Experiment: Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_06385 and IAI47_06390 overlap by 4 nucleotides IAI47_06390 and IAI47_06395 are separated by 11 nucleotides IAI47_06395 and IAI47_06400 are separated by 10 nucleotides IAI47_06400 and IAI47_06405 overlap by 4 nucleotides IAI47_06405 and IAI47_06410 overlap by 4 nucleotides
IAI47_06385: IAI47_06385 - NADH-quinone oxidoreductase subunit NuoG, at 1,298,869 to 1,301,592
_06385
IAI47_06390: IAI47_06390 - NADH-quinone oxidoreductase subunit NuoH, at 1,301,589 to 1,302,566
_06390
IAI47_06395: IAI47_06395 - NADH-quinone oxidoreductase subunit NuoI, at 1,302,578 to 1,303,120
_06395
IAI47_06400: IAI47_06400 - NADH-quinone oxidoreductase subunit J, at 1,303,131 to 1,303,682
_06400
IAI47_06405: IAI47_06405 - NADH-quinone oxidoreductase subunit NuoK, at 1,303,679 to 1,303,981
_06405
IAI47_06410: IAI47_06410 - NADH-quinone oxidoreductase subunit L, at 1,303,978 to 1,305,813
_06410
Position (kb)
1302
1303
1304 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1301.795 kb on + strand, within IAI47_06390 at 1301.819 kb on + strand, within IAI47_06390 at 1301.820 kb on - strand, within IAI47_06390 at 1301.831 kb on + strand, within IAI47_06390 at 1301.885 kb on + strand, within IAI47_06390 at 1301.970 kb on + strand, within IAI47_06390 at 1302.054 kb on + strand, within IAI47_06390 at 1302.188 kb on + strand at 1302.313 kb on - strand, within IAI47_06390 at 1302.313 kb on - strand, within IAI47_06390 at 1302.422 kb on + strand, within IAI47_06390 at 1302.423 kb on - strand, within IAI47_06390 at 1302.423 kb on - strand, within IAI47_06390 at 1302.592 kb on + strand at 1302.601 kb on - strand at 1302.625 kb on + strand at 1302.626 kb on - strand at 1302.799 kb on + strand, within IAI47_06395 at 1302.971 kb on + strand, within IAI47_06395 at 1302.971 kb on + strand, within IAI47_06395 at 1302.971 kb on + strand, within IAI47_06395 at 1303.043 kb on - strand at 1303.063 kb on + strand, within IAI47_06395 at 1303.093 kb on + strand at 1303.110 kb on + strand at 1303.110 kb on + strand at 1303.110 kb on + strand at 1303.110 kb on + strand at 1303.110 kb on + strand at 1303.113 kb on + strand at 1303.113 kb on + strand at 1303.113 kb on + strand at 1303.118 kb on + strand at 1303.146 kb on + strand at 1303.275 kb on + strand, within IAI47_06400 at 1303.276 kb on - strand, within IAI47_06400 at 1303.343 kb on + strand, within IAI47_06400 at 1303.456 kb on - strand, within IAI47_06400 at 1303.539 kb on + strand, within IAI47_06400 at 1303.539 kb on + strand, within IAI47_06400 at 1303.540 kb on - strand, within IAI47_06400 at 1303.565 kb on + strand, within IAI47_06400 at 1303.566 kb on - strand, within IAI47_06400 at 1303.643 kb on + strand at 1303.689 kb on + strand at 1303.750 kb on - strand, within IAI47_06405 at 1303.809 kb on + strand, within IAI47_06405 at 1303.809 kb on + strand, within IAI47_06405 at 1303.809 kb on + strand, within IAI47_06405 at 1303.832 kb on + strand, within IAI47_06405 at 1303.862 kb on + strand, within IAI47_06405 at 1303.862 kb on + strand, within IAI47_06405 at 1303.862 kb on + strand, within IAI47_06405 at 1303.862 kb on + strand, within IAI47_06405 at 1303.863 kb on - strand, within IAI47_06405 at 1303.863 kb on - strand, within IAI47_06405 at 1303.863 kb on - strand, within IAI47_06405 at 1303.863 kb on - strand, within IAI47_06405 at 1303.863 kb on - strand, within IAI47_06405 at 1303.863 kb on - strand, within IAI47_06405 at 1303.928 kb on + strand, within IAI47_06405 at 1303.957 kb on + strand at 1303.957 kb on + strand at 1303.959 kb on - strand at 1303.995 kb on - strand at 1303.995 kb on - strand at 1304.030 kb on + strand at 1304.075 kb on + strand at 1304.076 kb on - strand at 1304.079 kb on + strand at 1304.079 kb on + strand at 1304.080 kb on - strand at 1304.109 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 1,301,795 + IAI47_06390 0.21 -0.6 1,301,819 + IAI47_06390 0.24 +1.4 1,301,820 - IAI47_06390 0.24 -0.6 1,301,831 + IAI47_06390 0.25 -0.8 1,301,885 + IAI47_06390 0.30 +0.5 1,301,970 + IAI47_06390 0.39 +1.4 1,302,054 + IAI47_06390 0.48 -1.0 1,302,188 + -0.6 1,302,313 - IAI47_06390 0.74 +1.1 1,302,313 - IAI47_06390 0.74 +0.9 1,302,422 + IAI47_06390 0.85 -0.2 1,302,423 - IAI47_06390 0.85 +0.4 1,302,423 - IAI47_06390 0.85 +0.8 1,302,592 + +0.4 1,302,601 - +0.4 1,302,625 + +1.4 1,302,626 - +1.4 1,302,799 + IAI47_06395 0.41 -0.2 1,302,971 + IAI47_06395 0.72 +0.4 1,302,971 + IAI47_06395 0.72 -0.6 1,302,971 + IAI47_06395 0.72 -1.0 1,303,043 - -1.0 1,303,063 + IAI47_06395 0.89 -1.4 1,303,093 + +3.5 1,303,110 + -1.8 1,303,110 + -1.2 1,303,110 + +0.5 1,303,110 + +1.2 1,303,110 + +1.7 1,303,113 + -3.5 1,303,113 + -2.0 1,303,113 + -0.7 1,303,118 + -1.0 1,303,146 + -1.0 1,303,275 + IAI47_06400 0.26 -0.2 1,303,276 - IAI47_06400 0.26 +0.1 1,303,343 + IAI47_06400 0.38 +0.4 1,303,456 - IAI47_06400 0.59 +1.4 1,303,539 + IAI47_06400 0.74 +0.4 1,303,539 + IAI47_06400 0.74 -0.4 1,303,540 - IAI47_06400 0.74 -0.6 1,303,565 + IAI47_06400 0.79 +1.7 1,303,566 - IAI47_06400 0.79 +1.4 1,303,643 + -1.5 1,303,689 + +1.4 1,303,750 - IAI47_06405 0.23 -0.2 1,303,809 + IAI47_06405 0.43 +1.7 1,303,809 + IAI47_06405 0.43 +2.2 1,303,809 + IAI47_06405 0.43 -1.4 1,303,832 + IAI47_06405 0.50 +1.4 1,303,862 + IAI47_06405 0.60 -0.9 1,303,862 + IAI47_06405 0.60 -1.8 1,303,862 + IAI47_06405 0.60 +0.4 1,303,862 + IAI47_06405 0.60 +1.4 1,303,863 - IAI47_06405 0.61 -1.6 1,303,863 - IAI47_06405 0.61 -0.6 1,303,863 - IAI47_06405 0.61 -0.2 1,303,863 - IAI47_06405 0.61 -0.6 1,303,863 - IAI47_06405 0.61 -0.6 1,303,863 - IAI47_06405 0.61 -0.9 1,303,928 + IAI47_06405 0.82 +1.4 1,303,957 + +0.4 1,303,957 + +2.0 1,303,959 - +0.4 1,303,995 - +1.4 1,303,995 - -0.2 1,304,030 + -0.9 1,304,075 + -0.2 1,304,076 - -1.2 1,304,079 + +2.0 1,304,079 + -0.9 1,304,080 - -0.2 1,304,109 + -0.2
Or see this region's nucleotide sequence