Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05950 and MMP_RS05955 are separated by 227 nucleotides MMP_RS05955 and MMP_RS05960 are separated by 12 nucleotides MMP_RS05960 and MMP_RS05965 are separated by 30 nucleotides MMP_RS05965 and MMP_RS05970 are separated by 13 nucleotides MMP_RS05970 and MMP_RS05975 are separated by 20 nucleotides
MMP_RS05950: MMP_RS05950 - nickel-dependent hydrogenase large subunit, at 1,141,444 to 1,142,571
_RS05950
MMP_RS05955: MMP_RS05955 - CoB--CoM heterodisulfide reductase subunit C, at 1,142,799 to 1,143,377
_RS05955
MMP_RS05960: MMP_RS05960 - CoB--CoM heterodisulfide reductase subunit B, at 1,143,390 to 1,144,295
_RS05960
MMP_RS05965: MMP_RS05965 - carboxymuconolactone decarboxylase family protein, at 1,144,326 to 1,144,640
_RS05965
MMP_RS05970: MMP_RS05970 - class II SORL domain-containing protein, at 1,144,654 to 1,145,010
_RS05970
MMP_RS05975: MMP_RS05975 - hypothetical protein, at 1,145,031 to 1,145,297
_RS05975
Position (kb)
1143
1144
1145 Strain fitness (log2 ratio)
-1
0
1 at 1142.401 kb on + strand, within MMP_RS05950 at 1142.466 kb on - strand at 1142.504 kb on + strand at 1142.533 kb on + strand at 1142.568 kb on + strand at 1142.619 kb on + strand at 1142.636 kb on - strand at 1142.650 kb on + strand at 1142.659 kb on + strand at 1142.665 kb on - strand at 1142.675 kb on - strand at 1142.679 kb on - strand at 1142.679 kb on - strand at 1142.679 kb on - strand at 1142.680 kb on + strand at 1142.688 kb on + strand at 1142.723 kb on + strand at 1142.725 kb on - strand at 1142.727 kb on - strand at 1142.729 kb on - strand at 1142.729 kb on - strand at 1142.730 kb on + strand at 1142.730 kb on + strand at 1142.746 kb on - strand at 1142.746 kb on - strand at 1142.747 kb on + strand at 1142.747 kb on + strand at 1142.747 kb on + strand at 1142.747 kb on + strand at 1142.748 kb on - strand at 1142.748 kb on - strand at 1142.749 kb on + strand at 1142.750 kb on - strand at 1142.751 kb on + strand at 1142.777 kb on - strand at 1143.032 kb on - strand, within MMP_RS05955 at 1143.098 kb on + strand, within MMP_RS05955 at 1143.486 kb on + strand, within MMP_RS05960 at 1143.675 kb on + strand, within MMP_RS05960 at 1143.709 kb on - strand, within MMP_RS05960 at 1143.742 kb on + strand, within MMP_RS05960 at 1143.744 kb on + strand, within MMP_RS05960 at 1143.769 kb on - strand, within MMP_RS05960 at 1143.778 kb on - strand, within MMP_RS05960 at 1143.779 kb on + strand, within MMP_RS05960 at 1143.783 kb on - strand, within MMP_RS05960 at 1143.856 kb on + strand, within MMP_RS05960 at 1143.871 kb on - strand, within MMP_RS05960 at 1143.871 kb on - strand, within MMP_RS05960 at 1143.886 kb on - strand, within MMP_RS05960 at 1143.886 kb on - strand, within MMP_RS05960 at 1143.887 kb on + strand, within MMP_RS05960 at 1143.887 kb on + strand, within MMP_RS05960 at 1143.958 kb on - strand, within MMP_RS05960 at 1143.960 kb on - strand, within MMP_RS05960 at 1143.960 kb on - strand, within MMP_RS05960 at 1143.961 kb on + strand, within MMP_RS05960 at 1143.961 kb on + strand, within MMP_RS05960 at 1143.963 kb on - strand, within MMP_RS05960 at 1143.964 kb on + strand, within MMP_RS05960 at 1143.977 kb on - strand, within MMP_RS05960 at 1144.193 kb on + strand, within MMP_RS05960 at 1144.293 kb on - strand at 1144.544 kb on + strand, within MMP_RS05965 at 1144.580 kb on + strand, within MMP_RS05965 at 1144.580 kb on + strand, within MMP_RS05965 at 1144.653 kb on + strand at 1144.785 kb on + strand, within MMP_RS05970 at 1144.829 kb on - strand, within MMP_RS05970 at 1144.830 kb on + strand, within MMP_RS05970 at 1144.853 kb on - strand, within MMP_RS05970 at 1145.108 kb on - strand, within MMP_RS05975 at 1145.288 kb on - strand at 1145.292 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 1,142,401 + MMP_RS05950 0.85 -0.1 1,142,466 - +0.3 1,142,504 + -0.5 1,142,533 + -0.3 1,142,568 + -0.5 1,142,619 + -0.1 1,142,636 - -1.0 1,142,650 + +0.0 1,142,659 + -0.8 1,142,665 - -0.1 1,142,675 - -0.6 1,142,679 - -0.2 1,142,679 - -0.1 1,142,679 - +0.7 1,142,680 + +0.1 1,142,688 + +0.1 1,142,723 + -0.1 1,142,725 - +0.1 1,142,727 - -0.2 1,142,729 - -0.3 1,142,729 - -0.1 1,142,730 + -0.1 1,142,730 + -0.0 1,142,746 - +0.1 1,142,746 - +0.0 1,142,747 + +0.2 1,142,747 + +0.5 1,142,747 + +0.1 1,142,747 + +0.1 1,142,748 - +0.8 1,142,748 - -0.2 1,142,749 + -0.0 1,142,750 - -0.0 1,142,751 + +0.4 1,142,777 - -0.1 1,143,032 - MMP_RS05955 0.40 -0.4 1,143,098 + MMP_RS05955 0.52 +0.1 1,143,486 + MMP_RS05960 0.11 -0.1 1,143,675 + MMP_RS05960 0.31 -0.2 1,143,709 - MMP_RS05960 0.35 -0.2 1,143,742 + MMP_RS05960 0.39 +0.5 1,143,744 + MMP_RS05960 0.39 +0.4 1,143,769 - MMP_RS05960 0.42 +0.1 1,143,778 - MMP_RS05960 0.43 +0.1 1,143,779 + MMP_RS05960 0.43 +0.3 1,143,783 - MMP_RS05960 0.43 +0.4 1,143,856 + MMP_RS05960 0.51 +0.3 1,143,871 - MMP_RS05960 0.53 +0.3 1,143,871 - MMP_RS05960 0.53 +0.9 1,143,886 - MMP_RS05960 0.55 +0.3 1,143,886 - MMP_RS05960 0.55 +0.0 1,143,887 + MMP_RS05960 0.55 -0.3 1,143,887 + MMP_RS05960 0.55 -0.2 1,143,958 - MMP_RS05960 0.63 +0.9 1,143,960 - MMP_RS05960 0.63 +0.2 1,143,960 - MMP_RS05960 0.63 +0.2 1,143,961 + MMP_RS05960 0.63 -0.0 1,143,961 + MMP_RS05960 0.63 -0.3 1,143,963 - MMP_RS05960 0.63 +0.1 1,143,964 + MMP_RS05960 0.63 +0.5 1,143,977 - MMP_RS05960 0.65 +0.4 1,144,193 + MMP_RS05960 0.89 +0.0 1,144,293 - +0.0 1,144,544 + MMP_RS05965 0.69 -0.2 1,144,580 + MMP_RS05965 0.81 -0.2 1,144,580 + MMP_RS05965 0.81 -0.2 1,144,653 + +0.1 1,144,785 + MMP_RS05970 0.37 -0.0 1,144,829 - MMP_RS05970 0.49 +0.2 1,144,830 + MMP_RS05970 0.49 -0.0 1,144,853 - MMP_RS05970 0.56 -0.5 1,145,108 - MMP_RS05975 0.29 +0.2 1,145,288 - -0.2 1,145,292 + -0.1
Or see this region's nucleotide sequence