Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5260 and Pf1N1B4_5261 are separated by 280 nucleotides Pf1N1B4_5261 and Pf1N1B4_5262 are separated by 372 nucleotides
Pf1N1B4_5260 - hypothetical protein, at 19,541 to 20,695
_5260
Pf1N1B4_5261 - hypothetical protein, at 20,976 to 21,173
_5261
Pf1N1B4_5262 - hypothetical protein, at 21,546 to 22,034
_5262
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-1
0
1 at 20.274 kb on - strand, within Pf1N1B4_5260 at 20.277 kb on + strand at 20.281 kb on + strand, within Pf1N1B4_5260 at 20.285 kb on - strand, within Pf1N1B4_5260 at 20.627 kb on + strand at 20.627 kb on + strand at 20.630 kb on + strand at 20.657 kb on + strand at 20.758 kb on + strand at 20.977 kb on + strand at 20.977 kb on + strand at 21.099 kb on + strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.200 kb on + strand at 21.220 kb on + strand at 21.359 kb on + strand at 21.360 kb on - strand at 21.589 kb on + strand at 21.724 kb on + strand, within Pf1N1B4_5262 at 21.725 kb on - strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.943 kb on + strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.961 kb on + strand, within Pf1N1B4_5262 at 22.078 kb on - strand at 22.104 kb on + strand at 22.105 kb on - strand at 22.105 kb on - strand at 22.159 kb on + strand at 22.159 kb on + strand at 22.160 kb on - strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 20,274 - Pf1N1B4_5260 0.63 +0.9 20,277 + +1.5 20,281 + Pf1N1B4_5260 0.64 +0.2 20,285 - Pf1N1B4_5260 0.64 -0.4 20,627 + -1.2 20,627 + -0.3 20,630 + +0.1 20,657 + -0.2 20,758 + -0.2 20,977 + +1.2 20,977 + -0.2 21,099 + Pf1N1B4_5261 0.62 -0.4 21,100 - Pf1N1B4_5261 0.63 -0.7 21,100 - Pf1N1B4_5261 0.63 -0.5 21,100 - Pf1N1B4_5261 0.63 -1.1 21,200 + -0.4 21,220 + -0.5 21,359 + +1.2 21,360 - -0.5 21,589 + +0.4 21,724 + Pf1N1B4_5262 0.36 +0.8 21,725 - Pf1N1B4_5262 0.37 -0.5 21,904 + Pf1N1B4_5262 0.73 -0.2 21,904 + Pf1N1B4_5262 0.73 +0.6 21,904 + Pf1N1B4_5262 0.73 -0.4 21,905 - Pf1N1B4_5262 0.73 -0.7 21,905 - Pf1N1B4_5262 0.73 -0.5 21,905 - Pf1N1B4_5262 0.73 +0.1 21,905 - Pf1N1B4_5262 0.73 +0.3 21,941 + Pf1N1B4_5262 0.81 +0.6 21,941 + Pf1N1B4_5262 0.81 -0.6 21,943 + Pf1N1B4_5262 0.81 -1.1 21,944 - Pf1N1B4_5262 0.81 +0.2 21,944 - Pf1N1B4_5262 0.81 +0.5 21,961 + Pf1N1B4_5262 0.85 +0.2 22,078 - +1.2 22,104 + -1.1 22,105 - -0.9 22,105 - +1.1 22,159 + -0.5 22,159 + -0.6 22,160 - -0.4 22,162 + +0.2 22,162 + -0.8 22,162 + -0.5 22,162 + +0.1 22,162 + -0.0
Or see this region's nucleotide sequence