Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20655

Experiment: L-Sorbose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20650 and BWI76_RS20655 are separated by 42 nucleotidesBWI76_RS20655 and BWI76_RS20660 are separated by 42 nucleotidesBWI76_RS20660 and BWI76_RS20665 overlap by 4 nucleotides BWI76_RS20650: BWI76_RS20650 - phosphoenolpyruvate--protein phosphotransferase, at 4,320,863 to 4,322,590 _RS20650 BWI76_RS20655: BWI76_RS20655 - glucose-specific phosphotransferase enzyme IIA component, at 4,322,633 to 4,323,142 _RS20655 BWI76_RS20660: BWI76_RS20660 - hypothetical protein, at 4,323,185 to 4,323,424 _RS20660 BWI76_RS20665: BWI76_RS20665 - bifunctional pyridoxal kinase/hydroxymethylpyrimidine kinase, at 4,323,421 to 4,324,287 _RS20665 Position (kb) 4322 4323 4324Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4321.684 kb on + strand, within BWI76_RS20650at 4322.527 kb on + strandat 4323.795 kb on + strand, within BWI76_RS20665at 4323.959 kb on + strand, within BWI76_RS20665at 4323.967 kb on - strand, within BWI76_RS20665at 4324.019 kb on + strand, within BWI76_RS20665at 4324.114 kb on - strand, within BWI76_RS20665

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Sorbose (C)
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4,321,684 + BWI76_RS20650 0.48 -3.8
4,322,527 + -1.8
4,323,795 + BWI76_RS20665 0.43 -0.7
4,323,959 + BWI76_RS20665 0.62 +0.1
4,323,967 - BWI76_RS20665 0.63 +1.7
4,324,019 + BWI76_RS20665 0.69 +0.2
4,324,114 - BWI76_RS20665 0.80 -0.3

Or see this region's nucleotide sequence