Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1539 and Pf1N1B4_1540 are separated by 13 nucleotides Pf1N1B4_1540 and Pf1N1B4_1541 overlap by 4 nucleotides Pf1N1B4_1541 and Pf1N1B4_1542 overlap by 4 nucleotides
Pf1N1B4_1539 - Type IV pilus biogenesis protein PilQ, at 1,774,881 to 1,776,956
_1539
Pf1N1B4_1540 - Type IV pilus biogenesis protein PilP, at 1,776,970 to 1,777,497
_1540
Pf1N1B4_1541 - Type IV pilus biogenesis protein PilO, at 1,777,494 to 1,778,117
_1541
Pf1N1B4_1542 - Type IV pilus biogenesis protein PilN, at 1,778,114 to 1,778,680
_1542
Position (kb)
1776
1777
1778 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1776.217 kb on + strand, within Pf1N1B4_1539 at 1776.217 kb on + strand, within Pf1N1B4_1539 at 1776.217 kb on + strand, within Pf1N1B4_1539 at 1776.218 kb on - strand, within Pf1N1B4_1539 at 1776.226 kb on + strand, within Pf1N1B4_1539 at 1776.330 kb on + strand, within Pf1N1B4_1539 at 1776.331 kb on - strand, within Pf1N1B4_1539 at 1776.628 kb on + strand, within Pf1N1B4_1539 at 1776.628 kb on + strand, within Pf1N1B4_1539 at 1776.628 kb on + strand, within Pf1N1B4_1539 at 1776.629 kb on - strand, within Pf1N1B4_1539 at 1776.629 kb on - strand, within Pf1N1B4_1539 at 1776.629 kb on - strand, within Pf1N1B4_1539 at 1776.629 kb on - strand, within Pf1N1B4_1539 at 1776.629 kb on - strand, within Pf1N1B4_1539 at 1776.894 kb on + strand at 1776.929 kb on + strand at 1776.930 kb on - strand at 1776.943 kb on - strand at 1776.957 kb on + strand at 1776.958 kb on - strand at 1777.100 kb on + strand, within Pf1N1B4_1540 at 1777.217 kb on + strand, within Pf1N1B4_1540 at 1777.217 kb on + strand, within Pf1N1B4_1540 at 1777.326 kb on + strand, within Pf1N1B4_1540 at 1777.328 kb on + strand, within Pf1N1B4_1540 at 1777.328 kb on + strand, within Pf1N1B4_1540 at 1777.328 kb on + strand, within Pf1N1B4_1540 at 1777.329 kb on - strand, within Pf1N1B4_1540 at 1777.329 kb on - strand, within Pf1N1B4_1540 at 1777.406 kb on + strand, within Pf1N1B4_1540 at 1777.519 kb on + strand at 1777.519 kb on + strand at 1777.519 kb on + strand at 1777.696 kb on + strand, within Pf1N1B4_1541 at 1777.696 kb on + strand, within Pf1N1B4_1541 at 1777.696 kb on + strand, within Pf1N1B4_1541 at 1777.697 kb on - strand at 1777.862 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.864 kb on + strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1777.865 kb on - strand, within Pf1N1B4_1541 at 1778.120 kb on - strand at 1778.341 kb on - strand, within Pf1N1B4_1542
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 1,776,217 + Pf1N1B4_1539 0.64 +1.6 1,776,217 + Pf1N1B4_1539 0.64 +1.0 1,776,217 + Pf1N1B4_1539 0.64 +0.3 1,776,218 - Pf1N1B4_1539 0.64 -0.5 1,776,226 + Pf1N1B4_1539 0.65 -0.2 1,776,330 + Pf1N1B4_1539 0.70 +0.2 1,776,331 - Pf1N1B4_1539 0.70 -0.7 1,776,628 + Pf1N1B4_1539 0.84 -0.2 1,776,628 + Pf1N1B4_1539 0.84 +0.1 1,776,628 + Pf1N1B4_1539 0.84 -0.2 1,776,629 - Pf1N1B4_1539 0.84 -1.2 1,776,629 - Pf1N1B4_1539 0.84 -0.6 1,776,629 - Pf1N1B4_1539 0.84 -0.7 1,776,629 - Pf1N1B4_1539 0.84 +0.6 1,776,629 - Pf1N1B4_1539 0.84 -0.3 1,776,894 + +0.4 1,776,929 + -0.9 1,776,930 - -0.1 1,776,943 - +0.3 1,776,957 + -0.4 1,776,958 - -0.1 1,777,100 + Pf1N1B4_1540 0.25 -1.1 1,777,217 + Pf1N1B4_1540 0.47 -0.5 1,777,217 + Pf1N1B4_1540 0.47 +0.8 1,777,326 + Pf1N1B4_1540 0.67 +0.0 1,777,328 + Pf1N1B4_1540 0.68 -0.8 1,777,328 + Pf1N1B4_1540 0.68 -0.9 1,777,328 + Pf1N1B4_1540 0.68 -2.8 1,777,329 - Pf1N1B4_1540 0.68 -2.4 1,777,329 - Pf1N1B4_1540 0.68 +0.3 1,777,406 + Pf1N1B4_1540 0.83 -0.0 1,777,519 + +0.8 1,777,519 + -0.2 1,777,519 + +0.9 1,777,696 + Pf1N1B4_1541 0.32 +0.9 1,777,696 + Pf1N1B4_1541 0.32 -1.1 1,777,696 + Pf1N1B4_1541 0.32 +0.4 1,777,697 - -0.4 1,777,862 + Pf1N1B4_1541 0.59 +1.3 1,777,864 + Pf1N1B4_1541 0.59 -0.9 1,777,864 + Pf1N1B4_1541 0.59 +0.2 1,777,864 + Pf1N1B4_1541 0.59 +0.6 1,777,864 + Pf1N1B4_1541 0.59 -1.0 1,777,864 + Pf1N1B4_1541 0.59 +0.2 1,777,864 + Pf1N1B4_1541 0.59 -0.0 1,777,864 + Pf1N1B4_1541 0.59 +0.1 1,777,864 + Pf1N1B4_1541 0.59 -0.0 1,777,864 + Pf1N1B4_1541 0.59 -0.4 1,777,864 + Pf1N1B4_1541 0.59 -0.3 1,777,864 + Pf1N1B4_1541 0.59 -0.1 1,777,864 + Pf1N1B4_1541 0.59 +0.2 1,777,864 + Pf1N1B4_1541 0.59 -2.4 1,777,864 + Pf1N1B4_1541 0.59 +0.9 1,777,864 + Pf1N1B4_1541 0.59 -0.1 1,777,865 - Pf1N1B4_1541 0.59 -0.7 1,777,865 - Pf1N1B4_1541 0.59 -0.5 1,777,865 - Pf1N1B4_1541 0.59 +0.2 1,777,865 - -0.4 1,777,865 - Pf1N1B4_1541 0.59 +1.5 1,777,865 - Pf1N1B4_1541 0.59 +0.3 1,777,865 - Pf1N1B4_1541 0.59 +1.2 1,777,865 - Pf1N1B4_1541 0.59 +0.1 1,777,865 - Pf1N1B4_1541 0.59 +0.1 1,778,120 - +0.0 1,778,341 - Pf1N1B4_1542 0.40 -0.1
Or see this region's nucleotide sequence