Experiment: Recovery broth with Warfarin 1.0 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20425 and BBR_RS20430 are separated by 45 nucleotides BBR_RS20430 and BBR_RS20435 are separated by 128 nucleotides BBR_RS20435 and BBR_RS20440 are separated by 86 nucleotides
BBR_RS20425: BBR_RS20425 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 2,405,097 to 2,406,044
_RS20425
BBR_RS20430: BBR_RS20430 - hypothetical protein, at 2,406,090 to 2,406,719
_RS20430
BBR_RS20435: BBR_RS20435 - CCA tRNA nucleotidyltransferase, at 2,406,848 to 2,408,266
_RS20435
BBR_RS20440: BBR_RS20440 - NUDIX hydrolase, at 2,408,353 to 2,409,324
_RS20440
Position (kb)
2406
2407
2408
2409 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2406.018 kb on - strand at 2406.018 kb on - strand at 2406.023 kb on + strand at 2406.039 kb on - strand at 2406.053 kb on - strand at 2406.053 kb on - strand at 2406.053 kb on - strand at 2406.055 kb on + strand at 2406.058 kb on - strand at 2406.069 kb on - strand at 2406.072 kb on + strand at 2406.082 kb on - strand at 2406.086 kb on + strand at 2406.086 kb on + strand at 2406.090 kb on - strand at 2406.090 kb on - strand at 2406.124 kb on + strand at 2406.125 kb on + strand at 2406.141 kb on + strand at 2406.164 kb on + strand, within BBR_RS20430 at 2406.177 kb on - strand, within BBR_RS20430 at 2406.195 kb on - strand, within BBR_RS20430 at 2406.221 kb on + strand, within BBR_RS20430 at 2406.255 kb on + strand, within BBR_RS20430 at 2406.288 kb on + strand, within BBR_RS20430 at 2406.310 kb on - strand, within BBR_RS20430 at 2406.333 kb on + strand, within BBR_RS20430 at 2406.386 kb on + strand, within BBR_RS20430 at 2406.394 kb on - strand, within BBR_RS20430 at 2406.494 kb on + strand, within BBR_RS20430 at 2406.502 kb on - strand, within BBR_RS20430 at 2406.502 kb on - strand, within BBR_RS20430 at 2406.578 kb on - strand, within BBR_RS20430 at 2406.582 kb on + strand, within BBR_RS20430 at 2406.600 kb on + strand, within BBR_RS20430 at 2406.650 kb on + strand, within BBR_RS20430 at 2406.658 kb on - strand at 2406.658 kb on - strand at 2406.700 kb on + strand at 2406.701 kb on + strand at 2406.701 kb on + strand at 2406.703 kb on - strand at 2406.703 kb on - strand at 2406.708 kb on - strand at 2406.708 kb on - strand at 2406.708 kb on - strand at 2406.709 kb on - strand at 2406.711 kb on + strand at 2406.714 kb on + strand at 2406.726 kb on - strand at 2406.739 kb on + strand at 2406.739 kb on + strand at 2406.739 kb on + strand at 2406.747 kb on - strand at 2406.747 kb on - strand at 2406.747 kb on - strand at 2406.747 kb on - strand at 2406.749 kb on + strand at 2406.749 kb on + strand at 2406.757 kb on - strand at 2406.757 kb on - strand at 2406.757 kb on - strand at 2406.765 kb on + strand at 2406.767 kb on - strand at 2406.777 kb on - strand at 2406.793 kb on + strand at 2406.793 kb on + strand at 2406.793 kb on + strand at 2406.793 kb on + strand at 2406.793 kb on + strand at 2406.797 kb on + strand at 2406.799 kb on + strand at 2406.799 kb on + strand at 2406.799 kb on + strand at 2406.799 kb on + strand at 2406.799 kb on + strand at 2406.799 kb on + strand at 2406.799 kb on + strand at 2406.799 kb on + strand at 2406.801 kb on - strand at 2406.801 kb on - strand at 2406.801 kb on - strand at 2406.807 kb on - strand at 2406.807 kb on - strand at 2406.807 kb on - strand at 2406.807 kb on - strand at 2406.807 kb on - strand at 2406.807 kb on - strand at 2406.807 kb on - strand at 2406.807 kb on - strand at 2406.807 kb on - strand at 2406.812 kb on + strand at 2406.815 kb on - strand at 2406.815 kb on - strand at 2406.815 kb on - strand at 2406.815 kb on - strand at 2406.815 kb on - strand at 2406.817 kb on + strand at 2406.817 kb on + strand at 2406.820 kb on + strand at 2406.825 kb on - strand at 2406.825 kb on - strand at 2406.838 kb on + strand at 2406.841 kb on - strand at 2406.846 kb on - strand at 2406.846 kb on - strand at 2406.846 kb on - strand at 2406.846 kb on - strand at 2406.864 kb on + strand at 2407.189 kb on + strand, within BBR_RS20435 at 2408.281 kb on - strand at 2408.306 kb on + strand at 2408.311 kb on + strand at 2408.311 kb on + strand at 2408.311 kb on + strand at 2408.311 kb on + strand at 2408.311 kb on + strand at 2408.311 kb on + strand at 2408.311 kb on + strand at 2408.311 kb on + strand at 2408.319 kb on - strand at 2408.319 kb on - strand at 2408.319 kb on - strand at 2408.319 kb on - strand at 2408.319 kb on - strand at 2408.319 kb on - strand at 2408.319 kb on - strand at 2408.319 kb on - strand at 2408.319 kb on - strand at 2408.319 kb on - strand at 2408.320 kb on + strand at 2408.320 kb on + strand at 2408.328 kb on - strand at 2408.332 kb on + strand at 2408.332 kb on + strand at 2408.332 kb on + strand at 2408.332 kb on + strand at 2408.332 kb on + strand at 2408.332 kb on + strand at 2408.332 kb on + strand at 2408.332 kb on + strand at 2408.332 kb on + strand at 2408.337 kb on + strand at 2408.340 kb on - strand at 2408.340 kb on - strand at 2408.340 kb on - strand at 2408.340 kb on - strand at 2408.340 kb on - strand at 2408.340 kb on - strand at 2408.340 kb on - strand at 2408.340 kb on - strand at 2408.340 kb on - strand at 2408.345 kb on - strand at 2408.364 kb on + strand at 2408.364 kb on + strand at 2408.430 kb on + strand at 2408.430 kb on + strand at 2408.430 kb on + strand at 2408.430 kb on + strand at 2408.430 kb on + strand at 2408.430 kb on + strand at 2408.430 kb on + strand at 2408.430 kb on + strand at 2408.430 kb on + strand at 2408.433 kb on + strand at 2408.475 kb on + strand, within BBR_RS20440 at 2408.485 kb on + strand, within BBR_RS20440 at 2408.485 kb on + strand, within BBR_RS20440 at 2408.485 kb on + strand, within BBR_RS20440 at 2408.485 kb on + strand, within BBR_RS20440 at 2408.493 kb on + strand, within BBR_RS20440 at 2408.521 kb on + strand, within BBR_RS20440 at 2408.533 kb on + strand, within BBR_RS20440 at 2408.533 kb on + strand, within BBR_RS20440 at 2408.635 kb on + strand, within BBR_RS20440 at 2408.691 kb on + strand, within BBR_RS20440 at 2408.736 kb on + strand, within BBR_RS20440 at 2408.736 kb on + strand, within BBR_RS20440 at 2408.737 kb on + strand, within BBR_RS20440 at 2408.786 kb on + strand, within BBR_RS20440 at 2408.801 kb on + strand, within BBR_RS20440 at 2408.808 kb on + strand, within BBR_RS20440 at 2408.957 kb on + strand, within BBR_RS20440 at 2408.977 kb on + strand, within BBR_RS20440 at 2408.991 kb on + strand, within BBR_RS20440 at 2408.991 kb on + strand, within BBR_RS20440 at 2409.039 kb on + strand, within BBR_RS20440 at 2409.046 kb on + strand, within BBR_RS20440 at 2409.074 kb on + strand, within BBR_RS20440 at 2409.132 kb on + strand, within BBR_RS20440 at 2409.142 kb on + strand, within BBR_RS20440 at 2409.154 kb on + strand, within BBR_RS20440 at 2409.246 kb on - strand at 2409.260 kb on + strand at 2409.260 kb on + strand at 2409.260 kb on + strand at 2409.260 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Warfarin 1.0 mM remove 2,406,018 - +0.2 2,406,018 - -0.2 2,406,023 + -0.7 2,406,039 - -1.3 2,406,053 - -0.6 2,406,053 - +0.3 2,406,053 - +0.8 2,406,055 + -0.6 2,406,058 - +0.7 2,406,069 - +1.3 2,406,072 + -0.2 2,406,082 - +0.4 2,406,086 + +0.2 2,406,086 + +0.3 2,406,090 - +0.4 2,406,090 - +0.9 2,406,124 + +2.5 2,406,125 + +0.7 2,406,141 + +2.5 2,406,164 + BBR_RS20430 0.12 +1.5 2,406,177 - BBR_RS20430 0.14 +2.5 2,406,195 - BBR_RS20430 0.17 +2.5 2,406,221 + BBR_RS20430 0.21 +2.5 2,406,255 + BBR_RS20430 0.26 +1.5 2,406,288 + BBR_RS20430 0.31 +2.5 2,406,310 - BBR_RS20430 0.35 +2.5 2,406,333 + BBR_RS20430 0.39 +1.5 2,406,386 + BBR_RS20430 0.47 +2.5 2,406,394 - BBR_RS20430 0.48 +1.5 2,406,494 + BBR_RS20430 0.64 +2.5 2,406,502 - BBR_RS20430 0.65 +2.5 2,406,502 - BBR_RS20430 0.65 +1.5 2,406,578 - BBR_RS20430 0.77 +2.5 2,406,582 + BBR_RS20430 0.78 +2.5 2,406,600 + BBR_RS20430 0.81 +0.9 2,406,650 + BBR_RS20430 0.89 +1.5 2,406,658 - +0.8 2,406,658 - +1.5 2,406,700 + -0.6 2,406,701 + +0.3 2,406,701 + +0.2 2,406,703 - +0.6 2,406,703 - +0.9 2,406,708 - -0.2 2,406,708 - -0.2 2,406,708 - -0.4 2,406,709 - +0.4 2,406,711 + -0.6 2,406,714 + -0.2 2,406,726 - -0.0 2,406,739 + +0.3 2,406,739 + -0.2 2,406,739 + -0.9 2,406,747 - -1.5 2,406,747 - +0.8 2,406,747 - +0.9 2,406,747 - -0.1 2,406,749 + +0.4 2,406,749 + +0.0 2,406,757 - -0.4 2,406,757 - +0.6 2,406,757 - +0.3 2,406,765 + -0.1 2,406,767 - -0.1 2,406,777 - +0.6 2,406,793 + -0.1 2,406,793 + +0.4 2,406,793 + +0.0 2,406,793 + +0.2 2,406,793 + +0.1 2,406,797 + +0.3 2,406,799 + +1.1 2,406,799 + -0.3 2,406,799 + -0.1 2,406,799 + -1.3 2,406,799 + +0.8 2,406,799 + -0.4 2,406,799 + -0.0 2,406,799 + -0.2 2,406,801 - +0.0 2,406,801 - -0.1 2,406,801 - -0.3 2,406,807 - -1.2 2,406,807 - +0.8 2,406,807 - +0.7 2,406,807 - +0.0 2,406,807 - +0.2 2,406,807 - -0.1 2,406,807 - -0.2 2,406,807 - -0.1 2,406,807 - +0.3 2,406,812 + -0.3 2,406,815 - +0.5 2,406,815 - -0.5 2,406,815 - -0.0 2,406,815 - +0.2 2,406,815 - +0.2 2,406,817 + +0.3 2,406,817 + +0.1 2,406,820 + +0.6 2,406,825 - +0.2 2,406,825 - -0.1 2,406,838 + +0.0 2,406,841 - +0.4 2,406,846 - +0.3 2,406,846 - -0.2 2,406,846 - -0.3 2,406,846 - +0.2 2,406,864 + +1.1 2,407,189 + BBR_RS20435 0.24 -2.9 2,408,281 - +0.6 2,408,306 + +0.8 2,408,311 + +0.1 2,408,311 + -0.5 2,408,311 + +0.3 2,408,311 + -0.1 2,408,311 + +0.1 2,408,311 + +0.5 2,408,311 + -0.2 2,408,311 + +0.3 2,408,319 - -0.2 2,408,319 - +0.5 2,408,319 - -0.1 2,408,319 - -0.7 2,408,319 - +0.2 2,408,319 - -0.7 2,408,319 - +0.2 2,408,319 - -0.4 2,408,319 - +0.0 2,408,319 - +0.3 2,408,320 + -0.2 2,408,320 + -2.0 2,408,328 - +0.9 2,408,332 + -0.2 2,408,332 + +0.8 2,408,332 + -0.4 2,408,332 + -0.4 2,408,332 + +0.4 2,408,332 + +0.1 2,408,332 + +0.1 2,408,332 + +0.3 2,408,332 + -0.3 2,408,337 + -1.9 2,408,340 - -2.9 2,408,340 - +0.1 2,408,340 - -0.1 2,408,340 - -1.1 2,408,340 - -1.8 2,408,340 - -0.5 2,408,340 - +0.1 2,408,340 - -0.1 2,408,340 - -0.7 2,408,345 - -0.4 2,408,364 + -0.9 2,408,364 + -0.2 2,408,430 + -1.1 2,408,430 + +0.9 2,408,430 + -0.8 2,408,430 + -0.3 2,408,430 + -0.2 2,408,430 + -0.2 2,408,430 + -0.4 2,408,430 + -1.4 2,408,430 + +0.5 2,408,433 + -0.7 2,408,475 + BBR_RS20440 0.13 -0.0 2,408,485 + BBR_RS20440 0.14 -0.8 2,408,485 + BBR_RS20440 0.14 -1.1 2,408,485 + BBR_RS20440 0.14 -0.9 2,408,485 + BBR_RS20440 0.14 -1.1 2,408,493 + BBR_RS20440 0.14 -1.6 2,408,521 + BBR_RS20440 0.17 +1.5 2,408,533 + BBR_RS20440 0.19 +1.2 2,408,533 + BBR_RS20440 0.19 +0.5 2,408,635 + BBR_RS20440 0.29 -1.1 2,408,691 + BBR_RS20440 0.35 +0.9 2,408,736 + BBR_RS20440 0.39 +0.1 2,408,736 + BBR_RS20440 0.39 -0.8 2,408,737 + BBR_RS20440 0.40 -0.3 2,408,786 + BBR_RS20440 0.45 +0.5 2,408,801 + BBR_RS20440 0.46 +0.2 2,408,808 + BBR_RS20440 0.47 +0.4 2,408,957 + BBR_RS20440 0.62 -0.3 2,408,977 + BBR_RS20440 0.64 +0.9 2,408,991 + BBR_RS20440 0.66 -0.6 2,408,991 + BBR_RS20440 0.66 -1.3 2,409,039 + BBR_RS20440 0.71 -0.9 2,409,046 + BBR_RS20440 0.71 +0.2 2,409,074 + BBR_RS20440 0.74 -1.3 2,409,132 + BBR_RS20440 0.80 -2.3 2,409,142 + BBR_RS20440 0.81 -0.5 2,409,154 + BBR_RS20440 0.82 +2.5 2,409,246 - -0.3 2,409,260 + +0.9 2,409,260 + -2.6 2,409,260 + +0.9 2,409,260 + -1.6
Or see this region's nucleotide sequence