Experiment: 4-Hydroxyacetophenone 0.00025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_827 and Pf6N2E2_828 are separated by 127 nucleotides Pf6N2E2_828 and Pf6N2E2_829 are separated by 201 nucleotides
Pf6N2E2_827 - probable dioxygenase, at 965,843 to 966,268
_827
Pf6N2E2_828 - Transcriptional regulator, TetR family, at 966,396 to 967,016
_828
Pf6N2E2_829 - TesB-like acyl-CoA thioesterase 1, at 967,218 to 967,997
_829
Position (kb)
966
967
968 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 965.463 kb on + strand at 965.542 kb on + strand at 965.542 kb on + strand at 965.543 kb on - strand at 965.543 kb on - strand at 965.543 kb on - strand at 965.543 kb on - strand at 965.660 kb on + strand at 965.660 kb on + strand at 965.660 kb on + strand at 965.660 kb on + strand at 965.661 kb on - strand at 965.661 kb on - strand at 965.662 kb on + strand at 965.662 kb on + strand at 965.663 kb on - strand at 965.663 kb on - strand at 965.663 kb on - strand at 965.663 kb on - strand at 965.701 kb on + strand at 965.701 kb on + strand at 965.701 kb on + strand at 965.702 kb on - strand at 965.830 kb on - strand at 965.879 kb on + strand at 965.907 kb on - strand, within Pf6N2E2_827 at 965.907 kb on - strand, within Pf6N2E2_827 at 965.970 kb on - strand, within Pf6N2E2_827 at 966.152 kb on + strand, within Pf6N2E2_827 at 966.384 kb on + strand at 966.384 kb on + strand at 966.496 kb on - strand, within Pf6N2E2_828 at 966.555 kb on + strand, within Pf6N2E2_828 at 966.555 kb on + strand, within Pf6N2E2_828 at 966.556 kb on - strand, within Pf6N2E2_828 at 966.556 kb on - strand, within Pf6N2E2_828 at 966.556 kb on - strand, within Pf6N2E2_828 at 966.561 kb on + strand, within Pf6N2E2_828 at 966.562 kb on - strand, within Pf6N2E2_828 at 966.747 kb on + strand, within Pf6N2E2_828 at 966.747 kb on + strand, within Pf6N2E2_828 at 966.799 kb on - strand, within Pf6N2E2_828 at 966.934 kb on + strand, within Pf6N2E2_828 at 966.945 kb on + strand, within Pf6N2E2_828 at 966.945 kb on + strand, within Pf6N2E2_828 at 967.077 kb on - strand at 967.142 kb on + strand at 967.215 kb on + strand at 967.216 kb on - strand at 967.216 kb on - strand at 967.216 kb on - strand at 967.399 kb on + strand, within Pf6N2E2_829 at 967.399 kb on + strand, within Pf6N2E2_829 at 967.399 kb on + strand, within Pf6N2E2_829 at 967.400 kb on - strand, within Pf6N2E2_829 at 967.400 kb on - strand, within Pf6N2E2_829 at 967.928 kb on + strand at 967.991 kb on + strand at 967.992 kb on - strand at 967.992 kb on - strand at 967.995 kb on + strand at 967.996 kb on - strand at 967.998 kb on + strand at 967.998 kb on + strand at 967.999 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxyacetophenone 0.00025 M remove 965,463 + -1.4 965,542 + +0.5 965,542 + +1.4 965,543 - +0.1 965,543 - -0.8 965,543 - -0.7 965,543 - -0.1 965,660 + -0.7 965,660 + -0.1 965,660 + +0.5 965,660 + +1.5 965,661 - -0.5 965,661 - -1.1 965,662 + +0.5 965,662 + +0.1 965,663 - +0.7 965,663 - +0.0 965,663 - +1.5 965,663 - -1.8 965,701 + +1.2 965,701 + +0.2 965,701 + -0.6 965,702 - +0.9 965,830 - -0.4 965,879 + +0.3 965,907 - Pf6N2E2_827 0.15 -0.6 965,907 - Pf6N2E2_827 0.15 -0.8 965,970 - Pf6N2E2_827 0.30 +0.7 966,152 + Pf6N2E2_827 0.73 -0.8 966,384 + +0.8 966,384 + +0.4 966,496 - Pf6N2E2_828 0.16 -2.4 966,555 + Pf6N2E2_828 0.26 -0.7 966,555 + Pf6N2E2_828 0.26 -3.0 966,556 - Pf6N2E2_828 0.26 -0.5 966,556 - Pf6N2E2_828 0.26 +0.2 966,556 - Pf6N2E2_828 0.26 -1.1 966,561 + Pf6N2E2_828 0.27 -2.1 966,562 - Pf6N2E2_828 0.27 -2.0 966,747 + Pf6N2E2_828 0.57 -1.1 966,747 + Pf6N2E2_828 0.57 +0.2 966,799 - Pf6N2E2_828 0.65 -1.7 966,934 + Pf6N2E2_828 0.87 -0.8 966,945 + Pf6N2E2_828 0.88 -1.5 966,945 + Pf6N2E2_828 0.88 +0.3 967,077 - +0.2 967,142 + -1.0 967,215 + +0.6 967,216 - +0.8 967,216 - -0.1 967,216 - -1.1 967,399 + Pf6N2E2_829 0.23 -0.3 967,399 + Pf6N2E2_829 0.23 -0.3 967,399 + Pf6N2E2_829 0.23 +0.7 967,400 - Pf6N2E2_829 0.23 -0.8 967,400 - Pf6N2E2_829 0.23 +1.1 967,928 + -0.8 967,991 + -1.0 967,992 - +0.3 967,992 - +1.1 967,995 + -1.5 967,996 - +0.5 967,998 + -0.5 967,998 + -0.3 967,999 - +0.1
Or see this region's nucleotide sequence