Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4357 and Pf1N1B4_4358 are separated by 405 nucleotides Pf1N1B4_4358 and Pf1N1B4_4359 are separated by 180 nucleotides Pf1N1B4_4359 and Pf1N1B4_4360 are separated by 11 nucleotides Pf1N1B4_4360 and Pf1N1B4_4361 are separated by 27 nucleotides
Pf1N1B4_4357 - Putrescine utilization regulator, at 4,815,395 to 4,815,943
_4357
Pf1N1B4_4358 - Acetyltransferase, GNAT family, at 4,816,349 to 4,816,711
_4358
Pf1N1B4_4359 - PvcA protein, related to known isonitrile synthases, at 4,816,892 to 4,817,887
_4359
Pf1N1B4_4360 - Phosphoglycolate phosphatase (EC 3.1.3.18), at 4,817,899 to 4,818,603
_4360
Pf1N1B4_4361 - PvcB protein, related to amino acid oxidizing enzymes, at 4,818,631 to 4,820,391
_4361
Position (kb)
4816
4817
4818 Strain fitness (log2 ratio)
-1
0
1 at 4816.008 kb on + strand at 4816.169 kb on + strand at 4816.169 kb on + strand at 4816.170 kb on - strand at 4816.170 kb on - strand at 4816.209 kb on - strand at 4816.209 kb on - strand at 4816.334 kb on + strand at 4816.335 kb on - strand at 4816.337 kb on - strand at 4816.386 kb on - strand, within Pf1N1B4_4358 at 4816.538 kb on + strand, within Pf1N1B4_4358 at 4816.648 kb on + strand, within Pf1N1B4_4358 at 4816.648 kb on + strand, within Pf1N1B4_4358 at 4816.652 kb on + strand, within Pf1N1B4_4358 at 4816.728 kb on + strand at 4816.840 kb on + strand at 4816.892 kb on - strand at 4816.892 kb on - strand at 4817.202 kb on + strand, within Pf1N1B4_4359 at 4817.202 kb on + strand, within Pf1N1B4_4359 at 4817.203 kb on - strand, within Pf1N1B4_4359 at 4817.203 kb on - strand at 4817.203 kb on - strand at 4817.234 kb on + strand, within Pf1N1B4_4359 at 4817.234 kb on + strand, within Pf1N1B4_4359 at 4817.234 kb on + strand, within Pf1N1B4_4359 at 4817.235 kb on - strand, within Pf1N1B4_4359 at 4817.273 kb on + strand, within Pf1N1B4_4359 at 4817.274 kb on - strand at 4817.321 kb on + strand, within Pf1N1B4_4359 at 4817.510 kb on + strand, within Pf1N1B4_4359 at 4817.544 kb on - strand, within Pf1N1B4_4359 at 4817.559 kb on + strand, within Pf1N1B4_4359 at 4817.871 kb on - strand at 4817.915 kb on + strand at 4818.031 kb on + strand, within Pf1N1B4_4360 at 4818.115 kb on + strand, within Pf1N1B4_4360 at 4818.439 kb on + strand, within Pf1N1B4_4360 at 4818.591 kb on + strand at 4818.598 kb on + strand at 4818.599 kb on - strand at 4818.599 kb on - strand at 4818.605 kb on - strand at 4818.639 kb on + strand at 4818.640 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 4,816,008 + -0.5 4,816,169 + +0.4 4,816,169 + +1.3 4,816,170 - -0.2 4,816,170 - -0.5 4,816,209 - +0.0 4,816,209 - -0.2 4,816,334 + -1.3 4,816,335 - +0.3 4,816,337 - -0.6 4,816,386 - Pf1N1B4_4358 0.10 -0.0 4,816,538 + Pf1N1B4_4358 0.52 +0.5 4,816,648 + Pf1N1B4_4358 0.82 -0.3 4,816,648 + Pf1N1B4_4358 0.82 +0.2 4,816,652 + Pf1N1B4_4358 0.83 -0.2 4,816,728 + -0.2 4,816,840 + -0.8 4,816,892 - -1.1 4,816,892 - +0.1 4,817,202 + Pf1N1B4_4359 0.31 -1.3 4,817,202 + Pf1N1B4_4359 0.31 -0.4 4,817,203 - Pf1N1B4_4359 0.31 +0.9 4,817,203 - +0.7 4,817,203 - +0.3 4,817,234 + Pf1N1B4_4359 0.34 -1.2 4,817,234 + Pf1N1B4_4359 0.34 -1.2 4,817,234 + Pf1N1B4_4359 0.34 +1.3 4,817,235 - Pf1N1B4_4359 0.34 +0.1 4,817,273 + Pf1N1B4_4359 0.38 -0.2 4,817,274 - -1.1 4,817,321 + Pf1N1B4_4359 0.43 -0.5 4,817,510 + Pf1N1B4_4359 0.62 -0.4 4,817,544 - Pf1N1B4_4359 0.65 +0.1 4,817,559 + Pf1N1B4_4359 0.67 +0.3 4,817,871 - -0.7 4,817,915 + +0.3 4,818,031 + Pf1N1B4_4360 0.19 -0.4 4,818,115 + Pf1N1B4_4360 0.31 -1.4 4,818,439 + Pf1N1B4_4360 0.77 -0.7 4,818,591 + -0.1 4,818,598 + +0.0 4,818,599 - +0.0 4,818,599 - +0.1 4,818,605 - -0.1 4,818,639 + -0.1 4,818,640 - -0.8
Or see this region's nucleotide sequence