Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23935

Experiment: L-Sorbose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23930 and BWI76_RS23935 are separated by 198 nucleotidesBWI76_RS23935 and BWI76_RS23940 overlap by 8 nucleotidesBWI76_RS23940 and BWI76_RS23945 are separated by 10 nucleotides BWI76_RS23930: BWI76_RS23930 - transcriptional regulator ArgP, at 4,993,855 to 4,994,748 _RS23930 BWI76_RS23935: BWI76_RS23935 - methylmalonyl-CoA mutase, at 4,994,947 to 4,997,091 _RS23935 BWI76_RS23940: BWI76_RS23940 - ATPase/protein kinase, at 4,997,084 to 4,998,079 _RS23940 BWI76_RS23945: BWI76_RS23945 - methylmalonyl-CoA decarboxylase, at 4,998,090 to 4,998,875 _RS23945 Position (kb) 4994 4995 4996 4997 4998Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4994.015 kb on + strand, within BWI76_RS23930at 4994.269 kb on + strand, within BWI76_RS23930at 4994.288 kb on + strand, within BWI76_RS23930at 4994.357 kb on + strand, within BWI76_RS23930at 4994.402 kb on + strand, within BWI76_RS23930at 4994.750 kb on - strandat 4995.167 kb on - strand, within BWI76_RS23935at 4995.167 kb on - strand, within BWI76_RS23935at 4995.167 kb on - strand, within BWI76_RS23935at 4995.167 kb on - strand, within BWI76_RS23935at 4995.212 kb on - strand, within BWI76_RS23935at 4995.259 kb on + strand, within BWI76_RS23935at 4995.419 kb on + strand, within BWI76_RS23935at 4995.476 kb on + strand, within BWI76_RS23935at 4995.525 kb on + strand, within BWI76_RS23935at 4995.631 kb on - strand, within BWI76_RS23935at 4995.668 kb on - strand, within BWI76_RS23935at 4995.763 kb on - strand, within BWI76_RS23935at 4995.804 kb on - strand, within BWI76_RS23935at 4996.117 kb on - strand, within BWI76_RS23935at 4996.176 kb on + strand, within BWI76_RS23935at 4996.298 kb on - strand, within BWI76_RS23935at 4996.367 kb on + strand, within BWI76_RS23935at 4996.375 kb on - strand, within BWI76_RS23935at 4996.454 kb on + strand, within BWI76_RS23935at 4996.528 kb on - strand, within BWI76_RS23935at 4996.587 kb on + strand, within BWI76_RS23935at 4996.781 kb on + strand, within BWI76_RS23935at 4996.795 kb on - strand, within BWI76_RS23935at 4996.909 kb on - strandat 4997.038 kb on + strandat 4997.148 kb on - strandat 4997.426 kb on + strand, within BWI76_RS23940at 4997.519 kb on + strand, within BWI76_RS23940at 4997.519 kb on + strand, within BWI76_RS23940at 4997.701 kb on - strand, within BWI76_RS23940at 4997.701 kb on - strand, within BWI76_RS23940at 4997.947 kb on + strand, within BWI76_RS23940

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Sorbose (C)
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4,994,015 + BWI76_RS23930 0.18 -2.5
4,994,269 + BWI76_RS23930 0.46 -1.2
4,994,288 + BWI76_RS23930 0.48 -1.8
4,994,357 + BWI76_RS23930 0.56 -0.7
4,994,402 + BWI76_RS23930 0.61 -0.9
4,994,750 - -2.5
4,995,167 - BWI76_RS23935 0.10 -0.2
4,995,167 - BWI76_RS23935 0.10 +0.2
4,995,167 - BWI76_RS23935 0.10 +0.4
4,995,167 - BWI76_RS23935 0.10 +0.0
4,995,212 - BWI76_RS23935 0.12 -0.3
4,995,259 + BWI76_RS23935 0.15 -0.5
4,995,419 + BWI76_RS23935 0.22 +0.8
4,995,476 + BWI76_RS23935 0.25 +0.6
4,995,525 + BWI76_RS23935 0.27 +3.2
4,995,631 - BWI76_RS23935 0.32 -0.1
4,995,668 - BWI76_RS23935 0.34 -0.4
4,995,763 - BWI76_RS23935 0.38 +0.1
4,995,804 - BWI76_RS23935 0.40 +1.1
4,996,117 - BWI76_RS23935 0.55 -0.6
4,996,176 + BWI76_RS23935 0.57 +0.9
4,996,298 - BWI76_RS23935 0.63 +0.8
4,996,367 + BWI76_RS23935 0.66 -0.3
4,996,375 - BWI76_RS23935 0.67 +0.7
4,996,454 + BWI76_RS23935 0.70 -0.7
4,996,528 - BWI76_RS23935 0.74 -0.3
4,996,587 + BWI76_RS23935 0.76 -0.1
4,996,781 + BWI76_RS23935 0.86 -2.1
4,996,795 - BWI76_RS23935 0.86 +0.5
4,996,909 - +1.5
4,997,038 + -1.2
4,997,148 - -0.6
4,997,426 + BWI76_RS23940 0.34 +0.0
4,997,519 + BWI76_RS23940 0.44 +0.3
4,997,519 + BWI76_RS23940 0.44 +0.4
4,997,701 - BWI76_RS23940 0.62 -0.2
4,997,701 - BWI76_RS23940 0.62 -1.1
4,997,947 + BWI76_RS23940 0.87 +1.1

Or see this region's nucleotide sequence