Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_105 and Pf1N1B4_106 are separated by 3 nucleotides Pf1N1B4_106 and Pf1N1B4_107 are separated by 38 nucleotides Pf1N1B4_107 and Pf1N1B4_108 are separated by 243 nucleotides Pf1N1B4_108 and Pf1N1B4_109 are separated by 82 nucleotides Pf1N1B4_109 and Pf1N1B4_110 are separated by 17 nucleotides Pf1N1B4_110 and Pf1N1B4_111 are separated by 226 nucleotides
Pf1N1B4_105 - Two-component system response regulator QseB, at 111,675 to 112,343
_105
Pf1N1B4_106 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 112,347 to 112,628
_106
Pf1N1B4_107 - FIG00857858: hypothetical protein, at 112,667 to 112,975
_107
Pf1N1B4_108 - FIG00954456: hypothetical protein, at 113,219 to 114,298
_108
Pf1N1B4_109 - hypothetical protein, at 114,381 to 114,536
_109
Pf1N1B4_110 - Queuosine biosynthesis QueD, PTPS-I, at 114,554 to 114,910
_110
Pf1N1B4_111 - Possible oxidoreductase, at 115,137 to 115,961
_111
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-2
-1
0
1 at 112.232 kb on - strand, within Pf1N1B4_105 at 112.364 kb on + strand at 112.364 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.369 kb on - strand at 112.395 kb on + strand, within Pf1N1B4_106 at 112.409 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand at 112.438 kb on + strand, within Pf1N1B4_106 at 112.444 kb on + strand, within Pf1N1B4_106 at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.761 kb on + strand, within Pf1N1B4_107 at 112.762 kb on - strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.777 kb on - strand at 112.864 kb on + strand, within Pf1N1B4_107 at 112.865 kb on - strand, within Pf1N1B4_107 at 112.988 kb on + strand at 113.006 kb on + strand at 113.006 kb on + strand at 113.007 kb on - strand at 113.007 kb on - strand at 113.016 kb on - strand at 113.016 kb on - strand at 113.016 kb on - strand at 113.020 kb on + strand at 113.020 kb on + strand at 113.035 kb on - strand at 113.087 kb on - strand at 113.126 kb on + strand at 113.127 kb on - strand at 113.128 kb on + strand at 113.154 kb on + strand at 113.154 kb on + strand at 113.155 kb on - strand at 113.510 kb on + strand, within Pf1N1B4_108 at 113.510 kb on + strand, within Pf1N1B4_108 at 113.961 kb on - strand, within Pf1N1B4_108 at 113.979 kb on - strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.200 kb on + strand at 114.201 kb on - strand at 114.234 kb on + strand at 114.315 kb on + strand at 114.316 kb on - strand at 114.494 kb on - strand, within Pf1N1B4_109 at 114.740 kb on + strand, within Pf1N1B4_110 at 114.740 kb on + strand, within Pf1N1B4_110 at 114.741 kb on - strand, within Pf1N1B4_110 at 114.757 kb on + strand, within Pf1N1B4_110 at 114.758 kb on - strand at 114.758 kb on - strand, within Pf1N1B4_110 at 114.758 kb on - strand, within Pf1N1B4_110 at 114.758 kb on - strand, within Pf1N1B4_110 at 114.758 kb on - strand, within Pf1N1B4_110 at 114.761 kb on + strand, within Pf1N1B4_110 at 114.761 kb on + strand, within Pf1N1B4_110 at 114.762 kb on - strand, within Pf1N1B4_110 at 114.762 kb on - strand, within Pf1N1B4_110 at 114.762 kb on - strand at 114.896 kb on + strand at 115.064 kb on - strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.100 kb on - strand at 115.100 kb on - strand at 115.100 kb on - strand at 115.165 kb on - strand at 115.198 kb on + strand at 115.251 kb on + strand, within Pf1N1B4_111 at 115.251 kb on + strand, within Pf1N1B4_111 at 115.251 kb on + strand, within Pf1N1B4_111 at 115.251 kb on + strand, within Pf1N1B4_111 at 115.252 kb on - strand, within Pf1N1B4_111 at 115.252 kb on - strand, within Pf1N1B4_111 at 115.252 kb on - strand, within Pf1N1B4_111 at 115.255 kb on + strand, within Pf1N1B4_111 at 115.255 kb on + strand at 115.255 kb on + strand, within Pf1N1B4_111 at 115.255 kb on + strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 112,232 - Pf1N1B4_105 0.83 +0.6 112,364 + -0.9 112,364 + -0.8 112,368 + -1.1 112,368 + -0.1 112,368 + -0.5 112,369 - -2.6 112,395 + Pf1N1B4_106 0.17 -0.4 112,409 + Pf1N1B4_106 0.22 -1.2 112,438 + Pf1N1B4_106 0.32 -0.3 112,438 + -1.7 112,438 + Pf1N1B4_106 0.32 -0.3 112,444 + Pf1N1B4_106 0.34 -1.1 112,668 + +0.9 112,668 + +0.8 112,668 + +0.6 112,668 + -0.6 112,668 + +0.1 112,761 + Pf1N1B4_107 0.30 +0.2 112,762 - Pf1N1B4_107 0.31 -2.2 112,767 + Pf1N1B4_107 0.32 -1.1 112,767 + Pf1N1B4_107 0.32 +1.2 112,767 + Pf1N1B4_107 0.32 -1.0 112,768 - Pf1N1B4_107 0.33 +0.2 112,768 - Pf1N1B4_107 0.33 +0.1 112,768 - Pf1N1B4_107 0.33 -0.2 112,768 - Pf1N1B4_107 0.33 -0.3 112,777 - +0.5 112,864 + Pf1N1B4_107 0.64 +0.6 112,865 - Pf1N1B4_107 0.64 -0.1 112,988 + +1.3 113,006 + -1.1 113,006 + +0.9 113,007 - +0.6 113,007 - -0.7 113,016 - -1.0 113,016 - +0.6 113,016 - +0.4 113,020 + -0.8 113,020 + -0.4 113,035 - -0.5 113,087 - +0.2 113,126 + -0.0 113,127 - +0.6 113,128 + -1.0 113,154 + +0.2 113,154 + -0.6 113,155 - +0.6 113,510 + Pf1N1B4_108 0.27 -0.6 113,510 + Pf1N1B4_108 0.27 +0.3 113,961 - Pf1N1B4_108 0.69 -1.2 113,979 - Pf1N1B4_108 0.70 +0.1 114,116 + Pf1N1B4_108 0.83 -1.3 114,116 + Pf1N1B4_108 0.83 -0.6 114,116 + Pf1N1B4_108 0.83 -1.1 114,116 + Pf1N1B4_108 0.83 -0.4 114,200 + -1.3 114,201 - -0.0 114,234 + +1.2 114,315 + +0.1 114,316 - -1.9 114,494 - Pf1N1B4_109 0.72 -1.2 114,740 + Pf1N1B4_110 0.52 -1.8 114,740 + Pf1N1B4_110 0.52 +0.6 114,741 - Pf1N1B4_110 0.52 +0.8 114,757 + Pf1N1B4_110 0.57 -0.7 114,758 - +0.2 114,758 - Pf1N1B4_110 0.57 -1.1 114,758 - Pf1N1B4_110 0.57 -0.3 114,758 - Pf1N1B4_110 0.57 -0.4 114,758 - Pf1N1B4_110 0.57 -1.4 114,761 + Pf1N1B4_110 0.58 +1.2 114,761 + Pf1N1B4_110 0.58 -0.0 114,762 - Pf1N1B4_110 0.58 -0.1 114,762 - Pf1N1B4_110 0.58 -2.1 114,762 - -0.7 114,896 + +1.3 115,064 - -0.3 115,099 + -1.4 115,099 + -0.3 115,099 + +0.2 115,099 + -0.3 115,099 + -0.7 115,099 + +0.3 115,100 - -0.1 115,100 - -0.2 115,100 - +1.3 115,165 - -0.9 115,198 + -0.9 115,251 + Pf1N1B4_111 0.14 +0.2 115,251 + Pf1N1B4_111 0.14 -0.2 115,251 + Pf1N1B4_111 0.14 +1.3 115,251 + Pf1N1B4_111 0.14 +0.1 115,252 - Pf1N1B4_111 0.14 -0.5 115,252 - Pf1N1B4_111 0.14 -1.2 115,252 - Pf1N1B4_111 0.14 -0.5 115,255 + Pf1N1B4_111 0.14 +0.5 115,255 + -0.5 115,255 + Pf1N1B4_111 0.14 -1.1 115,255 + Pf1N1B4_111 0.14 -0.0 115,256 - Pf1N1B4_111 0.14 -0.3 115,256 - Pf1N1B4_111 0.14 +1.0 115,256 - Pf1N1B4_111 0.14 -0.5 115,256 - Pf1N1B4_111 0.14 +0.7 115,256 - Pf1N1B4_111 0.14 -0.5
Or see this region's nucleotide sequence