Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1540

Experiment: 4C survival; 24 hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1539 and Pf1N1B4_1540 are separated by 13 nucleotidesPf1N1B4_1540 and Pf1N1B4_1541 overlap by 4 nucleotidesPf1N1B4_1541 and Pf1N1B4_1542 overlap by 4 nucleotides Pf1N1B4_1539 - Type IV pilus biogenesis protein PilQ, at 1,774,881 to 1,776,956 _1539 Pf1N1B4_1540 - Type IV pilus biogenesis protein PilP, at 1,776,970 to 1,777,497 _1540 Pf1N1B4_1541 - Type IV pilus biogenesis protein PilO, at 1,777,494 to 1,778,117 _1541 Pf1N1B4_1542 - Type IV pilus biogenesis protein PilN, at 1,778,114 to 1,778,680 _1542 Position (kb) 1776 1777 1778Strain fitness (log2 ratio) -3 -2 -1 0 1at 1776.217 kb on + strand, within Pf1N1B4_1539at 1776.217 kb on + strand, within Pf1N1B4_1539at 1776.217 kb on + strand, within Pf1N1B4_1539at 1776.218 kb on - strand, within Pf1N1B4_1539at 1776.226 kb on + strand, within Pf1N1B4_1539at 1776.330 kb on + strand, within Pf1N1B4_1539at 1776.331 kb on - strand, within Pf1N1B4_1539at 1776.628 kb on + strand, within Pf1N1B4_1539at 1776.628 kb on + strand, within Pf1N1B4_1539at 1776.628 kb on + strand, within Pf1N1B4_1539at 1776.629 kb on - strand, within Pf1N1B4_1539at 1776.629 kb on - strand, within Pf1N1B4_1539at 1776.629 kb on - strand, within Pf1N1B4_1539at 1776.629 kb on - strand, within Pf1N1B4_1539at 1776.629 kb on - strand, within Pf1N1B4_1539at 1776.894 kb on + strandat 1776.929 kb on + strandat 1776.930 kb on - strandat 1776.943 kb on - strandat 1776.957 kb on + strandat 1776.958 kb on - strandat 1777.100 kb on + strand, within Pf1N1B4_1540at 1777.217 kb on + strand, within Pf1N1B4_1540at 1777.217 kb on + strand, within Pf1N1B4_1540at 1777.326 kb on + strand, within Pf1N1B4_1540at 1777.328 kb on + strand, within Pf1N1B4_1540at 1777.328 kb on + strand, within Pf1N1B4_1540at 1777.328 kb on + strand, within Pf1N1B4_1540at 1777.329 kb on - strand, within Pf1N1B4_1540at 1777.329 kb on - strand, within Pf1N1B4_1540at 1777.406 kb on + strand, within Pf1N1B4_1540at 1777.519 kb on + strandat 1777.519 kb on + strandat 1777.519 kb on + strandat 1777.696 kb on + strand, within Pf1N1B4_1541at 1777.696 kb on + strand, within Pf1N1B4_1541at 1777.696 kb on + strand, within Pf1N1B4_1541at 1777.697 kb on - strandat 1777.862 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.864 kb on + strand, within Pf1N1B4_1541at 1777.865 kb on - strand, within Pf1N1B4_1541at 1777.865 kb on - strand, within Pf1N1B4_1541at 1777.865 kb on - strand, within Pf1N1B4_1541at 1777.865 kb on - strandat 1777.865 kb on - strand, within Pf1N1B4_1541at 1777.865 kb on - strand, within Pf1N1B4_1541at 1777.865 kb on - strand, within Pf1N1B4_1541at 1777.865 kb on - strand, within Pf1N1B4_1541at 1777.865 kb on - strand, within Pf1N1B4_1541at 1778.120 kb on - strandat 1778.341 kb on - strand, within Pf1N1B4_1542

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Per-strain Table

Position Strand Gene LocusTag Fraction 4C survival; 24 hrs
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1,776,217 + Pf1N1B4_1539 0.64 +1.6
1,776,217 + Pf1N1B4_1539 0.64 +1.0
1,776,217 + Pf1N1B4_1539 0.64 +0.3
1,776,218 - Pf1N1B4_1539 0.64 -0.5
1,776,226 + Pf1N1B4_1539 0.65 -0.2
1,776,330 + Pf1N1B4_1539 0.70 +0.2
1,776,331 - Pf1N1B4_1539 0.70 -0.7
1,776,628 + Pf1N1B4_1539 0.84 -0.2
1,776,628 + Pf1N1B4_1539 0.84 +0.1
1,776,628 + Pf1N1B4_1539 0.84 -0.2
1,776,629 - Pf1N1B4_1539 0.84 -1.2
1,776,629 - Pf1N1B4_1539 0.84 -0.6
1,776,629 - Pf1N1B4_1539 0.84 -0.7
1,776,629 - Pf1N1B4_1539 0.84 +0.6
1,776,629 - Pf1N1B4_1539 0.84 -0.3
1,776,894 + +0.4
1,776,929 + -0.9
1,776,930 - -0.1
1,776,943 - +0.3
1,776,957 + -0.4
1,776,958 - -0.1
1,777,100 + Pf1N1B4_1540 0.25 -1.1
1,777,217 + Pf1N1B4_1540 0.47 -0.5
1,777,217 + Pf1N1B4_1540 0.47 +0.8
1,777,326 + Pf1N1B4_1540 0.67 +0.0
1,777,328 + Pf1N1B4_1540 0.68 -0.8
1,777,328 + Pf1N1B4_1540 0.68 -0.9
1,777,328 + Pf1N1B4_1540 0.68 -2.8
1,777,329 - Pf1N1B4_1540 0.68 -2.4
1,777,329 - Pf1N1B4_1540 0.68 +0.3
1,777,406 + Pf1N1B4_1540 0.83 -0.0
1,777,519 + +0.8
1,777,519 + -0.2
1,777,519 + +0.9
1,777,696 + Pf1N1B4_1541 0.32 +0.9
1,777,696 + Pf1N1B4_1541 0.32 -1.1
1,777,696 + Pf1N1B4_1541 0.32 +0.4
1,777,697 - -0.4
1,777,862 + Pf1N1B4_1541 0.59 +1.3
1,777,864 + Pf1N1B4_1541 0.59 -0.9
1,777,864 + Pf1N1B4_1541 0.59 +0.2
1,777,864 + Pf1N1B4_1541 0.59 +0.6
1,777,864 + Pf1N1B4_1541 0.59 -1.0
1,777,864 + Pf1N1B4_1541 0.59 +0.2
1,777,864 + Pf1N1B4_1541 0.59 -0.0
1,777,864 + Pf1N1B4_1541 0.59 +0.1
1,777,864 + Pf1N1B4_1541 0.59 -0.0
1,777,864 + Pf1N1B4_1541 0.59 -0.4
1,777,864 + Pf1N1B4_1541 0.59 -0.3
1,777,864 + Pf1N1B4_1541 0.59 -0.1
1,777,864 + Pf1N1B4_1541 0.59 +0.2
1,777,864 + Pf1N1B4_1541 0.59 -2.4
1,777,864 + Pf1N1B4_1541 0.59 +0.9
1,777,864 + Pf1N1B4_1541 0.59 -0.1
1,777,865 - Pf1N1B4_1541 0.59 -0.7
1,777,865 - Pf1N1B4_1541 0.59 -0.5
1,777,865 - Pf1N1B4_1541 0.59 +0.2
1,777,865 - -0.4
1,777,865 - Pf1N1B4_1541 0.59 +1.5
1,777,865 - Pf1N1B4_1541 0.59 +0.3
1,777,865 - Pf1N1B4_1541 0.59 +1.2
1,777,865 - Pf1N1B4_1541 0.59 +0.1
1,777,865 - Pf1N1B4_1541 0.59 +0.1
1,778,120 - +0.0
1,778,341 - Pf1N1B4_1542 0.40 -0.1

Or see this region's nucleotide sequence