Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3484 and Pf1N1B4_3485 are separated by 486 nucleotides Pf1N1B4_3485 and Pf1N1B4_3486 are separated by 126 nucleotides Pf1N1B4_3486 and Pf1N1B4_3487 are separated by 340 nucleotides
Pf1N1B4_3484 - Sensor protein PhoQ (EC 2.7.13.3), at 3,890,692 to 3,892,038
_3484
Pf1N1B4_3485 - hypothetical protein, at 3,892,525 to 3,892,884
_3485
Pf1N1B4_3486 - Transcriptional regulator, AraC family, at 3,893,011 to 3,894,024
_3486
Pf1N1B4_3487 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, aspartate, at 3,894,365 to 3,895,699
_3487
Position (kb)
3893
3894
3895 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3892.163 kb on + strand at 3892.178 kb on - strand at 3892.194 kb on + strand at 3892.235 kb on + strand at 3892.277 kb on + strand at 3892.277 kb on + strand at 3892.277 kb on + strand at 3892.278 kb on - strand at 3892.371 kb on + strand at 3892.459 kb on + strand at 3892.474 kb on + strand at 3892.474 kb on + strand at 3892.474 kb on + strand at 3892.475 kb on + strand at 3892.475 kb on - strand at 3892.488 kb on + strand at 3892.537 kb on - strand at 3892.537 kb on - strand at 3892.627 kb on + strand, within Pf1N1B4_3485 at 3892.627 kb on + strand, within Pf1N1B4_3485 at 3892.630 kb on + strand at 3892.634 kb on + strand at 3892.634 kb on + strand, within Pf1N1B4_3485 at 3892.682 kb on - strand, within Pf1N1B4_3485 at 3892.682 kb on - strand, within Pf1N1B4_3485 at 3892.682 kb on - strand, within Pf1N1B4_3485 at 3893.147 kb on + strand, within Pf1N1B4_3486 at 3893.148 kb on - strand, within Pf1N1B4_3486 at 3893.399 kb on - strand, within Pf1N1B4_3486 at 3893.501 kb on + strand, within Pf1N1B4_3486 at 3893.718 kb on - strand, within Pf1N1B4_3486 at 3893.828 kb on + strand, within Pf1N1B4_3486 at 3893.828 kb on + strand, within Pf1N1B4_3486 at 3893.829 kb on - strand, within Pf1N1B4_3486 at 3893.829 kb on - strand, within Pf1N1B4_3486 at 3893.829 kb on - strand, within Pf1N1B4_3486 at 3894.024 kb on + strand at 3894.025 kb on - strand at 3894.177 kb on + strand at 3894.338 kb on + strand at 3894.338 kb on + strand at 3894.362 kb on - strand at 3894.362 kb on - strand at 3894.362 kb on - strand at 3894.386 kb on + strand at 3894.387 kb on - strand at 3894.388 kb on + strand at 3894.389 kb on - strand at 3894.399 kb on - strand at 3894.536 kb on - strand, within Pf1N1B4_3487 at 3894.608 kb on + strand at 3894.609 kb on - strand, within Pf1N1B4_3487 at 3894.609 kb on - strand, within Pf1N1B4_3487 at 3895.001 kb on + strand, within Pf1N1B4_3487
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 3,892,163 + +0.6 3,892,178 - -0.5 3,892,194 + +0.5 3,892,235 + -0.2 3,892,277 + +0.9 3,892,277 + -0.7 3,892,277 + -0.8 3,892,278 - -0.1 3,892,371 + +0.1 3,892,459 + +0.7 3,892,474 + +0.2 3,892,474 + -0.4 3,892,474 + +1.0 3,892,475 + -1.8 3,892,475 - -1.0 3,892,488 + -0.9 3,892,537 - +1.8 3,892,537 - +0.2 3,892,627 + Pf1N1B4_3485 0.28 -0.4 3,892,627 + Pf1N1B4_3485 0.28 -0.5 3,892,630 + +1.5 3,892,634 + -0.2 3,892,634 + Pf1N1B4_3485 0.30 -0.4 3,892,682 - Pf1N1B4_3485 0.44 +0.4 3,892,682 - Pf1N1B4_3485 0.44 -0.6 3,892,682 - Pf1N1B4_3485 0.44 +0.0 3,893,147 + Pf1N1B4_3486 0.13 +0.4 3,893,148 - Pf1N1B4_3486 0.14 +0.4 3,893,399 - Pf1N1B4_3486 0.38 -0.2 3,893,501 + Pf1N1B4_3486 0.48 -0.8 3,893,718 - Pf1N1B4_3486 0.70 -0.6 3,893,828 + Pf1N1B4_3486 0.81 -0.9 3,893,828 + Pf1N1B4_3486 0.81 -0.4 3,893,829 - Pf1N1B4_3486 0.81 -0.8 3,893,829 - Pf1N1B4_3486 0.81 -1.9 3,893,829 - Pf1N1B4_3486 0.81 -0.2 3,894,024 + -0.3 3,894,025 - +0.1 3,894,177 + -1.3 3,894,338 + -0.6 3,894,338 + +0.1 3,894,362 - -0.1 3,894,362 - +1.4 3,894,362 - +0.1 3,894,386 + +0.3 3,894,387 - +0.2 3,894,388 + -0.3 3,894,389 - -0.2 3,894,399 - +0.1 3,894,536 - Pf1N1B4_3487 0.13 +1.0 3,894,608 + +1.8 3,894,609 - Pf1N1B4_3487 0.18 -0.3 3,894,609 - Pf1N1B4_3487 0.18 -1.3 3,895,001 + Pf1N1B4_3487 0.48 -0.4
Or see this region's nucleotide sequence