Experiment: Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20120 and IAI47_20125 are separated by 357 nucleotides IAI47_20125 and IAI47_20130 are separated by 44 nucleotides IAI47_20130 and IAI47_20135 are separated by 51 nucleotides
IAI47_20120: IAI47_20120 - transcriptional regulator, at 257,745 to 258,515
_20120
IAI47_20125: IAI47_20125 - hypothetical protein, at 258,873 to 259,268
_20125
IAI47_20130: IAI47_20130 - nuclear transport factor 2 family protein, at 259,313 to 259,786
_20130
IAI47_20135: IAI47_20135 - 6,7-dimethyl-8-ribityllumazine synthase, at 259,838 to 260,272
_20135
Position (kb) 258
259
260 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 257.909 kb on + strand, within IAI47_20120 at 257.909 kb on + strand, within IAI47_20120 at 257.909 kb on + strand, within IAI47_20120 at 257.910 kb on - strand, within IAI47_20120 at 257.910 kb on - strand, within IAI47_20120 at 257.910 kb on - strand, within IAI47_20120 at 257.910 kb on - strand, within IAI47_20120 at 257.910 kb on - strand, within IAI47_20120 at 257.951 kb on + strand, within IAI47_20120 at 257.951 kb on + strand, within IAI47_20120 at 257.951 kb on + strand, within IAI47_20120 at 257.952 kb on - strand, within IAI47_20120 at 257.952 kb on - strand, within IAI47_20120 at 257.967 kb on - strand, within IAI47_20120 at 257.967 kb on - strand, within IAI47_20120 at 257.999 kb on + strand, within IAI47_20120 at 257.999 kb on + strand, within IAI47_20120 at 258.027 kb on + strand, within IAI47_20120 at 258.027 kb on + strand, within IAI47_20120 at 258.028 kb on - strand, within IAI47_20120 at 258.045 kb on - strand, within IAI47_20120 at 258.045 kb on - strand, within IAI47_20120 at 258.045 kb on - strand, within IAI47_20120 at 258.071 kb on + strand, within IAI47_20120 at 258.171 kb on - strand, within IAI47_20120 at 258.196 kb on + strand, within IAI47_20120 at 258.196 kb on + strand, within IAI47_20120 at 258.197 kb on - strand, within IAI47_20120 at 258.197 kb on - strand, within IAI47_20120 at 258.197 kb on - strand, within IAI47_20120 at 258.197 kb on - strand, within IAI47_20120 at 258.292 kb on + strand, within IAI47_20120 at 258.292 kb on + strand, within IAI47_20120 at 258.292 kb on + strand, within IAI47_20120 at 258.292 kb on + strand, within IAI47_20120 at 258.292 kb on + strand, within IAI47_20120 at 258.292 kb on + strand, within IAI47_20120 at 258.293 kb on - strand, within IAI47_20120 at 258.293 kb on - strand, within IAI47_20120 at 258.293 kb on - strand, within IAI47_20120 at 258.293 kb on - strand, within IAI47_20120 at 258.293 kb on - strand, within IAI47_20120 at 258.293 kb on - strand, within IAI47_20120 at 258.360 kb on + strand, within IAI47_20120 at 258.361 kb on - strand, within IAI47_20120 at 258.369 kb on + strand, within IAI47_20120 at 258.369 kb on + strand, within IAI47_20120 at 258.369 kb on + strand, within IAI47_20120 at 258.370 kb on - strand, within IAI47_20120 at 258.370 kb on - strand, within IAI47_20120 at 258.370 kb on - strand, within IAI47_20120 at 258.376 kb on + strand, within IAI47_20120 at 258.444 kb on - strand at 258.444 kb on - strand at 258.455 kb on + strand at 258.455 kb on + strand at 258.456 kb on - strand at 258.456 kb on - strand at 258.456 kb on - strand at 258.461 kb on + strand at 258.491 kb on + strand at 258.491 kb on + strand at 258.492 kb on - strand at 258.495 kb on - strand at 258.495 kb on - strand at 258.503 kb on + strand at 258.516 kb on - strand at 258.516 kb on - strand at 258.516 kb on - strand at 258.516 kb on - strand at 258.565 kb on + strand at 258.565 kb on + strand at 258.565 kb on + strand at 258.565 kb on + strand at 258.565 kb on + strand at 258.566 kb on - strand at 258.566 kb on - strand at 258.566 kb on - strand at 258.566 kb on - strand at 258.566 kb on - strand at 258.566 kb on - strand at 258.569 kb on + strand at 258.569 kb on + strand at 258.569 kb on + strand at 258.569 kb on + strand at 258.569 kb on + strand at 258.569 kb on + strand at 258.569 kb on + strand at 258.569 kb on + strand at 258.569 kb on + strand at 258.569 kb on + strand at 258.570 kb on - strand at 258.570 kb on - strand at 258.570 kb on - strand at 258.570 kb on - strand at 258.570 kb on - strand at 258.570 kb on - strand at 258.570 kb on - strand at 258.570 kb on - strand at 258.570 kb on - strand at 258.572 kb on + strand at 258.572 kb on + strand at 258.572 kb on + strand at 258.573 kb on - strand at 258.573 kb on - strand at 258.573 kb on - strand at 258.573 kb on - strand at 258.573 kb on - strand at 258.581 kb on + strand at 258.581 kb on + strand at 258.581 kb on + strand at 258.581 kb on + strand at 258.581 kb on + strand at 258.582 kb on - strand at 258.582 kb on - strand at 258.582 kb on - strand at 258.582 kb on - strand at 258.582 kb on - strand at 258.582 kb on - strand at 258.584 kb on - strand at 258.584 kb on - strand at 258.595 kb on - strand at 258.647 kb on - strand at 258.690 kb on + strand at 258.690 kb on + strand at 258.725 kb on - strand at 258.728 kb on + strand at 258.729 kb on - strand at 258.746 kb on + strand at 258.747 kb on - strand at 258.754 kb on + strand at 258.804 kb on + strand at 258.812 kb on + strand at 258.812 kb on + strand at 258.812 kb on + strand at 258.813 kb on - strand at 258.813 kb on - strand at 258.813 kb on - strand at 258.813 kb on - strand at 258.834 kb on - strand at 258.887 kb on + strand at 258.888 kb on - strand at 258.888 kb on - strand at 258.916 kb on + strand, within IAI47_20125 at 258.916 kb on + strand, within IAI47_20125 at 258.916 kb on + strand, within IAI47_20125 at 259.042 kb on + strand, within IAI47_20125 at 259.042 kb on + strand, within IAI47_20125 at 259.042 kb on + strand, within IAI47_20125 at 259.042 kb on + strand, within IAI47_20125 at 259.043 kb on - strand, within IAI47_20125 at 259.043 kb on - strand, within IAI47_20125 at 259.066 kb on + strand, within IAI47_20125 at 259.067 kb on - strand, within IAI47_20125 at 259.067 kb on - strand, within IAI47_20125 at 259.067 kb on - strand, within IAI47_20125 at 259.067 kb on - strand, within IAI47_20125 at 259.067 kb on - strand, within IAI47_20125 at 259.070 kb on + strand, within IAI47_20125 at 259.070 kb on + strand, within IAI47_20125 at 259.070 kb on + strand, within IAI47_20125 at 259.071 kb on - strand, within IAI47_20125 at 259.125 kb on + strand, within IAI47_20125 at 259.125 kb on + strand, within IAI47_20125 at 259.145 kb on + strand, within IAI47_20125 at 259.146 kb on - strand, within IAI47_20125 at 259.146 kb on - strand, within IAI47_20125 at 259.146 kb on - strand, within IAI47_20125 at 259.171 kb on + strand, within IAI47_20125 at 259.171 kb on + strand, within IAI47_20125 at 259.171 kb on + strand, within IAI47_20125 at 259.172 kb on - strand, within IAI47_20125 at 259.172 kb on - strand, within IAI47_20125 at 259.212 kb on + strand, within IAI47_20125 at 259.212 kb on + strand, within IAI47_20125 at 259.213 kb on - strand, within IAI47_20125 at 259.221 kb on - strand, within IAI47_20125 at 259.250 kb on + strand at 259.250 kb on + strand at 259.251 kb on - strand at 259.251 kb on - strand at 259.314 kb on + strand at 259.315 kb on - strand at 259.315 kb on - strand at 259.316 kb on + strand at 259.316 kb on + strand at 259.317 kb on - strand at 259.317 kb on - strand at 259.400 kb on - strand, within IAI47_20130 at 259.433 kb on + strand, within IAI47_20130 at 259.433 kb on + strand, within IAI47_20130 at 259.434 kb on - strand, within IAI47_20130 at 259.470 kb on + strand, within IAI47_20130 at 259.470 kb on + strand, within IAI47_20130 at 259.471 kb on - strand, within IAI47_20130 at 259.471 kb on - strand, within IAI47_20130 at 259.471 kb on - strand, within IAI47_20130 at 259.471 kb on - strand, within IAI47_20130 at 259.480 kb on - strand, within IAI47_20130 at 259.509 kb on + strand, within IAI47_20130 at 259.510 kb on - strand, within IAI47_20130 at 259.510 kb on - strand, within IAI47_20130 at 259.558 kb on + strand, within IAI47_20130 at 259.558 kb on + strand, within IAI47_20130 at 259.559 kb on - strand, within IAI47_20130 at 259.559 kb on - strand, within IAI47_20130 at 259.646 kb on - strand, within IAI47_20130 at 259.668 kb on + strand, within IAI47_20130 at 259.669 kb on - strand, within IAI47_20130 at 259.801 kb on - strand at 259.801 kb on - strand at 259.909 kb on + strand, within IAI47_20135 at 259.909 kb on + strand, within IAI47_20135 at 259.909 kb on + strand, within IAI47_20135 at 259.909 kb on + strand, within IAI47_20135 at 259.910 kb on - strand, within IAI47_20135 at 259.910 kb on - strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.911 kb on + strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.912 kb on - strand, within IAI47_20135 at 259.945 kb on + strand, within IAI47_20135 at 259.946 kb on - strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.043 kb on + strand, within IAI47_20135 at 260.044 kb on - strand, within IAI47_20135 at 260.044 kb on - strand, within IAI47_20135 at 260.044 kb on - strand, within IAI47_20135 at 260.044 kb on - strand, within IAI47_20135 at 260.057 kb on + strand, within IAI47_20135 at 260.057 kb on + strand, within IAI47_20135 at 260.057 kb on + strand, within IAI47_20135 at 260.057 kb on + strand, within IAI47_20135 at 260.058 kb on - strand, within IAI47_20135 at 260.058 kb on - strand, within IAI47_20135 at 260.058 kb on - strand, within IAI47_20135 at 260.058 kb on - strand, within IAI47_20135 at 260.120 kb on + strand, within IAI47_20135 at 260.166 kb on + strand, within IAI47_20135 at 260.166 kb on + strand, within IAI47_20135 at 260.166 kb on + strand, within IAI47_20135 at 260.167 kb on - strand, within IAI47_20135 at 260.167 kb on - strand, within IAI47_20135 at 260.245 kb on + strand at 260.245 kb on + strand at 260.246 kb on - strand at 260.248 kb on + strand at 260.248 kb on + strand at 260.249 kb on - strand at 260.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 257,909 + IAI47_20120 0.21 +0.0 257,909 + IAI47_20120 0.21 +1.9 257,909 + IAI47_20120 0.21 +1.3 257,910 - IAI47_20120 0.21 +2.0 257,910 - IAI47_20120 0.21 -1.6 257,910 - IAI47_20120 0.21 -0.6 257,910 - IAI47_20120 0.21 -0.5 257,910 - IAI47_20120 0.21 -0.7 257,951 + IAI47_20120 0.27 -1.6 257,951 + IAI47_20120 0.27 +0.6 257,951 + IAI47_20120 0.27 -2.0 257,952 - IAI47_20120 0.27 +0.2 257,952 - IAI47_20120 0.27 -0.1 257,967 - IAI47_20120 0.29 +0.2 257,967 - IAI47_20120 0.29 -0.4 257,999 + IAI47_20120 0.33 +1.2 257,999 + IAI47_20120 0.33 -2.0 258,027 + IAI47_20120 0.37 -0.2 258,027 + IAI47_20120 0.37 +0.2 258,028 - IAI47_20120 0.37 +1.2 258,045 - IAI47_20120 0.39 -0.4 258,045 - IAI47_20120 0.39 -1.8 258,045 - IAI47_20120 0.39 -3.2 258,071 + IAI47_20120 0.42 +0.2 258,171 - IAI47_20120 0.55 +0.9 258,196 + IAI47_20120 0.58 -1.3 258,196 + IAI47_20120 0.58 +0.6 258,197 - IAI47_20120 0.59 +0.2 258,197 - IAI47_20120 0.59 +1.9 258,197 - IAI47_20120 0.59 +0.9 258,197 - IAI47_20120 0.59 +1.7 258,292 + IAI47_20120 0.71 -2.3 258,292 + IAI47_20120 0.71 -0.8 258,292 + IAI47_20120 0.71 +0.2 258,292 + IAI47_20120 0.71 -0.8 258,292 + IAI47_20120 0.71 +2.0 258,292 + IAI47_20120 0.71 -2.7 258,293 - IAI47_20120 0.71 +0.8 258,293 - IAI47_20120 0.71 -1.6 258,293 - IAI47_20120 0.71 -2.3 258,293 - IAI47_20120 0.71 -0.6 258,293 - IAI47_20120 0.71 -0.6 258,293 - IAI47_20120 0.71 -0.1 258,360 + IAI47_20120 0.80 +0.9 258,361 - IAI47_20120 0.80 +2.3 258,369 + IAI47_20120 0.81 -0.4 258,369 + IAI47_20120 0.81 -0.9 258,369 + IAI47_20120 0.81 +3.2 258,370 - IAI47_20120 0.81 +0.5 258,370 - IAI47_20120 0.81 -0.6 258,370 - IAI47_20120 0.81 -1.6 258,376 + IAI47_20120 0.82 +2.0 258,444 - +0.8 258,444 - -0.4 258,455 + +1.5 258,455 + +0.1 258,456 - +1.2 258,456 - -1.8 258,456 - +1.2 258,461 + -3.3 258,491 + -2.0 258,491 + -0.3 258,492 - -0.8 258,495 - -0.5 258,495 - -0.1 258,503 + -0.2 258,516 - -0.1 258,516 - +2.6 258,516 - +0.6 258,516 - -0.8 258,565 + +0.2 258,565 + +0.2 258,565 + -3.3 258,565 + +0.4 258,565 + -0.4 258,566 - +1.6 258,566 - +1.9 258,566 - +0.1 258,566 - -0.6 258,566 - -1.1 258,566 - -0.1 258,569 + -2.4 258,569 + -1.8 258,569 + -0.8 258,569 + -0.6 258,569 + +1.4 258,569 + -0.0 258,569 + -1.2 258,569 + +2.3 258,569 + -1.4 258,569 + -2.1 258,570 - +0.3 258,570 - +1.0 258,570 - +1.2 258,570 - -0.1 258,570 - +2.7 258,570 - -0.4 258,570 - +2.0 258,570 - -2.5 258,570 - +2.9 258,572 + +2.5 258,572 + -1.4 258,572 + -1.8 258,573 - +0.2 258,573 - -1.4 258,573 - -2.6 258,573 - -0.4 258,573 - +0.7 258,581 + +2.5 258,581 + -0.6 258,581 + -0.8 258,581 + +0.2 258,581 + +0.0 258,582 - +0.8 258,582 - -2.0 258,582 - -1.2 258,582 - -1.6 258,582 - -1.6 258,582 - +3.4 258,584 - +0.1 258,584 - -0.1 258,595 - -0.1 258,647 - +0.4 258,690 + -3.2 258,690 + +1.6 258,725 - -1.6 258,728 + -2.1 258,729 - +0.2 258,746 + -0.4 258,747 - +0.6 258,754 + +1.3 258,804 + -0.4 258,812 + -1.8 258,812 + -0.0 258,812 + +1.3 258,813 - -0.4 258,813 - +2.0 258,813 - +1.2 258,813 - -1.1 258,834 - -1.2 258,887 + -1.0 258,888 - -1.7 258,888 - +0.5 258,916 + IAI47_20125 0.11 -0.3 258,916 + IAI47_20125 0.11 -0.4 258,916 + IAI47_20125 0.11 -0.1 259,042 + IAI47_20125 0.43 +0.2 259,042 + IAI47_20125 0.43 -3.7 259,042 + IAI47_20125 0.43 -0.8 259,042 + IAI47_20125 0.43 -1.6 259,043 - IAI47_20125 0.43 +0.6 259,043 - IAI47_20125 0.43 +0.9 259,066 + IAI47_20125 0.49 -2.1 259,067 - IAI47_20125 0.49 -0.3 259,067 - IAI47_20125 0.49 -1.4 259,067 - IAI47_20125 0.49 -0.6 259,067 - IAI47_20125 0.49 -0.9 259,067 - IAI47_20125 0.49 -0.2 259,070 + IAI47_20125 0.50 +0.2 259,070 + IAI47_20125 0.50 -1.1 259,070 + IAI47_20125 0.50 -1.0 259,071 - IAI47_20125 0.50 -2.1 259,125 + IAI47_20125 0.64 -0.6 259,125 + IAI47_20125 0.64 +0.9 259,145 + IAI47_20125 0.69 -0.0 259,146 - IAI47_20125 0.69 -2.0 259,146 - IAI47_20125 0.69 -0.9 259,146 - IAI47_20125 0.69 -0.4 259,171 + IAI47_20125 0.75 -2.4 259,171 + IAI47_20125 0.75 -0.2 259,171 + IAI47_20125 0.75 -0.4 259,172 - IAI47_20125 0.76 +0.5 259,172 - IAI47_20125 0.76 +1.2 259,212 + IAI47_20125 0.86 -1.8 259,212 + IAI47_20125 0.86 +0.9 259,213 - IAI47_20125 0.86 -2.0 259,221 - IAI47_20125 0.88 +0.2 259,250 + -1.7 259,250 + +1.5 259,251 - +1.2 259,251 - +0.5 259,314 + -1.5 259,315 - -1.1 259,315 - -1.4 259,316 + -2.4 259,316 + +0.0 259,317 - -0.2 259,317 - +3.0 259,400 - IAI47_20130 0.18 -1.1 259,433 + IAI47_20130 0.25 -2.8 259,433 + IAI47_20130 0.25 +0.4 259,434 - IAI47_20130 0.26 +1.1 259,470 + IAI47_20130 0.33 -0.7 259,470 + IAI47_20130 0.33 +1.6 259,471 - IAI47_20130 0.33 -1.1 259,471 - IAI47_20130 0.33 -0.2 259,471 - IAI47_20130 0.33 +1.0 259,471 - IAI47_20130 0.33 +0.9 259,480 - IAI47_20130 0.35 -2.1 259,509 + IAI47_20130 0.41 +1.0 259,510 - IAI47_20130 0.42 -0.8 259,510 - IAI47_20130 0.42 +0.7 259,558 + IAI47_20130 0.52 -0.7 259,558 + IAI47_20130 0.52 +1.5 259,559 - IAI47_20130 0.52 +0.2 259,559 - IAI47_20130 0.52 +1.0 259,646 - IAI47_20130 0.70 +2.1 259,668 + IAI47_20130 0.75 +1.9 259,669 - IAI47_20130 0.75 -1.6 259,801 - -0.2 259,801 - +2.0 259,909 + IAI47_20135 0.16 -0.3 259,909 + IAI47_20135 0.16 +0.4 259,909 + IAI47_20135 0.16 -0.5 259,909 + IAI47_20135 0.16 -2.3 259,910 - IAI47_20135 0.17 +0.6 259,910 - IAI47_20135 0.17 +1.2 259,911 + IAI47_20135 0.17 +2.0 259,911 + IAI47_20135 0.17 +3.4 259,911 + IAI47_20135 0.17 -0.4 259,911 + IAI47_20135 0.17 +1.2 259,911 + IAI47_20135 0.17 -1.6 259,911 + IAI47_20135 0.17 -1.6 259,912 - IAI47_20135 0.17 +1.1 259,912 - IAI47_20135 0.17 +2.1 259,912 - IAI47_20135 0.17 +1.3 259,912 - IAI47_20135 0.17 +2.0 259,912 - IAI47_20135 0.17 +0.3 259,912 - IAI47_20135 0.17 +1.0 259,912 - IAI47_20135 0.17 +0.8 259,912 - IAI47_20135 0.17 -2.3 259,912 - IAI47_20135 0.17 -0.6 259,945 + IAI47_20135 0.25 -2.2 259,946 - IAI47_20135 0.25 +1.2 260,043 + IAI47_20135 0.47 -0.7 260,043 + IAI47_20135 0.47 +3.4 260,043 + IAI47_20135 0.47 -0.4 260,043 + IAI47_20135 0.47 +1.2 260,043 + IAI47_20135 0.47 +1.1 260,043 + IAI47_20135 0.47 -0.4 260,043 + IAI47_20135 0.47 +1.4 260,044 - IAI47_20135 0.47 -0.8 260,044 - IAI47_20135 0.47 +1.0 260,044 - IAI47_20135 0.47 +1.4 260,044 - IAI47_20135 0.47 -0.2 260,057 + IAI47_20135 0.50 -0.5 260,057 + IAI47_20135 0.50 -2.3 260,057 + IAI47_20135 0.50 +0.3 260,057 + IAI47_20135 0.50 +0.8 260,058 - IAI47_20135 0.51 -0.7 260,058 - IAI47_20135 0.51 +0.2 260,058 - IAI47_20135 0.51 +0.7 260,058 - IAI47_20135 0.51 +1.1 260,120 + IAI47_20135 0.65 +2.4 260,166 + IAI47_20135 0.75 +1.0 260,166 + IAI47_20135 0.75 -2.4 260,166 + IAI47_20135 0.75 -0.2 260,167 - IAI47_20135 0.76 +1.5 260,167 - IAI47_20135 0.76 -1.0 260,245 + -0.4 260,245 + -1.6 260,246 - +1.0 260,248 + +0.0 260,248 + -2.3 260,249 - +0.3 260,249 - -2.6
Or see this region's nucleotide sequence