Experiment: Formate McCas no stress control 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02340 and MMP_RS02345 are separated by 31 nucleotides MMP_RS02345 and MMP_RS02350 are separated by 6 nucleotides MMP_RS02350 and MMP_RS02355 are separated by 1 nucleotides MMP_RS02355 and MMP_RS02360 are separated by 97 nucleotides MMP_RS02360 and MMP_RS02365 are separated by 206 nucleotides
MMP_RS02340: MMP_RS02340 - DNA-directed RNA polymerase subunit E'', at 440,245 to 440,421
_RS02340
MMP_RS02345: MMP_RS02345 - GTP-dependent dephospho-CoA kinase family protein, at 440,453 to 440,929
_RS02345
MMP_RS02350: MMP_RS02350 - 30S ribosomal protein S24e, at 440,936 to 441,244
_RS02350
MMP_RS02355: MMP_RS02355 - 30S ribosomal protein S27ae, at 441,246 to 441,449
_RS02355
MMP_RS02360: MMP_RS02360 - hypothetical protein, at 441,547 to 441,783
_RS02360
MMP_RS02365: MMP_RS02365 - nitrogen fixation, at 441,990 to 442,316
_RS02365
Position (kb)
441
442 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 440.337 kb on + strand, within MMP_RS02340 at 440.390 kb on - strand, within MMP_RS02340 at 440.391 kb on + strand, within MMP_RS02340 at 440.401 kb on - strand, within MMP_RS02340 at 440.415 kb on - strand at 440.420 kb on + strand at 440.439 kb on - strand at 440.441 kb on - strand at 440.441 kb on - strand at 440.457 kb on + strand at 440.460 kb on - strand at 440.475 kb on - strand at 440.530 kb on + strand, within MMP_RS02345 at 440.587 kb on - strand, within MMP_RS02345 at 440.587 kb on - strand, within MMP_RS02345 at 440.587 kb on - strand, within MMP_RS02345 at 440.601 kb on - strand, within MMP_RS02345 at 440.740 kb on + strand, within MMP_RS02345 at 440.808 kb on + strand, within MMP_RS02345 at 440.951 kb on + strand at 440.966 kb on + strand at 441.254 kb on - strand at 441.292 kb on + strand, within MMP_RS02355 at 441.316 kb on - strand, within MMP_RS02355 at 441.359 kb on + strand, within MMP_RS02355 at 441.465 kb on + strand at 441.507 kb on + strand at 441.527 kb on - strand at 441.538 kb on - strand at 441.538 kb on - strand at 441.544 kb on - strand at 441.545 kb on + strand at 441.548 kb on - strand at 441.602 kb on - strand, within MMP_RS02360 at 441.602 kb on - strand, within MMP_RS02360 at 441.602 kb on - strand, within MMP_RS02360 at 441.603 kb on + strand, within MMP_RS02360 at 441.604 kb on - strand, within MMP_RS02360 at 441.604 kb on - strand, within MMP_RS02360 at 441.605 kb on + strand, within MMP_RS02360 at 441.799 kb on + strand at 441.801 kb on + strand at 441.812 kb on - strand at 441.827 kb on + strand at 441.840 kb on - strand at 441.841 kb on + strand at 441.847 kb on - strand at 441.859 kb on - strand at 441.859 kb on - strand at 441.878 kb on - strand at 441.879 kb on + strand at 441.996 kb on + strand at 442.017 kb on + strand at 442.020 kb on - strand at 442.046 kb on - strand, within MMP_RS02365 at 442.068 kb on - strand, within MMP_RS02365 at 442.068 kb on - strand, within MMP_RS02365 at 442.069 kb on + strand, within MMP_RS02365 at 442.120 kb on - strand, within MMP_RS02365 at 442.121 kb on + strand, within MMP_RS02365 at 442.121 kb on + strand, within MMP_RS02365 at 442.121 kb on + strand, within MMP_RS02365 at 442.122 kb on - strand, within MMP_RS02365 at 442.123 kb on + strand, within MMP_RS02365 at 442.123 kb on + strand, within MMP_RS02365 at 442.128 kb on - strand, within MMP_RS02365 at 442.129 kb on + strand, within MMP_RS02365 at 442.219 kb on + strand, within MMP_RS02365 at 442.219 kb on + strand, within MMP_RS02365 at 442.248 kb on - strand, within MMP_RS02365 at 442.297 kb on + strand at 442.361 kb on - strand at 442.361 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.422 kb on - strand at 442.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 1 remove 440,337 + MMP_RS02340 0.52 +0.6 440,390 - MMP_RS02340 0.82 -1.4 440,391 + MMP_RS02340 0.82 +0.7 440,401 - MMP_RS02340 0.88 +0.1 440,415 - -1.0 440,420 + -0.4 440,439 - -0.8 440,441 - -0.5 440,441 - -0.2 440,457 + -1.4 440,460 - -1.9 440,475 - -1.6 440,530 + MMP_RS02345 0.16 -1.3 440,587 - MMP_RS02345 0.28 -1.2 440,587 - MMP_RS02345 0.28 -0.4 440,587 - MMP_RS02345 0.28 -1.5 440,601 - MMP_RS02345 0.31 -0.6 440,740 + MMP_RS02345 0.60 -0.7 440,808 + MMP_RS02345 0.74 -3.3 440,951 + -2.1 440,966 + -0.5 441,254 - -1.8 441,292 + MMP_RS02355 0.23 -2.3 441,316 - MMP_RS02355 0.34 -1.4 441,359 + MMP_RS02355 0.55 -2.2 441,465 + +0.2 441,507 + +0.1 441,527 - +0.1 441,538 - -0.2 441,538 - -0.3 441,544 - -0.2 441,545 + +0.6 441,548 - +0.1 441,602 - MMP_RS02360 0.23 +0.6 441,602 - MMP_RS02360 0.23 -0.0 441,602 - MMP_RS02360 0.23 +0.2 441,603 + MMP_RS02360 0.24 +0.0 441,604 - MMP_RS02360 0.24 +0.0 441,604 - MMP_RS02360 0.24 +0.9 441,605 + MMP_RS02360 0.24 +0.4 441,799 + -0.4 441,801 + +1.2 441,812 - +0.2 441,827 + +0.2 441,840 - +0.4 441,841 + +0.0 441,847 - +1.1 441,859 - -0.2 441,859 - -0.0 441,878 - +0.1 441,879 + +0.6 441,996 + +0.1 442,017 + +0.4 442,020 - +0.4 442,046 - MMP_RS02365 0.17 -0.3 442,068 - MMP_RS02365 0.24 -0.1 442,068 - MMP_RS02365 0.24 +0.6 442,069 + MMP_RS02365 0.24 -0.2 442,120 - MMP_RS02365 0.40 +0.1 442,121 + MMP_RS02365 0.40 +0.4 442,121 + MMP_RS02365 0.40 -0.1 442,121 + MMP_RS02365 0.40 +0.0 442,122 - MMP_RS02365 0.40 +1.7 442,123 + MMP_RS02365 0.41 +0.3 442,123 + MMP_RS02365 0.41 +0.3 442,128 - MMP_RS02365 0.42 -0.3 442,129 + MMP_RS02365 0.43 +0.3 442,219 + MMP_RS02365 0.70 +0.7 442,219 + MMP_RS02365 0.70 -0.1 442,248 - MMP_RS02365 0.79 -0.1 442,297 + -0.3 442,361 - -0.6 442,361 - +0.2 442,418 - +0.1 442,418 - -0.4 442,418 - +1.1 442,418 - +0.3 442,418 - -0.2 442,418 - -0.1 442,418 - +0.5 442,418 - +0.1 442,418 - -0.1 442,418 - +0.3 442,418 - +0.0 442,418 - -0.1 442,418 - -0.0 442,418 - +0.2 442,419 + -0.4 442,419 + -0.1 442,419 + +0.5 442,419 + -0.2 442,419 + -0.1 442,419 + -0.1 442,422 - -0.8 442,423 + +0.5
Or see this region's nucleotide sequence