Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06295 and MMP_RS06300 are separated by 289 nucleotides MMP_RS06300 and MMP_RS06305 are separated by 115 nucleotides MMP_RS06305 and MMP_RS06310 are separated by 19 nucleotides
MMP_RS06295: MMP_RS06295 - methanogenesis marker 14 protein, at 1,211,914 to 1,213,392
_RS06295
MMP_RS06300: MMP_RS06300 - ABC transporter substrate-binding protein, at 1,213,682 to 1,214,446
_RS06300
MMP_RS06305: MMP_RS06305 - transporter substrate-binding domain-containing protein, at 1,214,562 to 1,215,326
_RS06305
MMP_RS06310: MMP_RS06310 - NAD(P)/FAD-dependent oxidoreductase, at 1,215,346 to 1,216,503
_RS06310
Position (kb)
1213
1214
1215 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1212.791 kb on + strand, within MMP_RS06295 at 1212.947 kb on + strand, within MMP_RS06295 at 1213.158 kb on - strand, within MMP_RS06295 at 1213.174 kb on - strand, within MMP_RS06295 at 1213.174 kb on - strand, within MMP_RS06295 at 1213.394 kb on - strand at 1213.408 kb on + strand at 1213.413 kb on - strand at 1213.476 kb on - strand at 1213.476 kb on - strand at 1213.477 kb on + strand at 1213.614 kb on + strand at 1213.618 kb on + strand at 1213.628 kb on + strand at 1213.629 kb on - strand at 1213.630 kb on + strand at 1213.640 kb on - strand at 1213.647 kb on - strand at 1213.648 kb on + strand at 1213.651 kb on + strand at 1213.651 kb on + strand at 1213.654 kb on + strand at 1213.708 kb on + strand at 1213.747 kb on - strand at 1213.748 kb on + strand at 1213.779 kb on - strand, within MMP_RS06300 at 1213.833 kb on + strand, within MMP_RS06300 at 1214.030 kb on - strand, within MMP_RS06300 at 1214.030 kb on - strand, within MMP_RS06300 at 1214.130 kb on + strand, within MMP_RS06300 at 1214.130 kb on + strand, within MMP_RS06300 at 1214.187 kb on + strand, within MMP_RS06300 at 1214.217 kb on - strand, within MMP_RS06300 at 1214.218 kb on + strand, within MMP_RS06300 at 1214.220 kb on + strand, within MMP_RS06300 at 1214.230 kb on - strand, within MMP_RS06300 at 1214.242 kb on + strand, within MMP_RS06300 at 1214.539 kb on + strand at 1214.539 kb on + strand at 1214.546 kb on + strand at 1214.678 kb on + strand, within MMP_RS06305 at 1214.703 kb on - strand, within MMP_RS06305 at 1214.704 kb on + strand, within MMP_RS06305 at 1214.847 kb on + strand, within MMP_RS06305 at 1215.060 kb on - strand, within MMP_RS06305 at 1215.159 kb on + strand, within MMP_RS06305 at 1215.175 kb on + strand, within MMP_RS06305 at 1215.218 kb on - strand, within MMP_RS06305 at 1215.258 kb on + strand at 1215.259 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 1,212,791 + MMP_RS06295 0.59 -1.9 1,212,947 + MMP_RS06295 0.70 -2.5 1,213,158 - MMP_RS06295 0.84 -2.0 1,213,174 - MMP_RS06295 0.85 -2.4 1,213,174 - MMP_RS06295 0.85 -3.4 1,213,394 - -1.8 1,213,408 + -0.4 1,213,413 - -0.1 1,213,476 - -0.1 1,213,476 - -0.1 1,213,477 + +0.3 1,213,614 + +0.2 1,213,618 + -0.0 1,213,628 + -0.2 1,213,629 - -0.2 1,213,630 + +0.2 1,213,640 - -0.5 1,213,647 - +0.5 1,213,648 + +0.3 1,213,651 + -0.8 1,213,651 + +0.2 1,213,654 + -0.2 1,213,708 + +0.2 1,213,747 - +0.2 1,213,748 + -0.4 1,213,779 - MMP_RS06300 0.13 +0.6 1,213,833 + MMP_RS06300 0.20 +0.4 1,214,030 - MMP_RS06300 0.45 +0.1 1,214,030 - MMP_RS06300 0.45 -0.1 1,214,130 + MMP_RS06300 0.59 +0.0 1,214,130 + MMP_RS06300 0.59 +0.0 1,214,187 + MMP_RS06300 0.66 +0.6 1,214,217 - MMP_RS06300 0.70 +0.1 1,214,218 + MMP_RS06300 0.70 +0.2 1,214,220 + MMP_RS06300 0.70 -0.1 1,214,230 - MMP_RS06300 0.72 +0.3 1,214,242 + MMP_RS06300 0.73 +0.2 1,214,539 + -0.9 1,214,539 + -0.0 1,214,546 + -0.0 1,214,678 + MMP_RS06305 0.15 +0.2 1,214,703 - MMP_RS06305 0.18 +0.4 1,214,704 + MMP_RS06305 0.19 -0.1 1,214,847 + MMP_RS06305 0.37 +0.2 1,215,060 - MMP_RS06305 0.65 +0.1 1,215,159 + MMP_RS06305 0.78 -0.4 1,215,175 + MMP_RS06305 0.80 -0.2 1,215,218 - MMP_RS06305 0.86 +0.1 1,215,258 + +0.3 1,215,259 - -0.1
Or see this region's nucleotide sequence