Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_239 and Pf1N1B4_240 are separated by 73 nucleotides Pf1N1B4_240 and Pf1N1B4_241 are separated by 163 nucleotides Pf1N1B4_241 and Pf1N1B4_242 are separated by 192 nucleotides
Pf1N1B4_239 - Similar to ribosomal large subunit pseudouridine synthase A, at 298,039 to 298,929
_239
Pf1N1B4_240 - Transcriptional regulators, at 299,003 to 299,323
_240
Pf1N1B4_241 - Thiol-disulfide isomerase and thioredoxins, at 299,487 to 299,744
_241
Pf1N1B4_242 - Glutathione-regulated potassium-efflux system protein KefB, at 299,937 to 301,649
_242
Position (kb) 299
300 Strain fitness (log2 ratio) -2
-1
0
1 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.736 kb on + strand, within Pf1N1B4_239 at 298.737 kb on - strand at 298.880 kb on + strand at 298.880 kb on + strand at 298.881 kb on - strand at 298.881 kb on - strand at 298.953 kb on - strand at 298.990 kb on + strand at 298.990 kb on + strand at 298.991 kb on - strand at 298.991 kb on - strand at 299.103 kb on + strand, within Pf1N1B4_240 at 299.103 kb on + strand, within Pf1N1B4_240 at 299.103 kb on + strand, within Pf1N1B4_240 at 299.189 kb on - strand, within Pf1N1B4_240 at 299.357 kb on + strand at 299.411 kb on - strand at 299.576 kb on + strand, within Pf1N1B4_241 at 299.587 kb on + strand, within Pf1N1B4_241 at 299.750 kb on + strand at 299.750 kb on + strand at 299.750 kb on + strand at 299.751 kb on - strand at 299.751 kb on - strand at 299.763 kb on + strand at 299.764 kb on - strand at 299.798 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.901 kb on - strand at 299.901 kb on - strand at 299.901 kb on - strand at 300.409 kb on + strand, within Pf1N1B4_242 at 300.456 kb on + strand, within Pf1N1B4_242 at 300.677 kb on - strand, within Pf1N1B4_242 at 300.677 kb on - strand, within Pf1N1B4_242
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 298,713 - Pf1N1B4_239 0.76 -1.4 298,713 - Pf1N1B4_239 0.76 -0.5 298,713 - Pf1N1B4_239 0.76 +0.2 298,736 + Pf1N1B4_239 0.78 -0.7 298,737 - -0.9 298,880 + +0.4 298,880 + -0.1 298,881 - -0.1 298,881 - -1.1 298,953 - -1.5 298,990 + +0.3 298,990 + +0.3 298,991 - -1.1 298,991 - +0.6 299,103 + Pf1N1B4_240 0.31 -0.3 299,103 + Pf1N1B4_240 0.31 -1.2 299,103 + Pf1N1B4_240 0.31 -0.3 299,189 - Pf1N1B4_240 0.58 -0.1 299,357 + -0.5 299,411 - +0.3 299,576 + Pf1N1B4_241 0.34 -1.2 299,587 + Pf1N1B4_241 0.39 -2.0 299,750 + -0.8 299,750 + -1.5 299,750 + -1.0 299,751 - +0.1 299,751 - -0.6 299,763 + +0.3 299,764 - -0.4 299,798 + -1.1 299,900 + -0.6 299,900 + +0.7 299,900 + -0.5 299,900 + +0.2 299,900 + -0.1 299,901 - -0.5 299,901 - -0.4 299,901 - +0.4 300,409 + Pf1N1B4_242 0.28 +0.3 300,456 + Pf1N1B4_242 0.30 -0.6 300,677 - Pf1N1B4_242 0.43 +0.4 300,677 - Pf1N1B4_242 0.43 +0.3
Or see this region's nucleotide sequence