Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3881 and Pf1N1B4_3882 overlap by 4 nucleotides Pf1N1B4_3882 and Pf1N1B4_3883 are separated by 45 nucleotides Pf1N1B4_3883 and Pf1N1B4_3884 are separated by 10 nucleotides Pf1N1B4_3884 and Pf1N1B4_3885 are separated by 108 nucleotides Pf1N1B4_3885 and Pf1N1B4_3886 are separated by 206 nucleotides
Pf1N1B4_3881 - Cytochrome c oxidase subunit CcoP (EC 1.9.3.1), at 4,310,112 to 4,311,065
_3881
Pf1N1B4_3882 - Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1), at 4,311,062 to 4,311,238
_3882
Pf1N1B4_3883 - Cytochrome c oxidase subunit CcoO (EC 1.9.3.1), at 4,311,284 to 4,311,892
_3883
Pf1N1B4_3884 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 4,311,903 to 4,313,327
_3884
Pf1N1B4_3885 - Predicted hydrolase of the alpha/beta-hydrolase fold, at 4,313,436 to 4,314,113
_3885
Pf1N1B4_3886 - Aerotaxis receptor Aer, at 4,314,320 to 4,315,885
_3886
Position (kb)
4311
4312
4313
4314 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4311.047 kb on + strand at 4311.048 kb on - strand at 4311.251 kb on + strand at 4311.252 kb on - strand at 4311.258 kb on - strand at 4311.325 kb on - strand at 4311.325 kb on - strand at 4311.397 kb on - strand, within Pf1N1B4_3883 at 4311.478 kb on - strand, within Pf1N1B4_3883 at 4311.519 kb on + strand, within Pf1N1B4_3883 at 4311.519 kb on + strand, within Pf1N1B4_3883 at 4311.520 kb on - strand, within Pf1N1B4_3883 at 4311.520 kb on - strand, within Pf1N1B4_3883 at 4311.520 kb on - strand at 4311.520 kb on - strand, within Pf1N1B4_3883 at 4311.639 kb on + strand, within Pf1N1B4_3883 at 4311.717 kb on + strand, within Pf1N1B4_3883 at 4311.717 kb on + strand, within Pf1N1B4_3883 at 4311.717 kb on + strand, within Pf1N1B4_3883 at 4311.717 kb on + strand, within Pf1N1B4_3883 at 4311.717 kb on + strand, within Pf1N1B4_3883 at 4311.717 kb on + strand, within Pf1N1B4_3883 at 4311.717 kb on + strand, within Pf1N1B4_3883 at 4311.718 kb on - strand, within Pf1N1B4_3883 at 4311.718 kb on - strand, within Pf1N1B4_3883 at 4311.750 kb on + strand, within Pf1N1B4_3883 at 4311.751 kb on - strand, within Pf1N1B4_3883 at 4311.970 kb on + strand at 4311.970 kb on + strand at 4311.971 kb on - strand at 4311.971 kb on - strand at 4311.971 kb on - strand at 4312.066 kb on + strand, within Pf1N1B4_3884 at 4312.066 kb on + strand, within Pf1N1B4_3884 at 4312.067 kb on - strand, within Pf1N1B4_3884 at 4312.067 kb on - strand, within Pf1N1B4_3884 at 4312.067 kb on - strand, within Pf1N1B4_3884 at 4312.067 kb on - strand, within Pf1N1B4_3884 at 4312.067 kb on - strand, within Pf1N1B4_3884 at 4312.144 kb on + strand, within Pf1N1B4_3884 at 4312.144 kb on + strand at 4312.145 kb on - strand, within Pf1N1B4_3884 at 4312.145 kb on - strand at 4312.145 kb on - strand, within Pf1N1B4_3884 at 4312.316 kb on - strand, within Pf1N1B4_3884 at 4312.316 kb on - strand, within Pf1N1B4_3884 at 4312.379 kb on - strand, within Pf1N1B4_3884 at 4312.430 kb on + strand, within Pf1N1B4_3884 at 4312.430 kb on + strand, within Pf1N1B4_3884 at 4312.431 kb on - strand, within Pf1N1B4_3884 at 4312.431 kb on - strand, within Pf1N1B4_3884 at 4312.431 kb on - strand, within Pf1N1B4_3884 at 4312.546 kb on + strand, within Pf1N1B4_3884 at 4312.547 kb on - strand, within Pf1N1B4_3884 at 4312.547 kb on - strand, within Pf1N1B4_3884 at 4312.577 kb on - strand, within Pf1N1B4_3884 at 4312.577 kb on - strand, within Pf1N1B4_3884 at 4312.577 kb on - strand, within Pf1N1B4_3884 at 4312.577 kb on - strand, within Pf1N1B4_3884 at 4312.577 kb on - strand, within Pf1N1B4_3884 at 4312.577 kb on - strand, within Pf1N1B4_3884 at 4312.577 kb on - strand, within Pf1N1B4_3884 at 4312.624 kb on + strand, within Pf1N1B4_3884 at 4312.624 kb on + strand, within Pf1N1B4_3884 at 4312.624 kb on + strand, within Pf1N1B4_3884 at 4312.624 kb on + strand, within Pf1N1B4_3884 at 4312.624 kb on + strand, within Pf1N1B4_3884 at 4312.657 kb on + strand, within Pf1N1B4_3884 at 4312.657 kb on + strand, within Pf1N1B4_3884 at 4312.657 kb on + strand, within Pf1N1B4_3884 at 4312.657 kb on + strand, within Pf1N1B4_3884 at 4312.658 kb on - strand, within Pf1N1B4_3884 at 4312.658 kb on - strand, within Pf1N1B4_3884 at 4312.658 kb on - strand, within Pf1N1B4_3884 at 4312.658 kb on - strand, within Pf1N1B4_3884 at 4312.660 kb on + strand, within Pf1N1B4_3884 at 4312.660 kb on + strand, within Pf1N1B4_3884 at 4312.660 kb on + strand, within Pf1N1B4_3884 at 4312.660 kb on + strand, within Pf1N1B4_3884 at 4312.661 kb on - strand, within Pf1N1B4_3884 at 4312.661 kb on - strand, within Pf1N1B4_3884 at 4312.661 kb on - strand, within Pf1N1B4_3884 at 4312.661 kb on - strand, within Pf1N1B4_3884 at 4312.661 kb on - strand, within Pf1N1B4_3884 at 4312.750 kb on + strand, within Pf1N1B4_3884 at 4312.760 kb on - strand at 4312.855 kb on + strand, within Pf1N1B4_3884 at 4312.855 kb on + strand, within Pf1N1B4_3884 at 4312.855 kb on + strand, within Pf1N1B4_3884 at 4312.855 kb on + strand, within Pf1N1B4_3884 at 4312.855 kb on + strand, within Pf1N1B4_3884 at 4312.855 kb on + strand, within Pf1N1B4_3884 at 4312.855 kb on + strand, within Pf1N1B4_3884 at 4312.856 kb on - strand, within Pf1N1B4_3884 at 4312.856 kb on - strand, within Pf1N1B4_3884 at 4312.856 kb on - strand, within Pf1N1B4_3884 at 4312.856 kb on - strand, within Pf1N1B4_3884 at 4312.856 kb on - strand, within Pf1N1B4_3884 at 4312.856 kb on - strand, within Pf1N1B4_3884 at 4312.856 kb on - strand, within Pf1N1B4_3884 at 4312.856 kb on - strand, within Pf1N1B4_3884 at 4312.856 kb on - strand at 4312.856 kb on - strand, within Pf1N1B4_3884 at 4312.906 kb on + strand at 4312.906 kb on + strand, within Pf1N1B4_3884 at 4312.906 kb on + strand, within Pf1N1B4_3884 at 4312.907 kb on - strand, within Pf1N1B4_3884 at 4313.086 kb on - strand, within Pf1N1B4_3884 at 4313.092 kb on + strand, within Pf1N1B4_3884 at 4313.092 kb on + strand, within Pf1N1B4_3884 at 4313.092 kb on + strand, within Pf1N1B4_3884 at 4313.093 kb on - strand, within Pf1N1B4_3884 at 4313.093 kb on - strand, within Pf1N1B4_3884 at 4313.093 kb on - strand, within Pf1N1B4_3884 at 4313.093 kb on - strand, within Pf1N1B4_3884 at 4313.144 kb on + strand, within Pf1N1B4_3884 at 4313.144 kb on + strand, within Pf1N1B4_3884 at 4313.270 kb on + strand at 4313.270 kb on + strand at 4313.296 kb on + strand at 4313.296 kb on + strand at 4313.297 kb on - strand at 4313.297 kb on - strand at 4313.297 kb on - strand at 4313.297 kb on - strand at 4313.297 kb on - strand at 4313.302 kb on + strand at 4313.315 kb on + strand at 4313.315 kb on + strand at 4313.315 kb on + strand at 4313.315 kb on + strand at 4313.315 kb on + strand at 4313.376 kb on - strand at 4313.409 kb on + strand at 4313.409 kb on + strand at 4313.409 kb on + strand at 4313.411 kb on + strand at 4313.411 kb on + strand at 4313.411 kb on + strand at 4313.411 kb on + strand at 4313.412 kb on - strand at 4313.412 kb on - strand at 4313.412 kb on - strand at 4313.412 kb on - strand at 4313.412 kb on - strand at 4313.412 kb on - strand at 4313.412 kb on - strand at 4313.412 kb on - strand at 4313.420 kb on + strand at 4313.421 kb on - strand at 4313.649 kb on + strand, within Pf1N1B4_3885 at 4313.649 kb on + strand, within Pf1N1B4_3885 at 4313.730 kb on + strand, within Pf1N1B4_3885 at 4313.730 kb on + strand, within Pf1N1B4_3885 at 4313.730 kb on + strand, within Pf1N1B4_3885 at 4313.730 kb on + strand, within Pf1N1B4_3885 at 4313.730 kb on + strand, within Pf1N1B4_3885 at 4313.731 kb on - strand, within Pf1N1B4_3885 at 4313.731 kb on - strand at 4313.731 kb on - strand, within Pf1N1B4_3885 at 4313.731 kb on - strand, within Pf1N1B4_3885 at 4313.731 kb on - strand, within Pf1N1B4_3885 at 4313.859 kb on + strand, within Pf1N1B4_3885 at 4313.859 kb on + strand, within Pf1N1B4_3885 at 4313.859 kb on + strand, within Pf1N1B4_3885 at 4313.977 kb on - strand, within Pf1N1B4_3885 at 4313.977 kb on - strand, within Pf1N1B4_3885 at 4314.056 kb on + strand at 4314.057 kb on - strand at 4314.177 kb on + strand at 4314.205 kb on + strand at 4314.206 kb on - strand at 4314.269 kb on + strand at 4314.316 kb on + strand at 4314.316 kb on + strand at 4314.317 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 4,311,047 + -1.0 4,311,048 - -0.0 4,311,251 + -1.0 4,311,252 - -1.2 4,311,258 - -0.5 4,311,325 - -1.1 4,311,325 - +0.5 4,311,397 - Pf1N1B4_3883 0.19 -2.3 4,311,478 - Pf1N1B4_3883 0.32 -0.9 4,311,519 + Pf1N1B4_3883 0.39 -1.6 4,311,519 + Pf1N1B4_3883 0.39 -1.2 4,311,520 - Pf1N1B4_3883 0.39 -1.6 4,311,520 - Pf1N1B4_3883 0.39 -0.5 4,311,520 - +0.5 4,311,520 - Pf1N1B4_3883 0.39 -2.1 4,311,639 + Pf1N1B4_3883 0.58 -1.4 4,311,717 + Pf1N1B4_3883 0.71 -0.9 4,311,717 + Pf1N1B4_3883 0.71 -1.1 4,311,717 + Pf1N1B4_3883 0.71 -0.9 4,311,717 + Pf1N1B4_3883 0.71 -0.2 4,311,717 + Pf1N1B4_3883 0.71 -0.8 4,311,717 + Pf1N1B4_3883 0.71 +0.5 4,311,717 + Pf1N1B4_3883 0.71 -1.3 4,311,718 - Pf1N1B4_3883 0.71 -0.7 4,311,718 - Pf1N1B4_3883 0.71 -0.6 4,311,750 + Pf1N1B4_3883 0.77 -3.1 4,311,751 - Pf1N1B4_3883 0.77 -0.4 4,311,970 + -2.1 4,311,970 + -2.7 4,311,971 - -0.8 4,311,971 - -1.4 4,311,971 - -2.6 4,312,066 + Pf1N1B4_3884 0.11 -2.4 4,312,066 + Pf1N1B4_3884 0.11 -1.4 4,312,067 - Pf1N1B4_3884 0.12 -1.2 4,312,067 - Pf1N1B4_3884 0.12 +0.0 4,312,067 - Pf1N1B4_3884 0.12 -2.2 4,312,067 - Pf1N1B4_3884 0.12 -2.1 4,312,067 - Pf1N1B4_3884 0.12 -0.4 4,312,144 + Pf1N1B4_3884 0.17 -2.1 4,312,144 + -0.1 4,312,145 - Pf1N1B4_3884 0.17 -1.1 4,312,145 - +0.1 4,312,145 - Pf1N1B4_3884 0.17 -1.2 4,312,316 - Pf1N1B4_3884 0.29 -1.1 4,312,316 - Pf1N1B4_3884 0.29 -1.7 4,312,379 - Pf1N1B4_3884 0.33 -0.2 4,312,430 + Pf1N1B4_3884 0.37 -1.2 4,312,430 + Pf1N1B4_3884 0.37 -3.6 4,312,431 - Pf1N1B4_3884 0.37 -1.3 4,312,431 - Pf1N1B4_3884 0.37 -1.5 4,312,431 - Pf1N1B4_3884 0.37 -1.5 4,312,546 + Pf1N1B4_3884 0.45 -1.0 4,312,547 - Pf1N1B4_3884 0.45 -1.9 4,312,547 - Pf1N1B4_3884 0.45 -0.5 4,312,577 - Pf1N1B4_3884 0.47 -1.0 4,312,577 - Pf1N1B4_3884 0.47 -0.7 4,312,577 - Pf1N1B4_3884 0.47 -2.2 4,312,577 - Pf1N1B4_3884 0.47 +0.1 4,312,577 - Pf1N1B4_3884 0.47 -1.0 4,312,577 - Pf1N1B4_3884 0.47 -0.6 4,312,577 - Pf1N1B4_3884 0.47 +0.4 4,312,624 + Pf1N1B4_3884 0.51 -1.2 4,312,624 + Pf1N1B4_3884 0.51 -0.5 4,312,624 + Pf1N1B4_3884 0.51 -1.0 4,312,624 + Pf1N1B4_3884 0.51 -1.2 4,312,624 + Pf1N1B4_3884 0.51 -1.1 4,312,657 + Pf1N1B4_3884 0.53 -1.9 4,312,657 + Pf1N1B4_3884 0.53 -0.5 4,312,657 + Pf1N1B4_3884 0.53 -0.7 4,312,657 + Pf1N1B4_3884 0.53 -0.9 4,312,658 - Pf1N1B4_3884 0.53 -1.6 4,312,658 - Pf1N1B4_3884 0.53 -2.6 4,312,658 - Pf1N1B4_3884 0.53 -2.3 4,312,658 - Pf1N1B4_3884 0.53 -1.9 4,312,660 + Pf1N1B4_3884 0.53 +0.5 4,312,660 + Pf1N1B4_3884 0.53 -2.1 4,312,660 + Pf1N1B4_3884 0.53 -1.6 4,312,660 + Pf1N1B4_3884 0.53 -1.5 4,312,661 - Pf1N1B4_3884 0.53 -0.1 4,312,661 - Pf1N1B4_3884 0.53 -2.5 4,312,661 - Pf1N1B4_3884 0.53 -1.2 4,312,661 - Pf1N1B4_3884 0.53 +0.0 4,312,661 - Pf1N1B4_3884 0.53 -0.6 4,312,750 + Pf1N1B4_3884 0.59 -1.0 4,312,760 - +1.5 4,312,855 + Pf1N1B4_3884 0.67 -1.1 4,312,855 + Pf1N1B4_3884 0.67 -1.3 4,312,855 + Pf1N1B4_3884 0.67 +0.1 4,312,855 + Pf1N1B4_3884 0.67 -1.2 4,312,855 + Pf1N1B4_3884 0.67 -0.3 4,312,855 + Pf1N1B4_3884 0.67 -1.8 4,312,855 + Pf1N1B4_3884 0.67 -0.1 4,312,856 - Pf1N1B4_3884 0.67 -1.2 4,312,856 - Pf1N1B4_3884 0.67 -0.2 4,312,856 - Pf1N1B4_3884 0.67 -3.5 4,312,856 - Pf1N1B4_3884 0.67 -1.1 4,312,856 - Pf1N1B4_3884 0.67 -2.8 4,312,856 - Pf1N1B4_3884 0.67 -0.3 4,312,856 - Pf1N1B4_3884 0.67 -2.8 4,312,856 - Pf1N1B4_3884 0.67 -1.4 4,312,856 - -1.1 4,312,856 - Pf1N1B4_3884 0.67 -0.4 4,312,906 + -1.5 4,312,906 + Pf1N1B4_3884 0.70 -1.2 4,312,906 + Pf1N1B4_3884 0.70 -0.9 4,312,907 - Pf1N1B4_3884 0.70 -0.8 4,313,086 - Pf1N1B4_3884 0.83 -1.2 4,313,092 + Pf1N1B4_3884 0.83 -0.8 4,313,092 + Pf1N1B4_3884 0.83 -1.2 4,313,092 + Pf1N1B4_3884 0.83 -0.6 4,313,093 - Pf1N1B4_3884 0.84 -2.3 4,313,093 - Pf1N1B4_3884 0.84 +0.0 4,313,093 - Pf1N1B4_3884 0.84 -2.7 4,313,093 - Pf1N1B4_3884 0.84 -0.8 4,313,144 + Pf1N1B4_3884 0.87 -0.5 4,313,144 + Pf1N1B4_3884 0.87 -1.9 4,313,270 + -1.3 4,313,270 + +0.5 4,313,296 + -1.4 4,313,296 + -0.6 4,313,297 - -1.7 4,313,297 - -0.5 4,313,297 - -1.9 4,313,297 - +0.4 4,313,297 - -1.0 4,313,302 + -0.9 4,313,315 + -1.1 4,313,315 + +0.1 4,313,315 + -1.0 4,313,315 + -1.4 4,313,315 + -0.7 4,313,376 - -0.5 4,313,409 + -2.5 4,313,409 + -0.4 4,313,409 + -1.9 4,313,411 + -0.7 4,313,411 + -0.7 4,313,411 + -1.1 4,313,411 + -1.4 4,313,412 - -0.4 4,313,412 - -1.5 4,313,412 - -1.2 4,313,412 - +0.4 4,313,412 - -0.2 4,313,412 - -1.1 4,313,412 - -0.8 4,313,412 - -0.1 4,313,420 + +0.4 4,313,421 - -2.0 4,313,649 + Pf1N1B4_3885 0.31 -0.7 4,313,649 + Pf1N1B4_3885 0.31 -0.1 4,313,730 + Pf1N1B4_3885 0.43 -0.1 4,313,730 + Pf1N1B4_3885 0.43 -0.6 4,313,730 + Pf1N1B4_3885 0.43 +0.5 4,313,730 + Pf1N1B4_3885 0.43 +0.9 4,313,730 + Pf1N1B4_3885 0.43 -0.2 4,313,731 - Pf1N1B4_3885 0.44 +0.5 4,313,731 - +0.7 4,313,731 - Pf1N1B4_3885 0.44 +0.4 4,313,731 - Pf1N1B4_3885 0.44 -0.7 4,313,731 - Pf1N1B4_3885 0.44 -1.0 4,313,859 + Pf1N1B4_3885 0.62 +0.4 4,313,859 + Pf1N1B4_3885 0.62 -0.6 4,313,859 + Pf1N1B4_3885 0.62 -1.0 4,313,977 - Pf1N1B4_3885 0.80 +0.1 4,313,977 - Pf1N1B4_3885 0.80 -2.5 4,314,056 + -0.5 4,314,057 - +2.0 4,314,177 + -1.2 4,314,205 + -2.5 4,314,206 - -0.9 4,314,269 + +0.1 4,314,316 + -0.5 4,314,316 + -0.6 4,314,317 - -0.8
Or see this region's nucleotide sequence