Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1113 and Pf1N1B4_1114 are separated by 63 nucleotides Pf1N1B4_1114 and Pf1N1B4_1115 are separated by 96 nucleotides Pf1N1B4_1115 and Pf1N1B4_1116 overlap by 8 nucleotides
Pf1N1B4_1113 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 1,247,309 to 1,248,184
_1113
Pf1N1B4_1114 - ABC transporter, ATP-binding protein, at 1,248,248 to 1,249,975
_1114
Pf1N1B4_1115 - Dna binding response regulator PrrA (RegA), at 1,250,072 to 1,250,632
_1115
Pf1N1B4_1116 - Sensor histidine kinase PrrB (RegB) (EC 2.7.3.-), at 1,250,625 to 1,251,887
_1116
Position (kb)
1248
1249
1250 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1247.428 kb on - strand, within Pf1N1B4_1113 at 1247.428 kb on - strand, within Pf1N1B4_1113 at 1247.428 kb on - strand, within Pf1N1B4_1113 at 1247.428 kb on - strand, within Pf1N1B4_1113 at 1247.463 kb on + strand, within Pf1N1B4_1113 at 1247.463 kb on + strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.464 kb on - strand, within Pf1N1B4_1113 at 1247.557 kb on - strand, within Pf1N1B4_1113 at 1247.557 kb on - strand, within Pf1N1B4_1113 at 1248.099 kb on + strand at 1248.099 kb on + strand at 1248.099 kb on + strand at 1248.100 kb on - strand at 1248.100 kb on - strand at 1248.115 kb on + strand at 1248.116 kb on - strand at 1248.116 kb on - strand at 1248.432 kb on + strand, within Pf1N1B4_1114 at 1248.433 kb on - strand, within Pf1N1B4_1114 at 1248.433 kb on - strand, within Pf1N1B4_1114 at 1248.433 kb on - strand, within Pf1N1B4_1114 at 1248.450 kb on + strand, within Pf1N1B4_1114 at 1248.451 kb on - strand, within Pf1N1B4_1114 at 1248.577 kb on - strand, within Pf1N1B4_1114 at 1248.634 kb on + strand, within Pf1N1B4_1114 at 1249.236 kb on + strand, within Pf1N1B4_1114 at 1249.272 kb on - strand, within Pf1N1B4_1114 at 1249.398 kb on + strand, within Pf1N1B4_1114 at 1249.570 kb on - strand, within Pf1N1B4_1114 at 1249.594 kb on - strand, within Pf1N1B4_1114 at 1249.594 kb on - strand, within Pf1N1B4_1114 at 1249.725 kb on + strand, within Pf1N1B4_1114 at 1249.777 kb on - strand, within Pf1N1B4_1114 at 1249.777 kb on - strand, within Pf1N1B4_1114 at 1249.957 kb on - strand at 1249.989 kb on + strand at 1249.990 kb on - strand at 1250.018 kb on + strand at 1250.019 kb on - strand at 1250.019 kb on - strand at 1250.174 kb on + strand, within Pf1N1B4_1115 at 1250.175 kb on - strand, within Pf1N1B4_1115 at 1250.175 kb on - strand, within Pf1N1B4_1115 at 1250.175 kb on - strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.304 kb on + strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.305 kb on - strand, within Pf1N1B4_1115 at 1250.457 kb on + strand, within Pf1N1B4_1115 at 1250.457 kb on + strand, within Pf1N1B4_1115 at 1250.458 kb on - strand, within Pf1N1B4_1115 at 1250.458 kb on - strand, within Pf1N1B4_1115 at 1250.465 kb on + strand, within Pf1N1B4_1115 at 1250.465 kb on + strand, within Pf1N1B4_1115 at 1250.465 kb on + strand, within Pf1N1B4_1115 at 1250.465 kb on + strand, within Pf1N1B4_1115 at 1250.465 kb on + strand, within Pf1N1B4_1115 at 1250.466 kb on - strand, within Pf1N1B4_1115 at 1250.466 kb on - strand at 1250.466 kb on - strand, within Pf1N1B4_1115 at 1250.468 kb on - strand, within Pf1N1B4_1115 at 1250.512 kb on + strand, within Pf1N1B4_1115 at 1250.513 kb on - strand, within Pf1N1B4_1115 at 1250.579 kb on + strand at 1250.579 kb on + strand at 1250.580 kb on - strand at 1250.580 kb on - strand at 1250.580 kb on - strand at 1250.698 kb on + strand at 1250.698 kb on + strand at 1250.698 kb on + strand at 1250.698 kb on + strand at 1250.698 kb on + strand at 1250.698 kb on + strand at 1250.698 kb on + strand at 1250.698 kb on + strand at 1250.699 kb on - strand at 1250.699 kb on - strand at 1250.699 kb on - strand at 1250.699 kb on - strand at 1250.699 kb on - strand at 1250.780 kb on + strand, within Pf1N1B4_1116 at 1250.780 kb on + strand, within Pf1N1B4_1116 at 1250.781 kb on - strand, within Pf1N1B4_1116 at 1250.781 kb on - strand, within Pf1N1B4_1116
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 1,247,428 - Pf1N1B4_1113 0.14 -0.4 1,247,428 - Pf1N1B4_1113 0.14 +0.2 1,247,428 - Pf1N1B4_1113 0.14 -0.1 1,247,428 - Pf1N1B4_1113 0.14 -0.3 1,247,463 + Pf1N1B4_1113 0.18 -0.3 1,247,463 + Pf1N1B4_1113 0.18 +0.5 1,247,464 - Pf1N1B4_1113 0.18 +0.6 1,247,464 - Pf1N1B4_1113 0.18 -0.2 1,247,464 - Pf1N1B4_1113 0.18 -0.8 1,247,464 - Pf1N1B4_1113 0.18 -0.6 1,247,464 - Pf1N1B4_1113 0.18 -0.9 1,247,464 - Pf1N1B4_1113 0.18 +0.1 1,247,464 - Pf1N1B4_1113 0.18 -0.6 1,247,464 - Pf1N1B4_1113 0.18 +0.1 1,247,464 - Pf1N1B4_1113 0.18 -0.0 1,247,464 - Pf1N1B4_1113 0.18 -1.3 1,247,464 - Pf1N1B4_1113 0.18 +0.1 1,247,464 - Pf1N1B4_1113 0.18 +0.1 1,247,557 - Pf1N1B4_1113 0.28 -1.9 1,247,557 - Pf1N1B4_1113 0.28 -0.5 1,248,099 + -1.0 1,248,099 + +1.2 1,248,099 + +0.4 1,248,100 - +0.5 1,248,100 - -1.4 1,248,115 + +0.4 1,248,116 - -0.5 1,248,116 - +0.7 1,248,432 + Pf1N1B4_1114 0.11 -0.1 1,248,433 - Pf1N1B4_1114 0.11 +0.1 1,248,433 - Pf1N1B4_1114 0.11 +0.7 1,248,433 - Pf1N1B4_1114 0.11 -0.9 1,248,450 + Pf1N1B4_1114 0.12 -0.6 1,248,451 - Pf1N1B4_1114 0.12 -1.6 1,248,577 - Pf1N1B4_1114 0.19 +0.5 1,248,634 + Pf1N1B4_1114 0.22 -0.5 1,249,236 + Pf1N1B4_1114 0.57 -0.1 1,249,272 - Pf1N1B4_1114 0.59 -1.1 1,249,398 + Pf1N1B4_1114 0.67 -2.3 1,249,570 - Pf1N1B4_1114 0.77 -0.7 1,249,594 - Pf1N1B4_1114 0.78 -0.9 1,249,594 - Pf1N1B4_1114 0.78 -0.6 1,249,725 + Pf1N1B4_1114 0.85 -0.1 1,249,777 - Pf1N1B4_1114 0.88 -1.0 1,249,777 - Pf1N1B4_1114 0.88 -0.4 1,249,957 - -0.1 1,249,989 + -0.5 1,249,990 - +1.1 1,250,018 + +0.2 1,250,019 - -0.2 1,250,019 - -0.1 1,250,174 + Pf1N1B4_1115 0.18 -2.8 1,250,175 - Pf1N1B4_1115 0.18 -0.5 1,250,175 - Pf1N1B4_1115 0.18 -0.6 1,250,175 - Pf1N1B4_1115 0.18 -0.4 1,250,304 + Pf1N1B4_1115 0.41 +0.5 1,250,304 + Pf1N1B4_1115 0.41 -1.1 1,250,304 + Pf1N1B4_1115 0.41 -0.2 1,250,304 + Pf1N1B4_1115 0.41 -1.7 1,250,304 + Pf1N1B4_1115 0.41 +0.7 1,250,304 + Pf1N1B4_1115 0.41 -1.8 1,250,304 + Pf1N1B4_1115 0.41 -1.6 1,250,304 + Pf1N1B4_1115 0.41 +0.3 1,250,304 + Pf1N1B4_1115 0.41 -1.2 1,250,304 + Pf1N1B4_1115 0.41 -0.9 1,250,304 + Pf1N1B4_1115 0.41 -0.7 1,250,304 + Pf1N1B4_1115 0.41 -0.9 1,250,304 + Pf1N1B4_1115 0.41 -0.3 1,250,304 + Pf1N1B4_1115 0.41 +0.2 1,250,304 + Pf1N1B4_1115 0.41 -0.8 1,250,304 + Pf1N1B4_1115 0.41 -0.8 1,250,304 + Pf1N1B4_1115 0.41 -0.7 1,250,304 + Pf1N1B4_1115 0.41 -0.4 1,250,304 + Pf1N1B4_1115 0.41 +0.1 1,250,304 + Pf1N1B4_1115 0.41 -1.1 1,250,304 + Pf1N1B4_1115 0.41 -1.3 1,250,304 + Pf1N1B4_1115 0.41 -0.4 1,250,305 - Pf1N1B4_1115 0.42 -1.1 1,250,305 - Pf1N1B4_1115 0.42 -0.3 1,250,305 - Pf1N1B4_1115 0.42 -1.1 1,250,305 - Pf1N1B4_1115 0.42 -0.8 1,250,305 - Pf1N1B4_1115 0.42 -0.2 1,250,305 - Pf1N1B4_1115 0.42 -0.8 1,250,305 - +0.5 1,250,305 - Pf1N1B4_1115 0.42 -0.9 1,250,305 - Pf1N1B4_1115 0.42 -1.4 1,250,305 - Pf1N1B4_1115 0.42 -0.1 1,250,305 - Pf1N1B4_1115 0.42 -1.5 1,250,305 - Pf1N1B4_1115 0.42 -0.8 1,250,305 - Pf1N1B4_1115 0.42 -1.0 1,250,305 - Pf1N1B4_1115 0.42 -0.6 1,250,305 - Pf1N1B4_1115 0.42 -0.4 1,250,305 - Pf1N1B4_1115 0.42 -0.9 1,250,305 - Pf1N1B4_1115 0.42 -1.7 1,250,305 - Pf1N1B4_1115 0.42 -0.5 1,250,305 - Pf1N1B4_1115 0.42 +0.8 1,250,305 - Pf1N1B4_1115 0.42 -0.8 1,250,457 + Pf1N1B4_1115 0.69 -0.6 1,250,457 + Pf1N1B4_1115 0.69 -1.1 1,250,458 - Pf1N1B4_1115 0.69 -0.9 1,250,458 - Pf1N1B4_1115 0.69 -0.7 1,250,465 + Pf1N1B4_1115 0.70 -0.3 1,250,465 + Pf1N1B4_1115 0.70 -1.4 1,250,465 + Pf1N1B4_1115 0.70 -0.1 1,250,465 + Pf1N1B4_1115 0.70 -0.6 1,250,465 + Pf1N1B4_1115 0.70 -1.2 1,250,466 - Pf1N1B4_1115 0.70 -0.9 1,250,466 - -0.6 1,250,466 - Pf1N1B4_1115 0.70 -1.3 1,250,468 - Pf1N1B4_1115 0.71 +0.1 1,250,512 + Pf1N1B4_1115 0.78 -1.1 1,250,513 - Pf1N1B4_1115 0.79 -1.4 1,250,579 + -2.6 1,250,579 + -0.6 1,250,580 - -1.8 1,250,580 - -0.9 1,250,580 - -1.2 1,250,698 + -1.1 1,250,698 + -0.1 1,250,698 + -1.9 1,250,698 + +0.4 1,250,698 + -0.9 1,250,698 + -0.1 1,250,698 + -0.4 1,250,698 + -0.8 1,250,699 - -1.1 1,250,699 - -1.9 1,250,699 - -0.4 1,250,699 - -0.5 1,250,699 - -0.6 1,250,780 + Pf1N1B4_1116 0.12 -0.7 1,250,780 + Pf1N1B4_1116 0.12 +0.3 1,250,781 - Pf1N1B4_1116 0.12 -0.5 1,250,781 - Pf1N1B4_1116 0.12 -0.7
Or see this region's nucleotide sequence