Experiment: Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20630 and IAI47_20635 are separated by 126 nucleotides IAI47_20635 and IAI47_20640 are separated by 27 nucleotides IAI47_20640 and IAI47_20645 are separated by 165 nucleotides
IAI47_20630: IAI47_20630 - DUF2534 family protein, at 376,028 to 377,758
_20630
IAI47_20635: IAI47_20635 - pirin family protein, at 377,885 to 378,562
_20635
IAI47_20640: IAI47_20640 - hypothetical protein, at 378,590 to 378,817
_20640
IAI47_20645: IAI47_20645 - YtfJ family protein, at 378,983 to 379,513
_20645
Position (kb) 377
378
379 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 376.981 kb on + strand, within IAI47_20630 at 376.982 kb on - strand, within IAI47_20630 at 376.982 kb on - strand, within IAI47_20630 at 377.244 kb on + strand, within IAI47_20630 at 377.244 kb on + strand, within IAI47_20630 at 377.245 kb on - strand, within IAI47_20630 at 377.245 kb on - strand, within IAI47_20630 at 377.326 kb on + strand, within IAI47_20630 at 377.357 kb on + strand, within IAI47_20630 at 377.388 kb on + strand, within IAI47_20630 at 377.389 kb on - strand, within IAI47_20630 at 377.468 kb on - strand, within IAI47_20630 at 377.515 kb on + strand, within IAI47_20630 at 377.515 kb on + strand, within IAI47_20630 at 377.529 kb on - strand, within IAI47_20630 at 377.529 kb on - strand, within IAI47_20630 at 377.612 kb on + strand at 377.644 kb on + strand at 377.644 kb on + strand at 377.644 kb on + strand at 377.644 kb on + strand at 377.644 kb on + strand at 377.644 kb on + strand at 377.644 kb on + strand at 377.644 kb on + strand at 377.644 kb on + strand at 377.644 kb on + strand at 377.644 kb on + strand at 377.644 kb on + strand at 377.645 kb on - strand at 377.645 kb on - strand at 377.645 kb on - strand at 377.645 kb on - strand at 377.645 kb on - strand at 377.645 kb on - strand at 377.645 kb on - strand at 377.645 kb on - strand at 377.645 kb on - strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.648 kb on + strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.649 kb on - strand at 377.650 kb on + strand at 377.651 kb on - strand at 377.651 kb on - strand at 377.651 kb on - strand at 377.651 kb on - strand at 377.651 kb on - strand at 377.651 kb on - strand at 377.721 kb on - strand at 377.721 kb on - strand at 377.756 kb on + strand at 377.756 kb on + strand at 377.756 kb on + strand at 377.757 kb on - strand at 377.757 kb on - strand at 377.757 kb on - strand at 377.782 kb on - strand at 377.782 kb on - strand at 377.785 kb on - strand at 377.794 kb on - strand at 377.794 kb on - strand at 377.845 kb on + strand at 377.845 kb on + strand at 377.845 kb on + strand at 377.846 kb on - strand at 377.846 kb on - strand at 377.846 kb on - strand at 377.858 kb on - strand at 377.869 kb on + strand at 377.869 kb on + strand at 377.870 kb on - strand at 377.870 kb on - strand at 377.873 kb on + strand at 377.874 kb on - strand at 377.880 kb on + strand at 377.880 kb on + strand at 377.880 kb on + strand at 377.880 kb on + strand at 377.880 kb on + strand at 377.880 kb on + strand at 377.881 kb on - strand at 377.881 kb on - strand at 377.881 kb on - strand at 377.881 kb on - strand at 377.881 kb on - strand at 377.881 kb on - strand at 377.881 kb on - strand at 377.881 kb on - strand at 377.924 kb on + strand at 377.925 kb on - strand at 378.056 kb on + strand, within IAI47_20635 at 378.056 kb on + strand, within IAI47_20635 at 378.159 kb on - strand, within IAI47_20635 at 378.179 kb on + strand, within IAI47_20635 at 378.179 kb on + strand at 378.179 kb on + strand, within IAI47_20635 at 378.180 kb on - strand, within IAI47_20635 at 378.268 kb on + strand, within IAI47_20635 at 378.269 kb on - strand, within IAI47_20635 at 378.269 kb on - strand, within IAI47_20635 at 378.269 kb on - strand, within IAI47_20635 at 378.341 kb on + strand, within IAI47_20635 at 378.341 kb on + strand, within IAI47_20635 at 378.341 kb on + strand, within IAI47_20635 at 378.341 kb on + strand, within IAI47_20635 at 378.341 kb on + strand, within IAI47_20635 at 378.341 kb on + strand, within IAI47_20635 at 378.341 kb on + strand, within IAI47_20635 at 378.341 kb on + strand, within IAI47_20635 at 378.342 kb on - strand, within IAI47_20635 at 378.342 kb on - strand, within IAI47_20635 at 378.342 kb on - strand, within IAI47_20635 at 378.342 kb on - strand, within IAI47_20635 at 378.342 kb on - strand, within IAI47_20635 at 378.342 kb on - strand, within IAI47_20635 at 378.342 kb on - strand, within IAI47_20635 at 378.342 kb on - strand, within IAI47_20635 at 378.342 kb on - strand, within IAI47_20635 at 378.342 kb on - strand, within IAI47_20635 at 378.342 kb on - strand, within IAI47_20635 at 378.343 kb on + strand, within IAI47_20635 at 378.343 kb on + strand, within IAI47_20635 at 378.343 kb on + strand, within IAI47_20635 at 378.343 kb on + strand, within IAI47_20635 at 378.343 kb on + strand, within IAI47_20635 at 378.344 kb on - strand, within IAI47_20635 at 378.344 kb on - strand, within IAI47_20635 at 378.344 kb on - strand, within IAI47_20635 at 378.344 kb on - strand, within IAI47_20635 at 378.344 kb on - strand, within IAI47_20635 at 378.344 kb on - strand, within IAI47_20635 at 378.344 kb on - strand, within IAI47_20635 at 378.344 kb on - strand, within IAI47_20635 at 378.347 kb on + strand, within IAI47_20635 at 378.347 kb on + strand, within IAI47_20635 at 378.347 kb on + strand, within IAI47_20635 at 378.348 kb on - strand, within IAI47_20635 at 378.348 kb on - strand, within IAI47_20635 at 378.348 kb on - strand, within IAI47_20635 at 378.348 kb on - strand, within IAI47_20635 at 378.373 kb on + strand, within IAI47_20635 at 378.373 kb on + strand, within IAI47_20635 at 378.374 kb on - strand, within IAI47_20635 at 378.374 kb on - strand, within IAI47_20635 at 378.378 kb on + strand, within IAI47_20635 at 378.378 kb on + strand, within IAI47_20635 at 378.410 kb on + strand, within IAI47_20635 at 378.411 kb on - strand, within IAI47_20635 at 378.411 kb on - strand, within IAI47_20635 at 378.520 kb on + strand at 378.602 kb on - strand at 378.602 kb on - strand at 378.630 kb on + strand, within IAI47_20640 at 378.630 kb on + strand, within IAI47_20640 at 378.630 kb on + strand, within IAI47_20640 at 378.630 kb on + strand, within IAI47_20640 at 378.631 kb on - strand, within IAI47_20640 at 378.631 kb on - strand, within IAI47_20640 at 378.631 kb on - strand, within IAI47_20640 at 378.631 kb on - strand, within IAI47_20640 at 378.775 kb on + strand, within IAI47_20640 at 378.775 kb on + strand, within IAI47_20640 at 378.775 kb on + strand, within IAI47_20640 at 378.775 kb on + strand, within IAI47_20640 at 378.776 kb on - strand, within IAI47_20640 at 378.776 kb on - strand, within IAI47_20640 at 378.776 kb on - strand, within IAI47_20640 at 378.776 kb on - strand, within IAI47_20640 at 378.776 kb on - strand, within IAI47_20640 at 378.777 kb on + strand, within IAI47_20640 at 378.778 kb on - strand, within IAI47_20640 at 378.794 kb on + strand, within IAI47_20640 at 378.795 kb on - strand at 378.811 kb on + strand at 378.812 kb on - strand at 378.831 kb on + strand at 378.867 kb on + strand at 378.868 kb on - strand at 378.892 kb on + strand at 378.892 kb on + strand at 378.893 kb on - strand at 378.893 kb on - strand at 378.901 kb on + strand at 378.904 kb on + strand at 378.904 kb on + strand at 378.904 kb on + strand at 378.905 kb on - strand at 378.905 kb on - strand at 378.905 kb on - strand at 378.927 kb on + strand at 378.927 kb on + strand at 378.927 kb on + strand at 378.927 kb on + strand at 378.927 kb on + strand at 378.927 kb on + strand at 378.928 kb on - strand at 378.928 kb on - strand at 378.928 kb on - strand at 378.928 kb on - strand at 378.936 kb on + strand at 378.936 kb on + strand at 378.937 kb on - strand at 378.937 kb on - strand at 378.940 kb on + strand at 378.940 kb on + strand at 378.940 kb on + strand at 378.941 kb on - strand at 378.941 kb on - strand at 378.941 kb on - strand at 378.988 kb on - strand at 378.988 kb on - strand at 379.055 kb on + strand, within IAI47_20645 at 379.055 kb on + strand, within IAI47_20645 at 379.055 kb on + strand, within IAI47_20645 at 379.055 kb on + strand, within IAI47_20645 at 379.055 kb on + strand, within IAI47_20645 at 379.056 kb on - strand, within IAI47_20645 at 379.056 kb on - strand, within IAI47_20645 at 379.056 kb on - strand, within IAI47_20645 at 379.103 kb on + strand, within IAI47_20645 at 379.104 kb on - strand, within IAI47_20645 at 379.197 kb on + strand, within IAI47_20645 at 379.197 kb on + strand, within IAI47_20645 at 379.197 kb on + strand, within IAI47_20645 at 379.262 kb on - strand, within IAI47_20645 at 379.297 kb on - strand, within IAI47_20645 at 379.303 kb on + strand, within IAI47_20645 at 379.303 kb on + strand, within IAI47_20645 at 379.303 kb on + strand, within IAI47_20645 at 379.303 kb on + strand, within IAI47_20645 at 379.304 kb on - strand, within IAI47_20645 at 379.304 kb on - strand, within IAI47_20645 at 379.304 kb on - strand, within IAI47_20645 at 379.304 kb on - strand, within IAI47_20645 at 379.304 kb on - strand, within IAI47_20645 at 379.349 kb on - strand, within IAI47_20645 at 379.458 kb on + strand, within IAI47_20645 at 379.458 kb on + strand, within IAI47_20645 at 379.458 kb on + strand, within IAI47_20645 at 379.459 kb on - strand, within IAI47_20645 at 379.533 kb on + strand at 379.533 kb on + strand at 379.533 kb on + strand at 379.534 kb on - strand at 379.538 kb on - strand at 379.538 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 376,981 + IAI47_20630 0.55 +0.4 376,982 - IAI47_20630 0.55 +0.2 376,982 - IAI47_20630 0.55 +0.2 377,244 + IAI47_20630 0.70 -1.4 377,244 + IAI47_20630 0.70 +0.0 377,245 - IAI47_20630 0.70 -0.2 377,245 - IAI47_20630 0.70 +0.7 377,326 + IAI47_20630 0.75 +1.5 377,357 + IAI47_20630 0.77 +0.5 377,388 + IAI47_20630 0.79 -0.2 377,389 - IAI47_20630 0.79 -1.8 377,468 - IAI47_20630 0.83 +2.2 377,515 + IAI47_20630 0.86 -1.9 377,515 + IAI47_20630 0.86 +0.3 377,529 - IAI47_20630 0.87 +0.7 377,529 - IAI47_20630 0.87 -0.3 377,612 + -0.2 377,644 + -1.1 377,644 + -0.9 377,644 + -1.9 377,644 + -0.8 377,644 + -0.9 377,644 + -0.8 377,644 + +2.2 377,644 + -2.1 377,644 + -1.5 377,644 + +1.6 377,644 + +0.6 377,644 + +0.2 377,645 - -1.6 377,645 - -2.4 377,645 - +0.8 377,645 - +1.8 377,645 - -1.4 377,645 - -0.6 377,645 - -1.3 377,645 - +0.0 377,645 - -1.3 377,648 + -0.6 377,648 + +2.2 377,648 + -1.5 377,648 + +2.5 377,648 + -0.4 377,648 + +1.0 377,648 + -2.9 377,648 + +0.2 377,648 + -0.8 377,648 + +0.8 377,648 + -1.8 377,648 + -1.9 377,648 + -0.4 377,648 + -2.7 377,648 + -0.4 377,648 + +3.5 377,649 - -0.0 377,649 - +0.6 377,649 - +0.2 377,649 - -2.5 377,649 - +1.2 377,649 - +0.9 377,649 - -2.4 377,649 - -2.7 377,649 - -0.8 377,649 - +0.9 377,649 - -0.6 377,649 - -0.7 377,649 - -0.1 377,649 - +0.9 377,649 - +0.2 377,649 - +1.4 377,649 - -1.9 377,649 - -2.6 377,650 + +1.2 377,651 - -1.1 377,651 - -0.4 377,651 - +2.4 377,651 - -0.5 377,651 - -0.8 377,651 - -0.7 377,721 - +2.2 377,721 - +0.3 377,756 + +1.8 377,756 + -1.4 377,756 + -1.2 377,757 - -1.4 377,757 - +2.2 377,757 - -0.4 377,782 - +0.7 377,782 - -0.9 377,785 - +0.4 377,794 - +0.1 377,794 - +2.2 377,845 + -2.5 377,845 + -2.2 377,845 + +1.1 377,846 - -0.1 377,846 - +2.5 377,846 - -2.7 377,858 - -1.6 377,869 + +2.3 377,869 + +0.2 377,870 - -2.1 377,870 - +0.1 377,873 + +1.9 377,874 - -0.5 377,880 + +0.5 377,880 + +0.7 377,880 + -0.0 377,880 + +0.8 377,880 + -2.1 377,880 + +0.2 377,881 - +1.2 377,881 - -0.4 377,881 - +0.2 377,881 - -0.3 377,881 - -1.4 377,881 - +1.2 377,881 - +0.0 377,881 - -2.2 377,924 + +0.7 377,925 - -2.2 378,056 + IAI47_20635 0.25 -0.1 378,056 + IAI47_20635 0.25 -2.5 378,159 - IAI47_20635 0.40 -0.0 378,179 + IAI47_20635 0.43 -0.4 378,179 + +1.2 378,179 + IAI47_20635 0.43 -0.4 378,180 - IAI47_20635 0.44 -0.4 378,268 + IAI47_20635 0.56 +0.9 378,269 - IAI47_20635 0.57 -0.3 378,269 - IAI47_20635 0.57 +0.2 378,269 - IAI47_20635 0.57 -0.8 378,341 + IAI47_20635 0.67 -2.5 378,341 + IAI47_20635 0.67 +0.6 378,341 + IAI47_20635 0.67 +1.8 378,341 + IAI47_20635 0.67 -2.5 378,341 + IAI47_20635 0.67 -0.6 378,341 + IAI47_20635 0.67 +1.0 378,341 + IAI47_20635 0.67 -0.1 378,341 + IAI47_20635 0.67 +1.0 378,342 - IAI47_20635 0.67 +0.2 378,342 - IAI47_20635 0.67 -0.8 378,342 - IAI47_20635 0.67 +0.1 378,342 - IAI47_20635 0.67 +2.2 378,342 - IAI47_20635 0.67 -1.0 378,342 - IAI47_20635 0.67 -0.8 378,342 - IAI47_20635 0.67 +1.7 378,342 - IAI47_20635 0.67 +2.0 378,342 - IAI47_20635 0.67 -2.9 378,342 - IAI47_20635 0.67 +0.1 378,342 - IAI47_20635 0.67 +2.6 378,343 + IAI47_20635 0.68 +3.0 378,343 + IAI47_20635 0.68 -2.4 378,343 + IAI47_20635 0.68 +0.8 378,343 + IAI47_20635 0.68 -1.8 378,343 + IAI47_20635 0.68 +0.5 378,344 - IAI47_20635 0.68 +0.2 378,344 - IAI47_20635 0.68 -1.6 378,344 - IAI47_20635 0.68 +0.8 378,344 - IAI47_20635 0.68 -1.4 378,344 - IAI47_20635 0.68 +0.9 378,344 - IAI47_20635 0.68 +1.6 378,344 - IAI47_20635 0.68 +2.0 378,344 - IAI47_20635 0.68 +2.2 378,347 + IAI47_20635 0.68 +1.2 378,347 + IAI47_20635 0.68 +0.8 378,347 + IAI47_20635 0.68 -0.3 378,348 - IAI47_20635 0.68 +1.2 378,348 - IAI47_20635 0.68 +0.3 378,348 - IAI47_20635 0.68 -0.8 378,348 - IAI47_20635 0.68 -0.4 378,373 + IAI47_20635 0.72 -0.8 378,373 + IAI47_20635 0.72 -1.9 378,374 - IAI47_20635 0.72 +0.6 378,374 - IAI47_20635 0.72 -0.0 378,378 + IAI47_20635 0.73 +0.6 378,378 + IAI47_20635 0.73 -0.8 378,410 + IAI47_20635 0.77 +2.1 378,411 - IAI47_20635 0.78 -0.2 378,411 - IAI47_20635 0.78 -0.2 378,520 + +1.8 378,602 - -0.8 378,602 - +1.6 378,630 + IAI47_20640 0.18 +0.6 378,630 + IAI47_20640 0.18 +1.9 378,630 + IAI47_20640 0.18 +1.2 378,630 + IAI47_20640 0.18 -1.4 378,631 - IAI47_20640 0.18 -1.3 378,631 - IAI47_20640 0.18 -1.1 378,631 - IAI47_20640 0.18 -0.4 378,631 - IAI47_20640 0.18 +0.9 378,775 + IAI47_20640 0.81 +0.1 378,775 + IAI47_20640 0.81 +0.5 378,775 + IAI47_20640 0.81 -1.4 378,775 + IAI47_20640 0.81 -1.2 378,776 - IAI47_20640 0.82 +0.5 378,776 - IAI47_20640 0.82 +1.8 378,776 - IAI47_20640 0.82 -1.4 378,776 - IAI47_20640 0.82 -0.8 378,776 - IAI47_20640 0.82 -1.4 378,777 + IAI47_20640 0.82 +0.3 378,778 - IAI47_20640 0.82 +0.6 378,794 + IAI47_20640 0.89 +0.2 378,795 - -2.6 378,811 + -0.4 378,812 - +0.2 378,831 + +2.2 378,867 + -1.2 378,868 - -1.4 378,892 + -2.1 378,892 + -1.4 378,893 - -0.4 378,893 - -0.4 378,901 + +1.4 378,904 + -1.9 378,904 + -1.2 378,904 + -0.8 378,905 - +1.2 378,905 - +0.6 378,905 - +0.7 378,927 + +1.3 378,927 + +0.6 378,927 + +0.9 378,927 + +3.0 378,927 + -0.9 378,927 + +0.0 378,928 - -0.3 378,928 - +0.1 378,928 - +0.6 378,928 - -1.4 378,936 + -2.7 378,936 + -1.4 378,937 - -1.7 378,937 - -1.4 378,940 + -2.7 378,940 + +0.4 378,940 + +2.2 378,941 - -1.0 378,941 - -1.5 378,941 - -2.6 378,988 - +1.2 378,988 - +1.5 379,055 + IAI47_20645 0.14 +0.2 379,055 + IAI47_20645 0.14 -1.1 379,055 + IAI47_20645 0.14 -0.9 379,055 + IAI47_20645 0.14 +0.4 379,055 + IAI47_20645 0.14 +1.3 379,056 - IAI47_20645 0.14 +0.6 379,056 - IAI47_20645 0.14 -3.2 379,056 - IAI47_20645 0.14 -1.4 379,103 + IAI47_20645 0.23 +0.2 379,104 - IAI47_20645 0.23 -1.2 379,197 + IAI47_20645 0.40 -0.8 379,197 + IAI47_20645 0.40 -2.5 379,197 + IAI47_20645 0.40 +2.8 379,262 - IAI47_20645 0.53 -2.6 379,297 - IAI47_20645 0.59 +2.6 379,303 + IAI47_20645 0.60 -2.1 379,303 + IAI47_20645 0.60 +3.8 379,303 + IAI47_20645 0.60 +0.3 379,303 + IAI47_20645 0.60 +1.7 379,304 - IAI47_20645 0.60 -0.2 379,304 - IAI47_20645 0.60 -1.6 379,304 - IAI47_20645 0.60 +0.1 379,304 - IAI47_20645 0.60 -1.1 379,304 - IAI47_20645 0.60 -0.2 379,349 - IAI47_20645 0.69 +0.1 379,458 + IAI47_20645 0.89 -0.3 379,458 + IAI47_20645 0.89 -0.2 379,458 + IAI47_20645 0.89 +1.0 379,459 - IAI47_20645 0.90 -2.9 379,533 + -0.8 379,533 + -2.8 379,533 + -0.4 379,534 - -1.3 379,538 - +1.7 379,538 - +1.9
Or see this region's nucleotide sequence