Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_865 and Pf1N1B4_866 are separated by 50 nucleotides Pf1N1B4_866 and Pf1N1B4_867 are separated by 295 nucleotides Pf1N1B4_867 and Pf1N1B4_868 are separated by 2 nucleotides Pf1N1B4_868 and Pf1N1B4_869 are separated by 146 nucleotides
Pf1N1B4_865 - Cysteine desulfurase (EC 2.8.1.7), IscS subfamily, at 992,119 to 993,333
_865
Pf1N1B4_866 - Iron-sulfur cluster regulator IscR, at 993,384 to 993,845
_866
Pf1N1B4_867 - Serine acetyltransferase (EC 2.3.1.30), at 994,141 to 994,917
_867
Pf1N1B4_868 - tRNA:Cm32/Um32 methyltransferase, at 994,920 to 995,690
_868
Pf1N1B4_869 - Inositol-1-monophosphatase (EC 3.1.3.25), at 995,837 to 996,655
_869
Position (kb) 994
995 Strain fitness (log2 ratio) -2
-1
0
1
2 at 993.346 kb on - strand at 993.346 kb on - strand at 993.346 kb on - strand at 993.487 kb on - strand, within Pf1N1B4_866 at 993.487 kb on - strand, within Pf1N1B4_866 at 993.487 kb on - strand, within Pf1N1B4_866 at 993.487 kb on - strand, within Pf1N1B4_866 at 993.884 kb on - strand at 993.884 kb on - strand at 993.884 kb on - strand at 993.884 kb on - strand at 993.990 kb on - strand at 994.007 kb on + strand at 994.007 kb on + strand at 994.007 kb on + strand at 994.142 kb on + strand at 994.171 kb on + strand at 994.172 kb on - strand at 994.220 kb on + strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.221 kb on - strand, within Pf1N1B4_867 at 994.273 kb on + strand, within Pf1N1B4_867 at 994.936 kb on - strand at 994.936 kb on - strand at 994.986 kb on + strand at 994.986 kb on + strand at 994.986 kb on + strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.987 kb on - strand at 994.988 kb on + strand at 994.988 kb on + strand at 994.989 kb on - strand at 995.024 kb on - strand, within Pf1N1B4_868 at 995.110 kb on + strand, within Pf1N1B4_868 at 995.111 kb on - strand at 995.111 kb on - strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.216 kb on + strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.217 kb on - strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.227 kb on + strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.228 kb on - strand, within Pf1N1B4_868 at 995.299 kb on + strand, within Pf1N1B4_868 at 995.300 kb on - strand, within Pf1N1B4_868 at 995.388 kb on + strand, within Pf1N1B4_868 at 995.389 kb on - strand, within Pf1N1B4_868 at 995.730 kb on + strand at 995.742 kb on + strand at 995.742 kb on + strand at 995.753 kb on + strand at 995.753 kb on + strand at 995.753 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 993,346 - -0.1 993,346 - -1.3 993,346 - +0.3 993,487 - Pf1N1B4_866 0.22 -0.4 993,487 - Pf1N1B4_866 0.22 +0.8 993,487 - Pf1N1B4_866 0.22 -0.1 993,487 - Pf1N1B4_866 0.22 -0.7 993,884 - +1.6 993,884 - +2.0 993,884 - +1.5 993,884 - -0.3 993,990 - -0.1 994,007 + +1.0 994,007 + -0.3 994,007 + +0.2 994,142 + -0.5 994,171 + +0.2 994,172 - +0.1 994,220 + Pf1N1B4_867 0.10 -2.3 994,221 - Pf1N1B4_867 0.10 -0.3 994,221 - Pf1N1B4_867 0.10 -0.0 994,221 - Pf1N1B4_867 0.10 -0.8 994,221 - Pf1N1B4_867 0.10 +0.2 994,273 + Pf1N1B4_867 0.17 -1.4 994,936 - -0.7 994,936 - +0.4 994,986 + -0.6 994,986 + +1.2 994,986 + -2.1 994,987 - +0.1 994,987 - -1.3 994,987 - +1.2 994,987 - -0.8 994,987 - +0.9 994,987 - -0.2 994,987 - +0.4 994,987 - -0.6 994,987 - -0.5 994,987 - +0.4 994,987 - -0.2 994,987 - +0.3 994,987 - +1.0 994,988 + -1.1 994,988 + +0.7 994,989 - -0.3 995,024 - Pf1N1B4_868 0.13 +1.1 995,110 + Pf1N1B4_868 0.25 -0.1 995,111 - +0.8 995,111 - Pf1N1B4_868 0.25 +0.3 995,216 + Pf1N1B4_868 0.38 -0.3 995,216 + Pf1N1B4_868 0.38 -1.3 995,216 + Pf1N1B4_868 0.38 -1.0 995,216 + Pf1N1B4_868 0.38 -0.5 995,217 - Pf1N1B4_868 0.39 +0.2 995,217 - Pf1N1B4_868 0.39 -0.6 995,217 - Pf1N1B4_868 0.39 +1.4 995,217 - Pf1N1B4_868 0.39 -0.8 995,217 - Pf1N1B4_868 0.39 -1.7 995,217 - Pf1N1B4_868 0.39 +0.0 995,217 - Pf1N1B4_868 0.39 -0.2 995,227 + Pf1N1B4_868 0.40 +1.1 995,227 + Pf1N1B4_868 0.40 -0.3 995,227 + Pf1N1B4_868 0.40 -0.3 995,227 + Pf1N1B4_868 0.40 -0.6 995,227 + Pf1N1B4_868 0.40 -0.7 995,227 + Pf1N1B4_868 0.40 +0.9 995,228 - Pf1N1B4_868 0.40 -0.5 995,228 - Pf1N1B4_868 0.40 -0.0 995,228 - Pf1N1B4_868 0.40 -0.1 995,228 - Pf1N1B4_868 0.40 -0.4 995,228 - Pf1N1B4_868 0.40 -0.6 995,299 + Pf1N1B4_868 0.49 -0.8 995,300 - Pf1N1B4_868 0.49 -0.1 995,388 + Pf1N1B4_868 0.61 -1.6 995,389 - Pf1N1B4_868 0.61 +0.2 995,730 + +0.7 995,742 + -0.9 995,742 + -1.3 995,753 + +0.6 995,753 + -0.3 995,753 + -1.6
Or see this region's nucleotide sequence