Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25245

Experiment: L-Sorbose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25240 and BWI76_RS25245 are separated by 14 nucleotidesBWI76_RS25245 and BWI76_RS25250 overlap by 4 nucleotidesBWI76_RS25250 and BWI76_RS25255 overlap by 32 nucleotides BWI76_RS25240: BWI76_RS25240 - D-arabinose 5-phosphate isomerase, at 5,248,414 to 5,249,400 _RS25240 BWI76_RS25245: BWI76_RS25245 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 5,249,415 to 5,249,981 _RS25245 BWI76_RS25250: BWI76_RS25250 - LPS export ABC transporter periplasmic protein LptC, at 5,249,978 to 5,250,553 _RS25250 BWI76_RS25255: BWI76_RS25255 - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 5,250,522 to 5,251,067 _RS25255 Position (kb) 5249 5250Strain fitness (log2 ratio) -2 -1 0 1 2at 5248.489 kb on + strandat 5248.489 kb on + strandat 5248.694 kb on - strand, within BWI76_RS25240at 5248.730 kb on - strand, within BWI76_RS25240at 5248.858 kb on - strand, within BWI76_RS25240at 5248.882 kb on + strand, within BWI76_RS25240at 5248.907 kb on - strand, within BWI76_RS25240at 5248.917 kb on + strand, within BWI76_RS25240at 5249.215 kb on - strand, within BWI76_RS25240at 5249.395 kb on + strandat 5249.446 kb on + strandat 5249.595 kb on + strand, within BWI76_RS25245at 5249.720 kb on + strand, within BWI76_RS25245at 5249.823 kb on + strand, within BWI76_RS25245at 5249.945 kb on + strandat 5250.508 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Sorbose (C)
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5,248,489 + -0.7
5,248,489 + +0.4
5,248,694 - BWI76_RS25240 0.28 +0.7
5,248,730 - BWI76_RS25240 0.32 -0.2
5,248,858 - BWI76_RS25240 0.45 +0.7
5,248,882 + BWI76_RS25240 0.47 -2.1
5,248,907 - BWI76_RS25240 0.50 +2.3
5,248,917 + BWI76_RS25240 0.51 +0.3
5,249,215 - BWI76_RS25240 0.81 +0.3
5,249,395 + +0.3
5,249,446 + -0.1
5,249,595 + BWI76_RS25245 0.32 -0.1
5,249,720 + BWI76_RS25245 0.54 +0.7
5,249,823 + BWI76_RS25245 0.72 +1.8
5,249,945 + -0.3
5,250,508 + -1.1

Or see this region's nucleotide sequence