Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2149 and Pf1N1B4_2150 are separated by 127 nucleotides Pf1N1B4_2150 and Pf1N1B4_2151 are separated by 69 nucleotides
Pf1N1B4_2149 - Acetyl-CoA hydrolase, at 2,458,747 to 2,460,669
_2149
Pf1N1B4_2150 - Xanthine phosphoribosyltransferase (EC 2.4.2.22), at 2,460,797 to 2,461,369
_2150
Pf1N1B4_2151 - ATP-dependent DNA helicase Rep, at 2,461,439 to 2,463,448
_2151
Position (kb)
2460
2461
2462 Strain fitness (log2 ratio)
-1
0
1 at 2459.842 kb on + strand, within Pf1N1B4_2149 at 2459.842 kb on + strand, within Pf1N1B4_2149 at 2459.872 kb on - strand, within Pf1N1B4_2149 at 2459.872 kb on - strand, within Pf1N1B4_2149 at 2459.905 kb on + strand, within Pf1N1B4_2149 at 2459.905 kb on + strand, within Pf1N1B4_2149 at 2459.905 kb on + strand, within Pf1N1B4_2149 at 2459.905 kb on + strand, within Pf1N1B4_2149 at 2459.906 kb on - strand, within Pf1N1B4_2149 at 2459.906 kb on - strand, within Pf1N1B4_2149 at 2459.906 kb on - strand, within Pf1N1B4_2149 at 2459.906 kb on - strand, within Pf1N1B4_2149 at 2459.906 kb on - strand, within Pf1N1B4_2149 at 2459.920 kb on + strand, within Pf1N1B4_2149 at 2460.058 kb on + strand, within Pf1N1B4_2149 at 2460.058 kb on + strand, within Pf1N1B4_2149 at 2460.058 kb on + strand, within Pf1N1B4_2149 at 2460.058 kb on + strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.167 kb on - strand, within Pf1N1B4_2149 at 2460.470 kb on + strand, within Pf1N1B4_2149 at 2460.470 kb on + strand at 2460.470 kb on + strand, within Pf1N1B4_2149 at 2460.470 kb on + strand, within Pf1N1B4_2149 at 2460.471 kb on - strand, within Pf1N1B4_2149 at 2460.471 kb on - strand, within Pf1N1B4_2149 at 2460.471 kb on - strand, within Pf1N1B4_2149 at 2460.471 kb on - strand, within Pf1N1B4_2149 at 2460.471 kb on - strand, within Pf1N1B4_2149 at 2460.667 kb on + strand at 2460.667 kb on + strand at 2460.744 kb on + strand at 2460.744 kb on + strand at 2460.745 kb on - strand at 2460.745 kb on - strand at 2460.745 kb on - strand at 2460.813 kb on - strand at 2460.917 kb on - strand, within Pf1N1B4_2150 at 2461.080 kb on + strand, within Pf1N1B4_2150 at 2461.081 kb on - strand, within Pf1N1B4_2150 at 2461.081 kb on - strand, within Pf1N1B4_2150 at 2461.382 kb on + strand at 2461.408 kb on + strand at 2461.430 kb on + strand at 2461.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 2,459,842 + Pf1N1B4_2149 0.57 +0.4 2,459,842 + Pf1N1B4_2149 0.57 +0.2 2,459,872 - Pf1N1B4_2149 0.59 +0.4 2,459,872 - Pf1N1B4_2149 0.59 +0.0 2,459,905 + Pf1N1B4_2149 0.60 -0.9 2,459,905 + Pf1N1B4_2149 0.60 -1.5 2,459,905 + Pf1N1B4_2149 0.60 +0.4 2,459,905 + Pf1N1B4_2149 0.60 -0.9 2,459,906 - Pf1N1B4_2149 0.60 -1.1 2,459,906 - Pf1N1B4_2149 0.60 -0.7 2,459,906 - Pf1N1B4_2149 0.60 +1.1 2,459,906 - Pf1N1B4_2149 0.60 +0.6 2,459,906 - Pf1N1B4_2149 0.60 -0.3 2,459,920 + Pf1N1B4_2149 0.61 +0.2 2,460,058 + Pf1N1B4_2149 0.68 +0.4 2,460,058 + Pf1N1B4_2149 0.68 +0.2 2,460,058 + Pf1N1B4_2149 0.68 +0.3 2,460,058 + Pf1N1B4_2149 0.68 +0.8 2,460,059 - Pf1N1B4_2149 0.68 -0.4 2,460,059 - Pf1N1B4_2149 0.68 -1.7 2,460,059 - Pf1N1B4_2149 0.68 +0.4 2,460,059 - Pf1N1B4_2149 0.68 -0.7 2,460,059 - Pf1N1B4_2149 0.68 +0.1 2,460,059 - Pf1N1B4_2149 0.68 +0.0 2,460,059 - Pf1N1B4_2149 0.68 +0.1 2,460,167 - Pf1N1B4_2149 0.74 +1.5 2,460,470 + Pf1N1B4_2149 0.90 +0.2 2,460,470 + +0.7 2,460,470 + Pf1N1B4_2149 0.90 +0.2 2,460,470 + Pf1N1B4_2149 0.90 -0.2 2,460,471 - Pf1N1B4_2149 0.90 +0.8 2,460,471 - Pf1N1B4_2149 0.90 -0.7 2,460,471 - Pf1N1B4_2149 0.90 -1.5 2,460,471 - Pf1N1B4_2149 0.90 -0.6 2,460,471 - Pf1N1B4_2149 0.90 +0.1 2,460,667 + +1.0 2,460,667 + -0.5 2,460,744 + -0.2 2,460,744 + +0.6 2,460,745 - +1.1 2,460,745 - +1.6 2,460,745 - -0.2 2,460,813 - -0.1 2,460,917 - Pf1N1B4_2150 0.21 -0.8 2,461,080 + Pf1N1B4_2150 0.49 -0.5 2,461,081 - Pf1N1B4_2150 0.50 -1.1 2,461,081 - Pf1N1B4_2150 0.50 -1.3 2,461,382 + +0.8 2,461,408 + +0.7 2,461,430 + -0.0 2,461,449 - -0.3
Or see this region's nucleotide sequence