Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_887 and Pf1N1B4_888 are separated by 274 nucleotides Pf1N1B4_888 and Pf1N1B4_889 are separated by 129 nucleotides Pf1N1B4_889 and Pf1N1B4_890 are separated by 98 nucleotides
Pf1N1B4_887 - Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3), at 1,010,235 to 1,014,131
_887
Pf1N1B4_888 - Transglycosylase, Slt family, at 1,014,406 to 1,015,866
_888
Pf1N1B4_889 - Pyridoxine 5'-phosphate synthase (EC 2.6.99.2), at 1,015,996 to 1,016,742
_889
Pf1N1B4_890 - DNA recombination and repair protein RecO, at 1,016,841 to 1,017,530
_890
Position (kb)
1014
1015
1016 Strain fitness (log2 ratio)
-2
-1
0
1 at 1013.885 kb on + strand at 1014.034 kb on + strand at 1014.178 kb on + strand at 1014.285 kb on - strand at 1014.335 kb on + strand at 1014.335 kb on + strand at 1014.336 kb on - strand at 1014.336 kb on - strand at 1014.336 kb on - strand at 1014.372 kb on - strand at 1014.394 kb on + strand at 1014.394 kb on + strand at 1014.395 kb on - strand at 1014.395 kb on - strand at 1014.396 kb on + strand at 1014.396 kb on + strand at 1014.566 kb on - strand, within Pf1N1B4_888 at 1015.036 kb on + strand at 1015.036 kb on + strand, within Pf1N1B4_888 at 1015.037 kb on - strand, within Pf1N1B4_888 at 1015.037 kb on - strand at 1015.141 kb on + strand, within Pf1N1B4_888 at 1015.141 kb on + strand, within Pf1N1B4_888 at 1015.195 kb on + strand at 1015.211 kb on + strand, within Pf1N1B4_888 at 1015.231 kb on + strand, within Pf1N1B4_888 at 1015.232 kb on - strand, within Pf1N1B4_888 at 1015.270 kb on + strand at 1015.271 kb on - strand, within Pf1N1B4_888 at 1015.489 kb on + strand, within Pf1N1B4_888 at 1015.489 kb on + strand, within Pf1N1B4_888 at 1015.489 kb on + strand, within Pf1N1B4_888 at 1015.489 kb on + strand, within Pf1N1B4_888 at 1015.490 kb on - strand, within Pf1N1B4_888 at 1015.533 kb on + strand at 1015.533 kb on + strand, within Pf1N1B4_888 at 1015.675 kb on + strand, within Pf1N1B4_888 at 1015.744 kb on + strand at 1015.747 kb on + strand at 1015.864 kb on + strand at 1015.865 kb on - strand at 1015.865 kb on - strand at 1015.865 kb on - strand at 1016.760 kb on - strand at 1016.783 kb on + strand at 1016.783 kb on + strand at 1016.783 kb on + strand at 1016.783 kb on + strand at 1016.784 kb on - strand at 1016.784 kb on - strand at 1016.784 kb on - strand at 1016.784 kb on - strand at 1016.784 kb on - strand at 1016.828 kb on - strand at 1016.843 kb on - strand at 1016.849 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 1,013,885 + -0.6 1,014,034 + +0.9 1,014,178 + +0.5 1,014,285 - +0.4 1,014,335 + -0.9 1,014,335 + -0.6 1,014,336 - +0.4 1,014,336 - -0.5 1,014,336 - +1.4 1,014,372 - +0.6 1,014,394 + -1.6 1,014,394 + -0.1 1,014,395 - -0.1 1,014,395 - +0.6 1,014,396 + +0.1 1,014,396 + +0.3 1,014,566 - Pf1N1B4_888 0.11 -1.3 1,015,036 + +0.7 1,015,036 + Pf1N1B4_888 0.43 -0.7 1,015,037 - Pf1N1B4_888 0.43 -0.8 1,015,037 - -0.2 1,015,141 + Pf1N1B4_888 0.50 -0.6 1,015,141 + Pf1N1B4_888 0.50 -0.8 1,015,195 + -0.2 1,015,211 + Pf1N1B4_888 0.55 -2.6 1,015,231 + Pf1N1B4_888 0.56 -1.9 1,015,232 - Pf1N1B4_888 0.57 +0.5 1,015,270 + -0.6 1,015,271 - Pf1N1B4_888 0.59 -2.4 1,015,489 + Pf1N1B4_888 0.74 -0.9 1,015,489 + Pf1N1B4_888 0.74 -0.2 1,015,489 + Pf1N1B4_888 0.74 +0.2 1,015,489 + Pf1N1B4_888 0.74 -1.3 1,015,490 - Pf1N1B4_888 0.74 -2.4 1,015,533 + -0.3 1,015,533 + Pf1N1B4_888 0.77 +0.8 1,015,675 + Pf1N1B4_888 0.87 -0.0 1,015,744 + -0.7 1,015,747 + +0.6 1,015,864 + +0.3 1,015,865 - -0.2 1,015,865 - +0.3 1,015,865 - -0.2 1,016,760 - +0.4 1,016,783 + +0.3 1,016,783 + -0.1 1,016,783 + +0.4 1,016,783 + -0.4 1,016,784 - +0.5 1,016,784 - -0.2 1,016,784 - -0.2 1,016,784 - +0.5 1,016,784 - -0.0 1,016,828 - +1.1 1,016,843 - -0.8 1,016,849 + -0.5
Or see this region's nucleotide sequence