Experiment: a-Cyclodextrin (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02565 and BWI76_RS02570 are separated by 151 nucleotides BWI76_RS02570 and BWI76_RS02575 are separated by 37 nucleotides BWI76_RS02575 and BWI76_RS02580 are separated by 85 nucleotides BWI76_RS02580 and BWI76_RS02585 are separated by 45 nucleotides
BWI76_RS02565: BWI76_RS02565 - adenylosuccinate synthase, at 524,823 to 526,121
_RS02565
BWI76_RS02570: BWI76_RS02570 - transcriptional regulator, at 526,273 to 526,698
_RS02570
BWI76_RS02575: BWI76_RS02575 - exoribonuclease R, at 526,736 to 529,183
_RS02575
BWI76_RS02580: BWI76_RS02580 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase, at 529,269 to 530,000
_RS02580
BWI76_RS02585: BWI76_RS02585 - hypothetical protein, at 530,046 to 530,399
_RS02585
Position (kb) 526
527
528
529
530 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 526.147 kb on - strand at 526.246 kb on + strand at 526.319 kb on + strand, within BWI76_RS02570 at 526.322 kb on - strand, within BWI76_RS02570 at 526.429 kb on + strand, within BWI76_RS02570 at 526.429 kb on + strand, within BWI76_RS02570 at 526.437 kb on - strand, within BWI76_RS02570 at 526.437 kb on - strand, within BWI76_RS02570 at 526.460 kb on + strand, within BWI76_RS02570 at 526.517 kb on + strand, within BWI76_RS02570 at 526.580 kb on + strand, within BWI76_RS02570 at 526.635 kb on + strand, within BWI76_RS02570 at 526.715 kb on + strand at 526.785 kb on - strand at 526.864 kb on + strand at 526.977 kb on + strand at 526.985 kb on - strand, within BWI76_RS02575 at 526.987 kb on + strand, within BWI76_RS02575 at 527.269 kb on - strand, within BWI76_RS02575 at 527.439 kb on + strand, within BWI76_RS02575 at 527.519 kb on + strand, within BWI76_RS02575 at 527.519 kb on + strand, within BWI76_RS02575 at 527.587 kb on - strand, within BWI76_RS02575 at 527.843 kb on - strand, within BWI76_RS02575 at 527.881 kb on - strand, within BWI76_RS02575 at 528.072 kb on + strand, within BWI76_RS02575 at 528.459 kb on + strand, within BWI76_RS02575 at 528.757 kb on - strand, within BWI76_RS02575 at 528.884 kb on - strand, within BWI76_RS02575 at 529.089 kb on - strand at 529.149 kb on - strand at 529.667 kb on + strand, within BWI76_RS02580 at 529.792 kb on + strand, within BWI76_RS02580 at 529.804 kb on + strand, within BWI76_RS02580 at 529.971 kb on - strand at 530.042 kb on - strand at 530.042 kb on - strand at 530.114 kb on - strand, within BWI76_RS02585 at 530.114 kb on - strand, within BWI76_RS02585
Per-strain Table
Position Strand Gene LocusTag Fraction a-Cyclodextrin (C) remove 526,147 - +0.2 526,246 + +0.2 526,319 + BWI76_RS02570 0.11 -0.2 526,322 - BWI76_RS02570 0.12 +0.9 526,429 + BWI76_RS02570 0.37 +0.5 526,429 + BWI76_RS02570 0.37 +0.7 526,437 - BWI76_RS02570 0.38 +0.1 526,437 - BWI76_RS02570 0.38 +0.9 526,460 + BWI76_RS02570 0.44 +0.1 526,517 + BWI76_RS02570 0.57 +0.2 526,580 + BWI76_RS02570 0.72 -0.0 526,635 + BWI76_RS02570 0.85 -1.1 526,715 + -0.7 526,785 - -2.3 526,864 + -0.7 526,977 + -2.9 526,985 - BWI76_RS02575 0.10 -1.0 526,987 + BWI76_RS02575 0.10 -1.4 527,269 - BWI76_RS02575 0.22 -0.3 527,439 + BWI76_RS02575 0.29 +0.2 527,519 + BWI76_RS02575 0.32 +0.1 527,519 + BWI76_RS02575 0.32 -2.1 527,587 - BWI76_RS02575 0.35 -0.7 527,843 - BWI76_RS02575 0.45 -0.8 527,881 - BWI76_RS02575 0.47 -2.0 528,072 + BWI76_RS02575 0.55 -0.9 528,459 + BWI76_RS02575 0.70 -0.9 528,757 - BWI76_RS02575 0.83 -2.3 528,884 - BWI76_RS02575 0.88 -1.2 529,089 - -1.3 529,149 - -0.1 529,667 + BWI76_RS02580 0.54 -1.9 529,792 + BWI76_RS02580 0.71 +0.7 529,804 + BWI76_RS02580 0.73 -0.1 529,971 - -0.2 530,042 - +0.7 530,042 - -0.6 530,114 - BWI76_RS02585 0.19 -0.3 530,114 - BWI76_RS02585 0.19 +0.3
Or see this region's nucleotide sequence