Experiment: 4-Hydroxyacetophenone 0.00025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1438 and Pf6N2E2_1439 are separated by 237 nucleotides Pf6N2E2_1439 and Pf6N2E2_1440 are separated by 469 nucleotides
Pf6N2E2_1438 - Methyltransferase (EC 2.1.1.-), at 1,688,548 to 1,689,132
_1438
Pf6N2E2_1439 - hypothetical protein, at 1,689,370 to 1,689,948
_1439
Pf6N2E2_1440 - Putative tRNA-m1A22 methylase, at 1,690,418 to 1,691,113
_1440
Position (kb)
1689
1690 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1688.391 kb on - strand at 1688.391 kb on - strand at 1688.391 kb on - strand at 1688.396 kb on - strand at 1688.416 kb on + strand at 1688.416 kb on + strand at 1688.417 kb on - strand at 1688.417 kb on - strand at 1688.417 kb on - strand at 1688.450 kb on + strand at 1688.507 kb on + strand at 1688.508 kb on - strand at 1688.744 kb on + strand, within Pf6N2E2_1438 at 1688.745 kb on - strand, within Pf6N2E2_1438 at 1688.821 kb on - strand, within Pf6N2E2_1438 at 1688.821 kb on - strand, within Pf6N2E2_1438 at 1688.930 kb on - strand, within Pf6N2E2_1438 at 1688.988 kb on + strand, within Pf6N2E2_1438 at 1688.989 kb on - strand, within Pf6N2E2_1438 at 1689.095 kb on - strand at 1689.109 kb on + strand at 1689.110 kb on - strand at 1689.234 kb on - strand at 1689.234 kb on - strand at 1689.324 kb on - strand at 1689.379 kb on + strand at 1689.380 kb on - strand at 1689.445 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.481 kb on + strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.482 kb on - strand, within Pf6N2E2_1439 at 1689.672 kb on - strand, within Pf6N2E2_1439 at 1689.672 kb on - strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.685 kb on + strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.686 kb on - strand, within Pf6N2E2_1439 at 1689.706 kb on + strand, within Pf6N2E2_1439 at 1689.707 kb on - strand, within Pf6N2E2_1439 at 1689.781 kb on + strand, within Pf6N2E2_1439 at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.094 kb on - strand at 1690.098 kb on - strand at 1690.098 kb on - strand at 1690.098 kb on - strand at 1690.227 kb on + strand at 1690.228 kb on - strand at 1690.277 kb on - strand at 1690.277 kb on - strand at 1690.330 kb on - strand at 1690.330 kb on - strand at 1690.340 kb on + strand at 1690.351 kb on - strand at 1690.596 kb on + strand, within Pf6N2E2_1440 at 1690.596 kb on + strand, within Pf6N2E2_1440 at 1690.614 kb on + strand, within Pf6N2E2_1440 at 1690.615 kb on - strand, within Pf6N2E2_1440 at 1690.751 kb on + strand, within Pf6N2E2_1440 at 1690.751 kb on + strand, within Pf6N2E2_1440 at 1690.752 kb on - strand, within Pf6N2E2_1440 at 1690.752 kb on - strand, within Pf6N2E2_1440
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxyacetophenone 0.00025 M remove 1,688,391 - +0.4 1,688,391 - +0.5 1,688,391 - -1.1 1,688,396 - +0.7 1,688,416 + +0.2 1,688,416 + +0.9 1,688,417 - +2.3 1,688,417 - +0.1 1,688,417 - -0.5 1,688,450 + +2.0 1,688,507 + +0.1 1,688,508 - -0.6 1,688,744 + Pf6N2E2_1438 0.34 -0.1 1,688,745 - Pf6N2E2_1438 0.34 +0.0 1,688,821 - Pf6N2E2_1438 0.47 -0.6 1,688,821 - Pf6N2E2_1438 0.47 +0.5 1,688,930 - Pf6N2E2_1438 0.65 +0.7 1,688,988 + Pf6N2E2_1438 0.75 +0.3 1,688,989 - Pf6N2E2_1438 0.75 -0.3 1,689,095 - +0.8 1,689,109 + +1.0 1,689,110 - +0.1 1,689,234 - +0.4 1,689,234 - +0.0 1,689,324 - +0.8 1,689,379 + +2.0 1,689,380 - -0.6 1,689,445 + Pf6N2E2_1439 0.13 -0.3 1,689,481 + Pf6N2E2_1439 0.19 +0.7 1,689,481 + Pf6N2E2_1439 0.19 +0.4 1,689,481 + Pf6N2E2_1439 0.19 +3.0 1,689,481 + Pf6N2E2_1439 0.19 +0.6 1,689,482 - Pf6N2E2_1439 0.19 -0.3 1,689,482 - Pf6N2E2_1439 0.19 +1.9 1,689,482 - Pf6N2E2_1439 0.19 +1.4 1,689,672 - Pf6N2E2_1439 0.52 -0.3 1,689,672 - Pf6N2E2_1439 0.52 +0.4 1,689,685 + Pf6N2E2_1439 0.54 +1.4 1,689,685 + Pf6N2E2_1439 0.54 +1.4 1,689,685 + Pf6N2E2_1439 0.54 -0.6 1,689,685 + Pf6N2E2_1439 0.54 +0.8 1,689,685 + Pf6N2E2_1439 0.54 -0.0 1,689,686 - Pf6N2E2_1439 0.55 +0.2 1,689,686 - Pf6N2E2_1439 0.55 +2.4 1,689,686 - Pf6N2E2_1439 0.55 -0.2 1,689,686 - Pf6N2E2_1439 0.55 +2.0 1,689,706 + Pf6N2E2_1439 0.58 +0.8 1,689,707 - Pf6N2E2_1439 0.58 +1.0 1,689,781 + Pf6N2E2_1439 0.71 -0.2 1,690,094 - -0.5 1,690,094 - -0.9 1,690,094 - +0.5 1,690,094 - -0.0 1,690,094 - -0.2 1,690,094 - +0.4 1,690,098 - -0.4 1,690,098 - +0.3 1,690,098 - -0.1 1,690,227 + +0.8 1,690,228 - -1.1 1,690,277 - -0.5 1,690,277 - -0.5 1,690,330 - +0.1 1,690,330 - -0.5 1,690,340 + +0.9 1,690,351 - +1.1 1,690,596 + Pf6N2E2_1440 0.26 +0.4 1,690,596 + Pf6N2E2_1440 0.26 +0.6 1,690,614 + Pf6N2E2_1440 0.28 +0.4 1,690,615 - Pf6N2E2_1440 0.28 +0.4 1,690,751 + Pf6N2E2_1440 0.48 -2.5 1,690,751 + Pf6N2E2_1440 0.48 -0.2 1,690,752 - Pf6N2E2_1440 0.48 -0.2 1,690,752 - Pf6N2E2_1440 0.48 -0.5
Or see this region's nucleotide sequence