Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_06380 and IAI47_06385 are separated by 109 nucleotides IAI47_06385 and IAI47_06390 overlap by 4 nucleotides IAI47_06390 and IAI47_06395 are separated by 11 nucleotides
IAI47_06380: IAI47_06380 - NADH-quinone oxidoreductase subunit NuoF, at 1,297,419 to 1,298,759
_06380
IAI47_06385: IAI47_06385 - NADH-quinone oxidoreductase subunit NuoG, at 1,298,869 to 1,301,592
_06385
IAI47_06390: IAI47_06390 - NADH-quinone oxidoreductase subunit NuoH, at 1,301,589 to 1,302,566
_06390
IAI47_06395: IAI47_06395 - NADH-quinone oxidoreductase subunit NuoI, at 1,302,578 to 1,303,120
_06395
Position (kb)
1298
1299
1300
1301
1302 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 1297.890 kb on - strand, within IAI47_06380 at 1297.952 kb on + strand, within IAI47_06380 at 1297.953 kb on - strand, within IAI47_06380 at 1297.953 kb on - strand, within IAI47_06380 at 1298.075 kb on - strand, within IAI47_06380 at 1298.138 kb on + strand, within IAI47_06380 at 1298.569 kb on + strand, within IAI47_06380 at 1298.705 kb on + strand at 1298.705 kb on + strand at 1298.705 kb on + strand at 1298.705 kb on + strand at 1298.706 kb on - strand at 1298.757 kb on + strand at 1298.757 kb on + strand at 1298.823 kb on + strand at 1298.823 kb on + strand at 1298.823 kb on + strand at 1298.823 kb on + strand at 1298.823 kb on + strand at 1298.823 kb on + strand at 1298.824 kb on - strand at 1298.824 kb on - strand at 1298.830 kb on + strand at 1298.852 kb on + strand at 1298.869 kb on - strand at 1298.869 kb on - strand at 1298.875 kb on - strand at 1298.885 kb on + strand at 1299.224 kb on + strand, within IAI47_06385 at 1299.225 kb on - strand, within IAI47_06385 at 1299.321 kb on + strand at 1299.350 kb on - strand, within IAI47_06385 at 1299.385 kb on + strand, within IAI47_06385 at 1299.406 kb on + strand, within IAI47_06385 at 1299.406 kb on + strand, within IAI47_06385 at 1299.407 kb on - strand, within IAI47_06385 at 1299.493 kb on - strand, within IAI47_06385 at 1299.573 kb on + strand, within IAI47_06385 at 1299.574 kb on - strand at 1299.638 kb on + strand, within IAI47_06385 at 1299.638 kb on + strand, within IAI47_06385 at 1299.639 kb on - strand, within IAI47_06385 at 1299.639 kb on - strand, within IAI47_06385 at 1299.646 kb on + strand, within IAI47_06385 at 1299.682 kb on + strand, within IAI47_06385 at 1299.741 kb on + strand, within IAI47_06385 at 1299.840 kb on + strand, within IAI47_06385 at 1299.841 kb on - strand, within IAI47_06385 at 1299.889 kb on - strand, within IAI47_06385 at 1299.952 kb on + strand, within IAI47_06385 at 1299.952 kb on + strand, within IAI47_06385 at 1299.952 kb on + strand, within IAI47_06385 at 1299.955 kb on - strand, within IAI47_06385 at 1300.077 kb on + strand at 1300.122 kb on + strand, within IAI47_06385 at 1300.208 kb on - strand, within IAI47_06385 at 1300.781 kb on - strand, within IAI47_06385 at 1300.988 kb on + strand, within IAI47_06385 at 1300.989 kb on - strand, within IAI47_06385 at 1300.993 kb on - strand, within IAI47_06385 at 1300.993 kb on - strand, within IAI47_06385 at 1301.216 kb on + strand, within IAI47_06385 at 1301.559 kb on + strand at 1301.795 kb on + strand, within IAI47_06390 at 1301.819 kb on + strand, within IAI47_06390 at 1301.820 kb on - strand, within IAI47_06390 at 1301.831 kb on + strand, within IAI47_06390 at 1301.885 kb on + strand, within IAI47_06390 at 1301.970 kb on + strand, within IAI47_06390 at 1302.054 kb on + strand, within IAI47_06390 at 1302.188 kb on + strand at 1302.313 kb on - strand, within IAI47_06390 at 1302.313 kb on - strand, within IAI47_06390 at 1302.422 kb on + strand, within IAI47_06390 at 1302.423 kb on - strand, within IAI47_06390 at 1302.423 kb on - strand, within IAI47_06390 at 1302.592 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,297,890 - IAI47_06380 0.35 +4.5 1,297,952 + IAI47_06380 0.40 +0.6 1,297,953 - IAI47_06380 0.40 +4.4 1,297,953 - IAI47_06380 0.40 +2.6 1,298,075 - IAI47_06380 0.49 -1.6 1,298,138 + IAI47_06380 0.54 +2.0 1,298,569 + IAI47_06380 0.86 +2.4 1,298,705 + +0.6 1,298,705 + +2.2 1,298,705 + +2.6 1,298,705 + +2.9 1,298,706 - +5.6 1,298,757 + +0.2 1,298,757 + -0.7 1,298,823 + +0.1 1,298,823 + -0.3 1,298,823 + +0.6 1,298,823 + -1.4 1,298,823 + -0.4 1,298,823 + +0.5 1,298,824 - +0.9 1,298,824 - +3.2 1,298,830 + +1.2 1,298,852 + +1.5 1,298,869 - +2.2 1,298,869 - +4.0 1,298,875 - +4.2 1,298,885 + +2.4 1,299,224 + IAI47_06385 0.13 +3.7 1,299,225 - IAI47_06385 0.13 +4.2 1,299,321 + +0.1 1,299,350 - IAI47_06385 0.18 +2.2 1,299,385 + IAI47_06385 0.19 +0.2 1,299,406 + IAI47_06385 0.20 +3.6 1,299,406 + IAI47_06385 0.20 +4.2 1,299,407 - IAI47_06385 0.20 +3.7 1,299,493 - IAI47_06385 0.23 +3.5 1,299,573 + IAI47_06385 0.26 +2.7 1,299,574 - +4.0 1,299,638 + IAI47_06385 0.28 +3.3 1,299,638 + IAI47_06385 0.28 +4.2 1,299,639 - IAI47_06385 0.28 +0.9 1,299,639 - IAI47_06385 0.28 +4.0 1,299,646 + IAI47_06385 0.29 +1.1 1,299,682 + IAI47_06385 0.30 +3.7 1,299,741 + IAI47_06385 0.32 +2.1 1,299,840 + IAI47_06385 0.36 +2.1 1,299,841 - IAI47_06385 0.36 +3.7 1,299,889 - IAI47_06385 0.37 +4.2 1,299,952 + IAI47_06385 0.40 +1.7 1,299,952 + IAI47_06385 0.40 +3.4 1,299,952 + IAI47_06385 0.40 +4.8 1,299,955 - IAI47_06385 0.40 +3.5 1,300,077 + +1.7 1,300,122 + IAI47_06385 0.46 +2.2 1,300,208 - IAI47_06385 0.49 +4.2 1,300,781 - IAI47_06385 0.70 +6.0 1,300,988 + IAI47_06385 0.78 +4.3 1,300,989 - IAI47_06385 0.78 +3.2 1,300,993 - IAI47_06385 0.78 +1.9 1,300,993 - IAI47_06385 0.78 +2.7 1,301,216 + IAI47_06385 0.86 +0.9 1,301,559 + +3.2 1,301,795 + IAI47_06390 0.21 +4.8 1,301,819 + IAI47_06390 0.24 +3.8 1,301,820 - IAI47_06390 0.24 +2.7 1,301,831 + IAI47_06390 0.25 +4.7 1,301,885 + IAI47_06390 0.30 +2.8 1,301,970 + IAI47_06390 0.39 +2.8 1,302,054 + IAI47_06390 0.48 +3.5 1,302,188 + +1.7 1,302,313 - IAI47_06390 0.74 +2.3 1,302,313 - IAI47_06390 0.74 +3.2 1,302,422 + IAI47_06390 0.85 -0.3 1,302,423 - IAI47_06390 0.85 +4.4 1,302,423 - IAI47_06390 0.85 +4.5 1,302,592 + +1.2
Or see this region's nucleotide sequence