Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07725 and MMP_RS07730 are separated by 23 nucleotides MMP_RS07730 and MMP_RS07735 are separated by 16 nucleotides MMP_RS07735 and MMP_RS07740 are separated by 21 nucleotides MMP_RS07740 and MMP_RS07745 are separated by 14 nucleotides
MMP_RS07725: MMP_RS07725 - 4Fe-4S dicluster domain-containing protein, at 1,462,970 to 1,463,473
_RS07725
MMP_RS07730: MMP_RS07730 - pyruvate synthase subunit beta, at 1,463,497 to 1,464,393
_RS07730
MMP_RS07735: MMP_RS07735 - 2-ketoisovalerate ferredoxin oxidoreductase subunit alpha, at 1,464,410 to 1,465,573
_RS07735
MMP_RS07740: MMP_RS07740 - 4Fe-4S binding protein, at 1,465,595 to 1,465,852
_RS07740
MMP_RS07745: MMP_RS07745 - pyruvate ferredoxin oxidoreductase subunit gamma, at 1,465,867 to 1,466,397
_RS07745
Position (kb)
1464
1465
1466 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1463.740 kb on + strand, within MMP_RS07730 at 1463.848 kb on - strand, within MMP_RS07730 at 1463.892 kb on - strand, within MMP_RS07730 at 1463.893 kb on + strand, within MMP_RS07730 at 1464.006 kb on - strand, within MMP_RS07730 at 1464.393 kb on - strand at 1464.518 kb on - strand at 1464.773 kb on - strand, within MMP_RS07735 at 1464.993 kb on - strand, within MMP_RS07735 at 1465.827 kb on - strand at 1466.148 kb on - strand at 1466.189 kb on + strand at 1466.474 kb on + strand at 1466.534 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 1,463,740 + MMP_RS07730 0.27 -2.4 1,463,848 - MMP_RS07730 0.39 -0.6 1,463,892 - MMP_RS07730 0.44 -1.5 1,463,893 + MMP_RS07730 0.44 +0.5 1,464,006 - MMP_RS07730 0.57 -2.2 1,464,393 - -2.6 1,464,518 - -0.7 1,464,773 - MMP_RS07735 0.31 -1.3 1,464,993 - MMP_RS07735 0.50 -1.4 1,465,827 - -1.1 1,466,148 - -1.2 1,466,189 + +0.5 1,466,474 + -3.0 1,466,534 + +0.3
Or see this region's nucleotide sequence