Experiment: a-Cyclodextrin (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14135 and BWI76_RS14140 are separated by 2 nucleotides BWI76_RS14140 and BWI76_RS14145 are separated by 43 nucleotides
BWI76_RS14135: BWI76_RS14135 - malto-oligosyltrehalose synthase, at 2,953,943 to 2,956,483
_RS14135
BWI76_RS14140: BWI76_RS14140 - glycogen debranching enzyme, at 2,956,486 to 2,958,561
_RS14140
BWI76_RS14145: BWI76_RS14145 - dihydrolipoyl dehydrogenase, at 2,958,605 to 2,960,002
_RS14145
Position (kb)
2956
2957
2958
2959 Strain fitness (log2 ratio)
-1
0
1 at 2955.573 kb on + strand, within BWI76_RS14135 at 2955.675 kb on + strand, within BWI76_RS14135 at 2955.995 kb on - strand, within BWI76_RS14135 at 2956.080 kb on - strand, within BWI76_RS14135 at 2956.263 kb on - strand at 2956.575 kb on + strand at 2956.595 kb on + strand at 2956.601 kb on + strand at 2957.044 kb on - strand, within BWI76_RS14140 at 2957.300 kb on + strand, within BWI76_RS14140 at 2957.308 kb on - strand, within BWI76_RS14140 at 2957.496 kb on + strand, within BWI76_RS14140 at 2957.702 kb on - strand, within BWI76_RS14140 at 2957.797 kb on - strand, within BWI76_RS14140 at 2957.866 kb on + strand, within BWI76_RS14140 at 2957.966 kb on + strand, within BWI76_RS14140 at 2958.126 kb on - strand, within BWI76_RS14140 at 2958.178 kb on - strand, within BWI76_RS14140 at 2958.296 kb on + strand, within BWI76_RS14140 at 2958.322 kb on + strand, within BWI76_RS14140 at 2958.462 kb on - strand at 2958.700 kb on + strand at 2958.700 kb on + strand at 2958.743 kb on - strand at 2958.830 kb on - strand, within BWI76_RS14145 at 2959.053 kb on + strand, within BWI76_RS14145 at 2959.278 kb on + strand, within BWI76_RS14145
Per-strain Table
Position Strand Gene LocusTag Fraction a-Cyclodextrin (C) remove 2,955,573 + BWI76_RS14135 0.64 -0.4 2,955,675 + BWI76_RS14135 0.68 +0.5 2,955,995 - BWI76_RS14135 0.81 +0.7 2,956,080 - BWI76_RS14135 0.84 +0.5 2,956,263 - +0.3 2,956,575 + +0.1 2,956,595 + +1.3 2,956,601 + +1.1 2,957,044 - BWI76_RS14140 0.27 +0.3 2,957,300 + BWI76_RS14140 0.39 -0.4 2,957,308 - BWI76_RS14140 0.40 -0.1 2,957,496 + BWI76_RS14140 0.49 +0.3 2,957,702 - BWI76_RS14140 0.59 -0.5 2,957,797 - BWI76_RS14140 0.63 +0.4 2,957,866 + BWI76_RS14140 0.66 -0.1 2,957,966 + BWI76_RS14140 0.71 +0.1 2,958,126 - BWI76_RS14140 0.79 +0.5 2,958,178 - BWI76_RS14140 0.82 -0.2 2,958,296 + BWI76_RS14140 0.87 -0.0 2,958,322 + BWI76_RS14140 0.88 -0.5 2,958,462 - +0.2 2,958,700 + -0.1 2,958,700 + -1.0 2,958,743 - +0.4 2,958,830 - BWI76_RS14145 0.16 +0.7 2,959,053 + BWI76_RS14145 0.32 -0.1 2,959,278 + BWI76_RS14145 0.48 +0.0
Or see this region's nucleotide sequence