Experiment: Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20935 and IAI47_20940 are separated by 149 nucleotides IAI47_20940 and IAI47_20945 are separated by 71 nucleotides IAI47_20945 and IAI47_20950 are separated by 394 nucleotides
IAI47_20935: IAI47_20935 - ABC transporter ATP-binding protein, at 434,918 to 436,036
_20935
IAI47_20940: IAI47_20940 - hypothetical protein, at 436,186 to 436,743
_20940
IAI47_20945: IAI47_20945 - hypothetical protein, at 436,815 to 437,156
_20945
IAI47_20950: IAI47_20950 - DUF2628 domain-containing protein, at 437,551 to 437,988
_20950
Position (kb) 436
437 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 435.203 kb on - strand, within IAI47_20935 at 435.223 kb on - strand, within IAI47_20935 at 435.305 kb on - strand, within IAI47_20935 at 435.310 kb on + strand, within IAI47_20935 at 435.311 kb on - strand, within IAI47_20935 at 435.367 kb on + strand, within IAI47_20935 at 435.382 kb on + strand, within IAI47_20935 at 435.382 kb on + strand, within IAI47_20935 at 435.383 kb on - strand, within IAI47_20935 at 435.383 kb on - strand, within IAI47_20935 at 435.430 kb on + strand, within IAI47_20935 at 435.448 kb on + strand, within IAI47_20935 at 435.448 kb on + strand, within IAI47_20935 at 435.448 kb on + strand, within IAI47_20935 at 435.448 kb on + strand, within IAI47_20935 at 435.448 kb on + strand, within IAI47_20935 at 435.449 kb on - strand, within IAI47_20935 at 435.449 kb on - strand, within IAI47_20935 at 435.449 kb on - strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.483 kb on - strand, within IAI47_20935 at 435.483 kb on - strand, within IAI47_20935 at 435.483 kb on - strand, within IAI47_20935 at 435.542 kb on + strand, within IAI47_20935 at 435.543 kb on - strand, within IAI47_20935 at 435.581 kb on + strand, within IAI47_20935 at 435.582 kb on - strand, within IAI47_20935 at 435.582 kb on - strand, within IAI47_20935 at 435.584 kb on + strand, within IAI47_20935 at 435.584 kb on + strand, within IAI47_20935 at 435.585 kb on - strand, within IAI47_20935 at 435.628 kb on + strand, within IAI47_20935 at 435.628 kb on + strand, within IAI47_20935 at 435.628 kb on + strand, within IAI47_20935 at 435.628 kb on + strand, within IAI47_20935 at 435.628 kb on + strand, within IAI47_20935 at 435.629 kb on - strand, within IAI47_20935 at 435.691 kb on - strand, within IAI47_20935 at 435.691 kb on - strand, within IAI47_20935 at 435.783 kb on + strand, within IAI47_20935 at 435.783 kb on + strand, within IAI47_20935 at 435.783 kb on + strand, within IAI47_20935 at 435.784 kb on - strand, within IAI47_20935 at 435.784 kb on - strand, within IAI47_20935 at 435.784 kb on - strand, within IAI47_20935 at 435.857 kb on + strand, within IAI47_20935 at 435.857 kb on + strand, within IAI47_20935 at 435.857 kb on + strand, within IAI47_20935 at 435.857 kb on + strand, within IAI47_20935 at 435.858 kb on - strand, within IAI47_20935 at 435.858 kb on - strand, within IAI47_20935 at 435.858 kb on - strand, within IAI47_20935 at 435.858 kb on - strand, within IAI47_20935 at 435.972 kb on + strand at 435.972 kb on + strand at 435.973 kb on - strand at 435.973 kb on - strand at 435.973 kb on - strand at 436.043 kb on + strand at 436.043 kb on + strand at 436.044 kb on - strand at 436.044 kb on - strand at 436.081 kb on + strand at 436.081 kb on + strand at 436.081 kb on + strand at 436.082 kb on - strand at 436.089 kb on - strand at 436.089 kb on - strand at 436.089 kb on - strand at 436.166 kb on + strand at 436.166 kb on + strand at 436.167 kb on - strand at 436.167 kb on - strand at 436.167 kb on - strand at 436.187 kb on + strand at 436.187 kb on + strand at 436.188 kb on - strand at 436.188 kb on - strand at 436.204 kb on - strand at 436.204 kb on - strand at 436.204 kb on - strand at 436.230 kb on + strand at 436.230 kb on + strand at 436.230 kb on + strand at 436.312 kb on - strand, within IAI47_20940 at 436.312 kb on - strand, within IAI47_20940 at 436.389 kb on + strand, within IAI47_20940 at 436.389 kb on + strand, within IAI47_20940 at 436.389 kb on + strand, within IAI47_20940 at 436.389 kb on + strand, within IAI47_20940 at 436.390 kb on - strand, within IAI47_20940 at 436.391 kb on + strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.425 kb on + strand, within IAI47_20940 at 436.425 kb on + strand, within IAI47_20940 at 436.425 kb on + strand, within IAI47_20940 at 436.426 kb on - strand, within IAI47_20940 at 436.525 kb on + strand, within IAI47_20940 at 436.525 kb on + strand, within IAI47_20940 at 436.525 kb on + strand, within IAI47_20940 at 436.525 kb on + strand, within IAI47_20940 at 436.525 kb on + strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.529 kb on + strand, within IAI47_20940 at 436.529 kb on + strand, within IAI47_20940 at 436.529 kb on + strand, within IAI47_20940 at 436.530 kb on - strand, within IAI47_20940 at 436.530 kb on - strand, within IAI47_20940 at 436.530 kb on - strand, within IAI47_20940 at 436.530 kb on - strand, within IAI47_20940 at 436.530 kb on - strand, within IAI47_20940 at 436.690 kb on + strand at 436.691 kb on - strand at 436.716 kb on + strand at 436.716 kb on + strand at 436.716 kb on + strand at 436.716 kb on + strand at 436.717 kb on - strand at 436.717 kb on - strand at 436.723 kb on + strand at 436.724 kb on - strand at 436.736 kb on - strand at 436.760 kb on + strand at 436.761 kb on - strand at 436.761 kb on - strand at 436.767 kb on + strand at 436.767 kb on + strand at 436.816 kb on + strand at 436.816 kb on + strand at 436.816 kb on + strand at 436.831 kb on + strand at 436.832 kb on - strand at 436.835 kb on + strand at 436.835 kb on + strand at 436.836 kb on - strand at 436.836 kb on - strand at 436.836 kb on - strand at 436.836 kb on - strand at 437.041 kb on - strand, within IAI47_20945 at 437.041 kb on - strand, within IAI47_20945 at 437.058 kb on - strand, within IAI47_20945 at 437.058 kb on - strand, within IAI47_20945 at 437.058 kb on - strand, within IAI47_20945 at 437.065 kb on + strand, within IAI47_20945 at 437.065 kb on + strand, within IAI47_20945 at 437.065 kb on + strand, within IAI47_20945 at 437.066 kb on - strand, within IAI47_20945 at 437.066 kb on - strand, within IAI47_20945 at 437.066 kb on - strand, within IAI47_20945 at 437.071 kb on + strand, within IAI47_20945 at 437.072 kb on - strand, within IAI47_20945 at 437.090 kb on + strand, within IAI47_20945 at 437.091 kb on - strand, within IAI47_20945 at 437.115 kb on + strand, within IAI47_20945 at 437.116 kb on - strand, within IAI47_20945 at 437.116 kb on - strand, within IAI47_20945 at 437.157 kb on - strand at 437.157 kb on - strand at 437.160 kb on + strand at 437.160 kb on + strand at 437.160 kb on + strand at 437.160 kb on + strand at 437.160 kb on + strand at 437.160 kb on + strand at 437.160 kb on + strand at 437.161 kb on - strand at 437.161 kb on - strand at 437.163 kb on - strand at 437.163 kb on - strand at 437.168 kb on - strand at 437.168 kb on - strand at 437.169 kb on + strand at 437.169 kb on + strand at 437.169 kb on + strand at 437.169 kb on + strand at 437.169 kb on + strand at 437.169 kb on + strand at 437.170 kb on - strand at 437.170 kb on - strand at 437.170 kb on - strand at 437.212 kb on - strand at 437.249 kb on + strand at 437.250 kb on - strand at 437.274 kb on + strand at 437.275 kb on - strand at 437.275 kb on - strand at 437.292 kb on + strand at 437.317 kb on - strand at 437.361 kb on + strand at 437.361 kb on + strand at 437.361 kb on + strand at 437.361 kb on + strand at 437.361 kb on + strand at 437.361 kb on + strand at 437.362 kb on - strand at 437.362 kb on - strand at 437.404 kb on - strand at 437.406 kb on + strand at 437.407 kb on - strand at 437.411 kb on + strand at 437.437 kb on - strand at 437.437 kb on - strand at 437.469 kb on - strand at 437.469 kb on - strand at 437.483 kb on + strand at 437.483 kb on + strand at 437.483 kb on + strand at 437.483 kb on + strand at 437.484 kb on - strand at 437.484 kb on - strand at 437.484 kb on - strand at 437.484 kb on - strand at 437.510 kb on + strand at 437.510 kb on + strand at 437.510 kb on + strand at 437.511 kb on - strand at 437.511 kb on - strand at 437.592 kb on + strand at 437.593 kb on - strand at 437.600 kb on - strand, within IAI47_20950 at 437.642 kb on - strand, within IAI47_20950 at 437.724 kb on - strand, within IAI47_20950 at 437.724 kb on - strand, within IAI47_20950
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 435,203 - IAI47_20935 0.25 -1.4 435,223 - IAI47_20935 0.27 +1.4 435,305 - IAI47_20935 0.35 +0.4 435,310 + IAI47_20935 0.35 +2.2 435,311 - IAI47_20935 0.35 +0.5 435,367 + IAI47_20935 0.40 -0.3 435,382 + IAI47_20935 0.41 -0.4 435,382 + IAI47_20935 0.41 +0.8 435,383 - IAI47_20935 0.42 -1.6 435,383 - IAI47_20935 0.42 +0.9 435,430 + IAI47_20935 0.46 -0.4 435,448 + IAI47_20935 0.47 -1.7 435,448 + IAI47_20935 0.47 -0.3 435,448 + IAI47_20935 0.47 +0.1 435,448 + IAI47_20935 0.47 -2.6 435,448 + IAI47_20935 0.47 +0.9 435,449 - IAI47_20935 0.47 +0.6 435,449 - IAI47_20935 0.47 +1.4 435,449 - IAI47_20935 0.47 -0.4 435,482 + IAI47_20935 0.50 +1.0 435,482 + IAI47_20935 0.50 +0.6 435,482 + IAI47_20935 0.50 -0.0 435,482 + IAI47_20935 0.50 -0.4 435,482 + IAI47_20935 0.50 -0.3 435,482 + IAI47_20935 0.50 -0.9 435,483 - IAI47_20935 0.50 +1.2 435,483 - IAI47_20935 0.50 -1.9 435,483 - IAI47_20935 0.50 -2.4 435,542 + IAI47_20935 0.56 -1.7 435,543 - IAI47_20935 0.56 +0.8 435,581 + IAI47_20935 0.59 +3.0 435,582 - IAI47_20935 0.59 +0.0 435,582 - IAI47_20935 0.59 +0.7 435,584 + IAI47_20935 0.60 -0.8 435,584 + IAI47_20935 0.60 +0.3 435,585 - IAI47_20935 0.60 +1.9 435,628 + IAI47_20935 0.63 +0.8 435,628 + IAI47_20935 0.63 -0.4 435,628 + IAI47_20935 0.63 +0.4 435,628 + IAI47_20935 0.63 +0.5 435,628 + IAI47_20935 0.63 +1.9 435,629 - IAI47_20935 0.64 +1.5 435,691 - IAI47_20935 0.69 +0.7 435,691 - IAI47_20935 0.69 +2.6 435,783 + IAI47_20935 0.77 +0.0 435,783 + IAI47_20935 0.77 -2.0 435,783 + IAI47_20935 0.77 -0.2 435,784 - IAI47_20935 0.77 +0.8 435,784 - IAI47_20935 0.77 -0.2 435,784 - IAI47_20935 0.77 -1.3 435,857 + IAI47_20935 0.84 -0.6 435,857 + IAI47_20935 0.84 -0.9 435,857 + IAI47_20935 0.84 +1.3 435,857 + IAI47_20935 0.84 -1.4 435,858 - IAI47_20935 0.84 +0.0 435,858 - IAI47_20935 0.84 -0.2 435,858 - IAI47_20935 0.84 -0.8 435,858 - IAI47_20935 0.84 +0.3 435,972 + -1.4 435,972 + +1.7 435,973 - -1.7 435,973 - -0.7 435,973 - +0.8 436,043 + +0.1 436,043 + +1.7 436,044 - -0.6 436,044 - -0.9 436,081 + -0.2 436,081 + -0.4 436,081 + -2.0 436,082 - -2.3 436,089 - -1.6 436,089 - -0.3 436,089 - +1.2 436,166 + +1.2 436,166 + -0.2 436,167 - -1.0 436,167 - -0.6 436,167 - -0.5 436,187 + -0.2 436,187 + +0.4 436,188 - +0.8 436,188 - +2.5 436,204 - +0.1 436,204 - +1.0 436,204 - +0.8 436,230 + -1.5 436,230 + +0.6 436,230 + -0.6 436,312 - IAI47_20940 0.23 -0.2 436,312 - IAI47_20940 0.23 -0.6 436,389 + IAI47_20940 0.36 -1.9 436,389 + IAI47_20940 0.36 -0.7 436,389 + IAI47_20940 0.36 -1.4 436,389 + IAI47_20940 0.36 -1.2 436,390 - IAI47_20940 0.37 +0.2 436,391 + IAI47_20940 0.37 -0.6 436,392 - IAI47_20940 0.37 +1.4 436,392 - IAI47_20940 0.37 -0.3 436,392 - IAI47_20940 0.37 -0.4 436,392 - IAI47_20940 0.37 +1.8 436,392 - IAI47_20940 0.37 +0.9 436,392 - IAI47_20940 0.37 +3.0 436,425 + IAI47_20940 0.43 +0.9 436,425 + IAI47_20940 0.43 -1.2 436,425 + IAI47_20940 0.43 -2.2 436,426 - IAI47_20940 0.43 +0.9 436,525 + IAI47_20940 0.61 +0.1 436,525 + IAI47_20940 0.61 -1.6 436,525 + IAI47_20940 0.61 -0.3 436,525 + IAI47_20940 0.61 -0.2 436,525 + IAI47_20940 0.61 -0.2 436,526 - IAI47_20940 0.61 -1.2 436,526 - IAI47_20940 0.61 -0.6 436,526 - IAI47_20940 0.61 +0.4 436,526 - IAI47_20940 0.61 -0.9 436,526 - IAI47_20940 0.61 +0.7 436,526 - IAI47_20940 0.61 -1.5 436,526 - IAI47_20940 0.61 +2.8 436,526 - IAI47_20940 0.61 +1.1 436,529 + IAI47_20940 0.61 +0.5 436,529 + IAI47_20940 0.61 +1.3 436,529 + IAI47_20940 0.61 +0.5 436,530 - IAI47_20940 0.62 -0.2 436,530 - IAI47_20940 0.62 -0.1 436,530 - IAI47_20940 0.62 +0.8 436,530 - IAI47_20940 0.62 -0.2 436,530 - IAI47_20940 0.62 -0.6 436,690 + -3.1 436,691 - -1.6 436,716 + -1.7 436,716 + +0.6 436,716 + -0.6 436,716 + -0.3 436,717 - -0.7 436,717 - -0.1 436,723 + -2.5 436,724 - -1.3 436,736 - +3.4 436,760 + -2.0 436,761 - +0.4 436,761 - +0.5 436,767 + -0.5 436,767 + -2.0 436,816 + +0.0 436,816 + -0.6 436,816 + -2.4 436,831 + +0.4 436,832 - -0.2 436,835 + -1.4 436,835 + +2.0 436,836 - -1.7 436,836 - -0.3 436,836 - -1.2 436,836 - +0.8 437,041 - IAI47_20945 0.66 +1.3 437,041 - IAI47_20945 0.66 +2.8 437,058 - IAI47_20945 0.71 +1.6 437,058 - IAI47_20945 0.71 +0.1 437,058 - IAI47_20945 0.71 +1.7 437,065 + IAI47_20945 0.73 -0.4 437,065 + IAI47_20945 0.73 +1.5 437,065 + IAI47_20945 0.73 -1.6 437,066 - IAI47_20945 0.73 -0.5 437,066 - IAI47_20945 0.73 +0.2 437,066 - IAI47_20945 0.73 +1.0 437,071 + IAI47_20945 0.75 -0.9 437,072 - IAI47_20945 0.75 -0.2 437,090 + IAI47_20945 0.80 -1.9 437,091 - IAI47_20945 0.81 +1.0 437,115 + IAI47_20945 0.88 -2.1 437,116 - IAI47_20945 0.88 +1.4 437,116 - IAI47_20945 0.88 -0.9 437,157 - -1.5 437,157 - +1.4 437,160 + +0.9 437,160 + +0.8 437,160 + +0.4 437,160 + -0.2 437,160 + -0.3 437,160 + +0.4 437,160 + +0.6 437,161 - -0.3 437,161 - -0.5 437,163 - -1.0 437,163 - +1.1 437,168 - +2.1 437,168 - -0.6 437,169 + -0.9 437,169 + -1.3 437,169 + -0.6 437,169 + -1.3 437,169 + +0.4 437,169 + +1.6 437,170 - -0.2 437,170 - +0.3 437,170 - +0.0 437,212 - -0.3 437,249 + -1.7 437,250 - +0.5 437,274 + -0.4 437,275 - +1.4 437,275 - -0.9 437,292 + -0.8 437,317 - +1.4 437,361 + -0.2 437,361 + -0.1 437,361 + +1.0 437,361 + -2.2 437,361 + +1.1 437,361 + +2.1 437,362 - +1.3 437,362 - -1.0 437,404 - +0.1 437,406 + +1.8 437,407 - -0.9 437,411 + +0.3 437,437 - +1.3 437,437 - +0.0 437,469 - -0.3 437,469 - -0.4 437,483 + +0.1 437,483 + +1.6 437,483 + +0.2 437,483 + +1.4 437,484 - +0.4 437,484 - -0.9 437,484 - -1.4 437,484 - +3.1 437,510 + +0.2 437,510 + +0.1 437,510 + -3.1 437,511 - -1.7 437,511 - +0.8 437,592 + +2.7 437,593 - -0.3 437,600 - IAI47_20950 0.11 +0.2 437,642 - IAI47_20950 0.21 +1.8 437,724 - IAI47_20950 0.39 -0.7 437,724 - IAI47_20950 0.39 -2.2
Or see this region's nucleotide sequence