2 214,264 - +0.3 214,455 + Pf6N2E2_161 0.18 +1.8 214,455 + Pf6N2E2_161 0.18 -2.1 214,455 + Pf6N2E2_161 0.18 -0.2 214,456 - Pf6N2E2_161 0.18 -2.2 214,753 - Pf6N2E2_161 0.49 +1.1 214,753 - Pf6N2E2_161 0.49 -1.3
Or see this region's nucleotide sequence