Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4195 and Pf1N1B4_4196 overlap by 37 nucleotides Pf1N1B4_4196 and Pf1N1B4_4197 overlap by 2 nucleotides Pf1N1B4_4197 and Pf1N1B4_4198 are separated by 282 nucleotides Pf1N1B4_4198 and Pf1N1B4_4199 are separated by 24 nucleotides
Pf1N1B4_4195 - Transcriptional regulator, TetR family, at 4,647,933 to 4,648,505
_4195
Pf1N1B4_4196 - hypothetical protein, at 4,648,469 to 4,648,630
_4196
Pf1N1B4_4197 - Glucose ABC transport system, periplasmic sugar-binding protein, at 4,648,629 to 4,648,754
_4197
Pf1N1B4_4198 - 4-hydroxybenzoate transporter, at 4,649,037 to 4,650,374
_4198
Pf1N1B4_4199 - Protocatechuate 4,5-dioxygenase beta chain (EC 1.13.11.8), at 4,650,399 to 4,651,661
_4199
Position (kb)
4649
4650
4651 Strain fitness (log2 ratio)
-2
-1
0
1 at 4648.185 kb on + strand at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand at 4648.185 kb on + strand, within Pf1N1B4_4195 at 4648.185 kb on + strand at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.186 kb on - strand, within Pf1N1B4_4195 at 4648.329 kb on + strand, within Pf1N1B4_4195 at 4648.800 kb on + strand at 4648.874 kb on - strand at 4648.952 kb on + strand at 4648.952 kb on + strand at 4649.015 kb on - strand at 4649.022 kb on + strand at 4649.022 kb on + strand at 4649.117 kb on - strand at 4649.128 kb on + strand at 4649.692 kb on + strand, within Pf1N1B4_4198 at 4649.826 kb on + strand, within Pf1N1B4_4198 at 4649.826 kb on + strand, within Pf1N1B4_4198 at 4649.850 kb on + strand, within Pf1N1B4_4198 at 4649.859 kb on - strand, within Pf1N1B4_4198 at 4650.558 kb on + strand, within Pf1N1B4_4199 at 4650.559 kb on - strand, within Pf1N1B4_4199 at 4651.183 kb on - strand, within Pf1N1B4_4199 at 4651.183 kb on - strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 4,648,185 + -1.3 4,648,185 + Pf1N1B4_4195 0.44 +0.3 4,648,185 + Pf1N1B4_4195 0.44 +0.3 4,648,185 + Pf1N1B4_4195 0.44 -1.8 4,648,185 + Pf1N1B4_4195 0.44 -1.0 4,648,185 + Pf1N1B4_4195 0.44 -0.6 4,648,185 + Pf1N1B4_4195 0.44 -0.5 4,648,185 + -0.0 4,648,185 + Pf1N1B4_4195 0.44 -0.4 4,648,185 + +1.1 4,648,186 - Pf1N1B4_4195 0.44 -1.5 4,648,186 - Pf1N1B4_4195 0.44 -0.3 4,648,186 - Pf1N1B4_4195 0.44 +1.0 4,648,186 - -0.4 4,648,186 - Pf1N1B4_4195 0.44 -0.2 4,648,186 - Pf1N1B4_4195 0.44 -0.7 4,648,329 + Pf1N1B4_4195 0.69 +0.6 4,648,800 + -0.7 4,648,874 - -0.9 4,648,952 + +0.1 4,648,952 + +0.7 4,649,015 - +1.1 4,649,022 + +0.3 4,649,022 + +0.8 4,649,117 - +0.2 4,649,128 + +0.1 4,649,692 + Pf1N1B4_4198 0.49 -1.2 4,649,826 + Pf1N1B4_4198 0.59 -0.6 4,649,826 + Pf1N1B4_4198 0.59 -0.2 4,649,850 + Pf1N1B4_4198 0.61 +0.5 4,649,859 - Pf1N1B4_4198 0.61 -1.2 4,650,558 + Pf1N1B4_4199 0.13 +0.4 4,650,559 - Pf1N1B4_4199 0.13 +0.0 4,651,183 - Pf1N1B4_4199 0.62 -1.6 4,651,183 - Pf1N1B4_4199 0.62 -0.4 4,651,185 + Pf1N1B4_4199 0.62 -1.8 4,651,185 + Pf1N1B4_4199 0.62 +0.2 4,651,185 + Pf1N1B4_4199 0.62 +0.2
Or see this region's nucleotide sequence