Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5419 and Pf1N1B4_5420 are separated by 264 nucleotides Pf1N1B4_5420 and Pf1N1B4_5421 are separated by 112 nucleotides Pf1N1B4_5421 and Pf1N1B4_5422 are separated by 121 nucleotides
Pf1N1B4_5419 - hypothetical protein, at 159,566 to 159,685
_5419
Pf1N1B4_5420 - hypothetical protein, at 159,950 to 160,297
_5420
Pf1N1B4_5421 - hypothetical protein, at 160,410 to 160,661
_5421
Pf1N1B4_5422 - hypothetical protein, at 160,783 to 162,591
_5422
Position (kb) 159
160
161 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 158.982 kb on + strand at 159.039 kb on + strand at 159.046 kb on - strand at 159.136 kb on + strand at 159.165 kb on - strand at 159.359 kb on + strand at 159.465 kb on + strand at 159.497 kb on + strand at 159.497 kb on + strand at 159.497 kb on + strand at 159.497 kb on + strand at 159.567 kb on + strand at 159.685 kb on + strand at 159.685 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.786 kb on - strand at 159.786 kb on - strand at 159.786 kb on - strand at 159.786 kb on - strand at 159.877 kb on + strand at 159.877 kb on + strand at 159.978 kb on + strand at 159.978 kb on + strand at 159.978 kb on + strand at 160.191 kb on + strand, within Pf1N1B4_5420 at 160.192 kb on - strand, within Pf1N1B4_5420 at 160.218 kb on + strand, within Pf1N1B4_5420 at 160.218 kb on + strand, within Pf1N1B4_5420 at 160.219 kb on - strand, within Pf1N1B4_5420 at 160.219 kb on - strand, within Pf1N1B4_5420 at 160.314 kb on + strand at 160.315 kb on - strand at 160.317 kb on + strand at 160.317 kb on + strand at 160.317 kb on + strand at 160.318 kb on - strand at 160.352 kb on - strand at 160.352 kb on - strand at 160.370 kb on - strand at 160.373 kb on + strand at 160.373 kb on + strand at 160.373 kb on + strand at 160.532 kb on - strand, within Pf1N1B4_5421 at 160.636 kb on + strand at 160.636 kb on + strand at 160.636 kb on + strand at 160.636 kb on + strand at 160.637 kb on - strand at 160.693 kb on + strand at 160.694 kb on - strand at 160.695 kb on + strand at 160.695 kb on + strand at 160.695 kb on + strand at 160.695 kb on + strand at 160.695 kb on + strand at 160.696 kb on - strand at 160.696 kb on - strand at 160.696 kb on - strand at 160.696 kb on - strand at 160.696 kb on - strand at 160.747 kb on + strand at 160.747 kb on + strand at 160.748 kb on - strand at 160.748 kb on - strand at 160.748 kb on - strand at 160.748 kb on - strand at 160.748 kb on - strand at 160.749 kb on + strand at 160.749 kb on + strand at 160.749 kb on + strand at 160.749 kb on + strand at 160.749 kb on + strand at 160.749 kb on + strand at 160.750 kb on - strand at 160.750 kb on - strand at 160.750 kb on - strand at 160.750 kb on - strand at 160.750 kb on - strand at 160.750 kb on - strand at 160.774 kb on + strand at 160.774 kb on + strand at 160.804 kb on + strand at 160.823 kb on + strand at 160.851 kb on + strand at 161.100 kb on + strand, within Pf1N1B4_5422 at 161.132 kb on + strand, within Pf1N1B4_5422 at 161.132 kb on + strand, within Pf1N1B4_5422 at 161.132 kb on + strand, within Pf1N1B4_5422 at 161.132 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.202 kb on - strand, within Pf1N1B4_5422 at 161.236 kb on + strand, within Pf1N1B4_5422 at 161.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 158,982 + -1.0 159,039 + +0.2 159,046 - -2.1 159,136 + -0.9 159,165 - -1.9 159,359 + +0.0 159,465 + -0.0 159,497 + +0.9 159,497 + -1.3 159,497 + -0.8 159,497 + -0.9 159,567 + +0.5 159,685 + -0.9 159,685 + +1.0 159,785 + -0.5 159,785 + -0.0 159,785 + -0.7 159,785 + -2.0 159,785 + +0.1 159,785 + -0.9 159,785 + -0.6 159,785 + -0.5 159,786 - -2.2 159,786 - -0.1 159,786 - -1.2 159,786 - -1.0 159,877 + +0.9 159,877 + +1.1 159,978 + +0.0 159,978 + +0.4 159,978 + +0.2 160,191 + Pf1N1B4_5420 0.69 -0.7 160,192 - Pf1N1B4_5420 0.70 -1.3 160,218 + Pf1N1B4_5420 0.77 -0.3 160,218 + Pf1N1B4_5420 0.77 -0.1 160,219 - Pf1N1B4_5420 0.77 -2.9 160,219 - Pf1N1B4_5420 0.77 -2.2 160,314 + +1.5 160,315 - -0.5 160,317 + -0.3 160,317 + +0.4 160,317 + +0.3 160,318 - -1.0 160,352 - -0.4 160,352 - +0.8 160,370 - +0.5 160,373 + +0.4 160,373 + +0.1 160,373 + +0.7 160,532 - Pf1N1B4_5421 0.48 +0.0 160,636 + +0.8 160,636 + +0.2 160,636 + -0.4 160,636 + +0.9 160,637 - +0.5 160,693 + -0.3 160,694 - +0.6 160,695 + +0.1 160,695 + +1.0 160,695 + -0.7 160,695 + -0.3 160,695 + +0.6 160,696 - +0.8 160,696 - +0.1 160,696 - -0.7 160,696 - -1.0 160,696 - -0.0 160,747 + +0.6 160,747 + +1.1 160,748 - +0.6 160,748 - -0.2 160,748 - +0.5 160,748 - +0.1 160,748 - +0.8 160,749 + +0.5 160,749 + -0.7 160,749 + +0.7 160,749 + +0.4 160,749 + -0.2 160,749 + -0.5 160,750 - -0.2 160,750 - -0.8 160,750 - -0.0 160,750 - -0.3 160,750 - -0.6 160,750 - +1.8 160,774 + -0.3 160,774 + -0.1 160,804 + +1.0 160,823 + -0.7 160,851 + +1.2 161,100 + Pf1N1B4_5422 0.18 -0.1 161,132 + Pf1N1B4_5422 0.19 -0.7 161,132 + Pf1N1B4_5422 0.19 +0.2 161,132 + Pf1N1B4_5422 0.19 -0.2 161,132 + Pf1N1B4_5422 0.19 -0.8 161,201 + Pf1N1B4_5422 0.23 +0.6 161,201 + -0.7 161,201 + Pf1N1B4_5422 0.23 +0.1 161,201 + Pf1N1B4_5422 0.23 +1.0 161,201 + Pf1N1B4_5422 0.23 -0.3 161,201 + Pf1N1B4_5422 0.23 -0.1 161,201 + Pf1N1B4_5422 0.23 -0.1 161,202 - Pf1N1B4_5422 0.23 -2.6 161,236 + Pf1N1B4_5422 0.25 -0.4 161,237 - -0.5
Or see this region's nucleotide sequence