Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1329 and Pf1N1B4_1330 are separated by 145 nucleotides Pf1N1B4_1330 and Pf1N1B4_1331 are separated by 242 nucleotides
Pf1N1B4_1329 - Cobalamin biosynthesis protein CobG, at 1,523,778 to 1,525,079
_1329
Pf1N1B4_1330 - hypothetical protein, at 1,525,225 to 1,525,881
_1330
Pf1N1B4_1331 - Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-), at 1,526,124 to 1,527,335
_1331
Position (kb)
1525
1526 Strain fitness (log2 ratio)
-2
-1
0
1 at 1524.283 kb on - strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.301 kb on + strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.758 kb on + strand, within Pf1N1B4_1329 at 1524.758 kb on + strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1525.065 kb on + strand at 1525.066 kb on - strand at 1525.145 kb on - strand at 1525.201 kb on - strand at 1525.201 kb on - strand at 1525.208 kb on - strand at 1525.236 kb on - strand at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.383 kb on + strand, within Pf1N1B4_1330 at 1525.384 kb on - strand, within Pf1N1B4_1330 at 1525.448 kb on + strand, within Pf1N1B4_1330 at 1525.464 kb on + strand, within Pf1N1B4_1330 at 1525.729 kb on - strand, within Pf1N1B4_1330 at 1525.731 kb on - strand, within Pf1N1B4_1330 at 1525.731 kb on - strand, within Pf1N1B4_1330 at 1525.759 kb on + strand, within Pf1N1B4_1330 at 1525.825 kb on - strand at 1525.825 kb on - strand at 1525.825 kb on - strand at 1525.882 kb on - strand at 1525.975 kb on + strand at 1526.016 kb on + strand at 1526.016 kb on + strand at 1526.339 kb on - strand, within Pf1N1B4_1331 at 1526.482 kb on + strand, within Pf1N1B4_1331 at 1526.483 kb on - strand, within Pf1N1B4_1331
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 1,524,283 - Pf1N1B4_1329 0.39 +0.6 1,524,295 + Pf1N1B4_1329 0.40 -0.8 1,524,295 + Pf1N1B4_1329 0.40 -0.5 1,524,295 + -0.7 1,524,295 + Pf1N1B4_1329 0.40 -0.1 1,524,295 + Pf1N1B4_1329 0.40 +0.2 1,524,295 + Pf1N1B4_1329 0.40 +0.4 1,524,295 + Pf1N1B4_1329 0.40 -1.7 1,524,296 - Pf1N1B4_1329 0.40 +0.1 1,524,296 - Pf1N1B4_1329 0.40 +0.1 1,524,296 - Pf1N1B4_1329 0.40 +1.4 1,524,296 - Pf1N1B4_1329 0.40 +0.5 1,524,296 - Pf1N1B4_1329 0.40 +0.8 1,524,296 - Pf1N1B4_1329 0.40 -0.8 1,524,296 - Pf1N1B4_1329 0.40 -0.6 1,524,296 - Pf1N1B4_1329 0.40 -1.0 1,524,296 - Pf1N1B4_1329 0.40 -0.2 1,524,296 - Pf1N1B4_1329 0.40 +0.4 1,524,296 - Pf1N1B4_1329 0.40 +0.2 1,524,296 - Pf1N1B4_1329 0.40 -0.2 1,524,296 - Pf1N1B4_1329 0.40 -0.8 1,524,301 + Pf1N1B4_1329 0.40 -0.0 1,524,302 - Pf1N1B4_1329 0.40 +0.9 1,524,302 - Pf1N1B4_1329 0.40 +0.5 1,524,302 - Pf1N1B4_1329 0.40 +0.3 1,524,302 - Pf1N1B4_1329 0.40 -1.8 1,524,302 - Pf1N1B4_1329 0.40 +0.6 1,524,758 + Pf1N1B4_1329 0.75 -1.8 1,524,758 + Pf1N1B4_1329 0.75 -0.4 1,524,759 - Pf1N1B4_1329 0.75 -0.8 1,524,759 - Pf1N1B4_1329 0.75 +0.8 1,524,759 - Pf1N1B4_1329 0.75 +0.8 1,524,759 - Pf1N1B4_1329 0.75 -0.2 1,525,065 + -0.6 1,525,066 - +0.2 1,525,145 - -0.7 1,525,201 - -0.7 1,525,201 - +0.2 1,525,208 - +0.1 1,525,236 - -0.5 1,525,377 - Pf1N1B4_1330 0.23 -0.7 1,525,377 - Pf1N1B4_1330 0.23 -0.1 1,525,377 - Pf1N1B4_1330 0.23 -0.2 1,525,383 + Pf1N1B4_1330 0.24 -0.1 1,525,384 - Pf1N1B4_1330 0.24 -0.8 1,525,448 + Pf1N1B4_1330 0.34 -0.4 1,525,464 + Pf1N1B4_1330 0.36 -1.6 1,525,729 - Pf1N1B4_1330 0.77 -1.5 1,525,731 - Pf1N1B4_1330 0.77 +0.0 1,525,731 - Pf1N1B4_1330 0.77 -1.6 1,525,759 + Pf1N1B4_1330 0.81 +0.0 1,525,825 - +0.4 1,525,825 - -0.5 1,525,825 - -0.5 1,525,882 - -0.3 1,525,975 + +0.4 1,526,016 + -0.1 1,526,016 + -0.7 1,526,339 - Pf1N1B4_1331 0.18 -0.0 1,526,482 + Pf1N1B4_1331 0.30 -0.9 1,526,483 - Pf1N1B4_1331 0.30 -0.7
Or see this region's nucleotide sequence