Experiment: 4-Hydroxyacetophenone 0.00025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5475 and Pf6N2E2_5476 overlap by 4 nucleotides Pf6N2E2_5476 and Pf6N2E2_5477 are separated by 215 nucleotides
Pf6N2E2_5475 - Acetolactate synthase, large subunit (EC 2.2.1.6), at 6,291,769 to 6,293,406
_5475
Pf6N2E2_5476 - Aldehyde dehydrogenase (EC 1.2.1.3), at 6,293,403 to 6,294,851
_5476
Pf6N2E2_5477 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 6,295,067 to 6,296,170
_5477
Position (kb)
6293
6294
6295 Strain fitness (log2 ratio)
-1
0
1
2 at 6292.603 kb on + strand, within Pf6N2E2_5475 at 6292.688 kb on - strand, within Pf6N2E2_5475 at 6292.930 kb on + strand, within Pf6N2E2_5475 at 6292.931 kb on - strand, within Pf6N2E2_5475 at 6292.931 kb on - strand, within Pf6N2E2_5475 at 6293.172 kb on + strand, within Pf6N2E2_5475 at 6293.183 kb on - strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.200 kb on + strand, within Pf6N2E2_5475 at 6293.201 kb on - strand, within Pf6N2E2_5475 at 6293.201 kb on - strand, within Pf6N2E2_5475 at 6293.201 kb on - strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.242 kb on + strand, within Pf6N2E2_5475 at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.243 kb on - strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.433 kb on + strand at 6293.434 kb on - strand at 6293.434 kb on - strand at 6293.434 kb on - strand at 6293.434 kb on - strand at 6293.434 kb on - strand at 6293.739 kb on + strand, within Pf6N2E2_5476 at 6293.740 kb on - strand, within Pf6N2E2_5476 at 6293.742 kb on + strand, within Pf6N2E2_5476 at 6294.406 kb on - strand, within Pf6N2E2_5476 at 6294.765 kb on + strand at 6294.766 kb on - strand at 6294.766 kb on - strand at 6295.041 kb on + strand at 6295.042 kb on - strand at 6295.042 kb on - strand at 6295.042 kb on - strand at 6295.045 kb on + strand at 6295.045 kb on + strand at 6295.045 kb on + strand at 6295.046 kb on - strand at 6295.053 kb on + strand at 6295.054 kb on - strand at 6295.306 kb on + strand, within Pf6N2E2_5477 at 6295.356 kb on - strand, within Pf6N2E2_5477 at 6295.356 kb on - strand, within Pf6N2E2_5477 at 6295.363 kb on + strand, within Pf6N2E2_5477 at 6295.363 kb on + strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.499 kb on + strand, within Pf6N2E2_5477 at 6295.526 kb on + strand, within Pf6N2E2_5477 at 6295.537 kb on + strand, within Pf6N2E2_5477 at 6295.562 kb on + strand, within Pf6N2E2_5477 at 6295.562 kb on + strand, within Pf6N2E2_5477 at 6295.563 kb on - strand, within Pf6N2E2_5477 at 6295.799 kb on + strand, within Pf6N2E2_5477 at 6295.800 kb on - strand, within Pf6N2E2_5477 at 6295.800 kb on - strand, within Pf6N2E2_5477 at 6295.841 kb on + strand, within Pf6N2E2_5477 at 6295.842 kb on - strand, within Pf6N2E2_5477
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxyacetophenone 0.00025 M remove 6,292,603 + Pf6N2E2_5475 0.51 -0.2 6,292,688 - Pf6N2E2_5475 0.56 -0.9 6,292,930 + Pf6N2E2_5475 0.71 -0.4 6,292,931 - Pf6N2E2_5475 0.71 -1.3 6,292,931 - Pf6N2E2_5475 0.71 -0.8 6,293,172 + Pf6N2E2_5475 0.86 +1.1 6,293,183 - Pf6N2E2_5475 0.86 -0.3 6,293,200 + Pf6N2E2_5475 0.87 +1.0 6,293,200 + Pf6N2E2_5475 0.87 -0.9 6,293,200 + Pf6N2E2_5475 0.87 +1.7 6,293,200 + Pf6N2E2_5475 0.87 +0.6 6,293,200 + Pf6N2E2_5475 0.87 +1.0 6,293,200 + Pf6N2E2_5475 0.87 +0.0 6,293,201 - Pf6N2E2_5475 0.87 -1.0 6,293,201 - Pf6N2E2_5475 0.87 +0.3 6,293,201 - Pf6N2E2_5475 0.87 +0.4 6,293,242 + Pf6N2E2_5475 0.90 -1.4 6,293,242 + Pf6N2E2_5475 0.90 -0.3 6,293,242 + Pf6N2E2_5475 0.90 +1.1 6,293,242 + Pf6N2E2_5475 0.90 +0.5 6,293,242 + Pf6N2E2_5475 0.90 +0.9 6,293,242 + Pf6N2E2_5475 0.90 +0.7 6,293,242 + Pf6N2E2_5475 0.90 +0.2 6,293,242 + Pf6N2E2_5475 0.90 +0.8 6,293,242 + Pf6N2E2_5475 0.90 +0.0 6,293,242 + Pf6N2E2_5475 0.90 +0.3 6,293,243 - +2.1 6,293,243 - +0.5 6,293,243 - +0.7 6,293,243 - -0.7 6,293,243 - -0.9 6,293,243 - -1.1 6,293,243 - -0.8 6,293,243 - +1.8 6,293,433 + -1.3 6,293,433 + -1.2 6,293,433 + -1.1 6,293,433 + +0.5 6,293,433 + -0.7 6,293,433 + +0.2 6,293,433 + -0.1 6,293,433 + +0.3 6,293,434 - -0.8 6,293,434 - +1.3 6,293,434 - +0.4 6,293,434 - +0.1 6,293,434 - -1.0 6,293,739 + Pf6N2E2_5476 0.23 -1.6 6,293,740 - Pf6N2E2_5476 0.23 -0.8 6,293,742 + Pf6N2E2_5476 0.23 -0.2 6,294,406 - Pf6N2E2_5476 0.69 -0.5 6,294,765 + -0.7 6,294,766 - +0.9 6,294,766 - +0.2 6,295,041 + -1.3 6,295,042 - +0.3 6,295,042 - +0.6 6,295,042 - +0.3 6,295,045 + +0.3 6,295,045 + +0.2 6,295,045 + +0.5 6,295,046 - +0.1 6,295,053 + +0.2 6,295,054 - +0.7 6,295,306 + Pf6N2E2_5477 0.22 -0.1 6,295,356 - Pf6N2E2_5477 0.26 -1.1 6,295,356 - Pf6N2E2_5477 0.26 +0.6 6,295,363 + Pf6N2E2_5477 0.27 +0.6 6,295,363 + Pf6N2E2_5477 0.27 +0.7 6,295,364 - Pf6N2E2_5477 0.27 -0.5 6,295,364 - Pf6N2E2_5477 0.27 +0.4 6,295,364 - Pf6N2E2_5477 0.27 -0.1 6,295,499 + Pf6N2E2_5477 0.39 -0.1 6,295,526 + Pf6N2E2_5477 0.42 +0.6 6,295,537 + Pf6N2E2_5477 0.43 -0.2 6,295,562 + Pf6N2E2_5477 0.45 +0.3 6,295,562 + Pf6N2E2_5477 0.45 +0.5 6,295,563 - Pf6N2E2_5477 0.45 -0.2 6,295,799 + Pf6N2E2_5477 0.66 +0.7 6,295,800 - Pf6N2E2_5477 0.66 +0.5 6,295,800 - Pf6N2E2_5477 0.66 +0.4 6,295,841 + Pf6N2E2_5477 0.70 -0.8 6,295,842 - Pf6N2E2_5477 0.70 +0.6
Or see this region's nucleotide sequence